As an eco-friendly adsorption material, hydroxyapatite (Ha) have received widely attention from researchers owing to their excellent biocompatibility and adsorption performance. However, the inconvenient in separating Ha powder from adsorbed processes following use has limited their application. Herein, a novel alginate-based composite beads encapsulation with cellulose and Ha (named HCA) was designed to remove Cu(II) from aqueous solution. Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS) were used for characteristic analysis. The impacts of samples compositions, various Cu(II) concentration, adsorption equilibrium time, and regeneration performance on the adsorption process were investigated. The results suggested that beads exhibited their maximum adsorption capacity for Cu(II) was obtained to be 64.14 mg/g at pH=5 for 8 h, best fitted into Langmuir isotherm models and the pseudo-second-order kinetic model. In addition, the biocompatible beads not only increase the sorption sites, but also have good regenerability, would be a promising bio-adsorbent for heavy metal ion removal.