Novel alleles at the HLA‐DRB1 and ‐DQB1 loci

Lázaro, A. M.; Xiao, Yi; Masaberg, Carly; Lebeck, Lauralynn K.; Ng, Jennifer; Hurley, Carolyn Katovich

doi:10.1111/j.1399-0039.2008.01070.x

Cited by 16 publications

(21 citation statements)

References 5 publications

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“… a Exon 2 of HLA‐DRB1 was characterized by DNA sequencing. HLA‐DRB1 alleles were amplified separately from genomic DNA with group‐specific HLA‐DRB1 primers and identified using DNA sequencing as previously described (6). …”

Section: Description Of Individuals With Novel Hla‐a Allelesmentioning

confidence: 99%

Increased HLA class I and II diversity as 72 novel alleles are identified in volunteers for the National Marrow Donor Program Registry in 2010

Lázaro

Henry

et al. 2011

Tissue Antigens

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Seventy-two novel human leukocyte antigen (HLA) class I and class II alleles are described from volunteers for the 'Be The Match Registry®': 17 HLA-A alleles, 12 HLA-C alleles, 31 HLA-B alleles and 12 HLA-DRB1 alleles. Forty-six (≈ 64%) of the 72 novel alleles are single-nucleotide substitution variants when compared with their most homologous allele. Five of these single-nucleotide variants are silent substitutions and one creates a non-expressed allele (B*44:108N). The remaining novel alleles differ from their most similar allele by two to five nucleotide substitutions. One of the novel HLA-C alleles (C*07:150Q) is of questionable expression due to an insertion of 21 nucleotides starting at codon 143 that adds seven amino acids to exon 3. An inter-locus gene conversion may have created the novel allele HLA-A*23:31 that shares its codon differences with HLA-B*07:28. Some of the new alleles encode novel codons and unique amino acid changes at polymorphic positions in the HLA-A (codons 116 and 150), HLA-C (codon 114), HLA-B (codons 11, 21, 35, 42, 48, 73, 98 and 170) and HLA-DRB1 (codon 29) loci.

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Section: Description Of Individuals With Novel Hla‐a Allelesmentioning

confidence: 99%

Increased HLA class I and II diversity as 72 novel alleles are identified in volunteers for the National Marrow Donor Program Registry in 2010

Lázaro

Henry

et al. 2011

Tissue Antigens

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“…Human leukocyte antigen (HLA)‐DRB1 alleles were isolated by cloning of a locus‐specific amplicon (1). New DRB1 alleles were isolated with DRB1*03/08/11/12/13/14‐specific primers located in DRB1 introns 1 and 2, respectively (2, 3). DNA sequencing of both strands was performed using M13 primers, Prism Big Dye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA), a 3730 xl sequencer (Applied Biosystems).…”

Section: Cells and Description Of Novel Sequencesmentioning

confidence: 99%

Identification of new HLA‐DRB1 alleles in Kazakh individuals

2011

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Section: Description Of Individuals With Novel Human Leukocyte Antigementioning

confidence: 99%

“…HLA‐DPB1 alleles were amplified from genomic DNA with generic DPB1 primers (9) and cloned. The HLA‐DPB1 alleles were identified from clones by SBT (8) using primers described in reference (9).…”

Section: Description Of Individuals With Novel Human Leukocyte Antigementioning

confidence: 99%

Seventy‐eight novel HLA class I and II alleles identified during routine registry typing in 2008 and 2009

et al. 2010

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Seventy-eight novel human leukocyte antigen (HLA) class I and class II alleles are described: 19 HLA-A alleles, 30 HLA-C alleles, 21 HLA-B alleles, 7 HLA-DRB1 alleles and 1 HLA-DPB1 allele. Eight (∼10%) of the novel alleles were found in more than one individual and may be more common in the population. Sixty-two (∼80%) of the 78 novel alleles are single nucleotide substitution variants when compared with their most homologous allele. Twelve of these single nucleotide variants are silent substitutions and one creates a null allele (C*08:26N). One of the novel HLA-C alleles, C*03:58, is a hybrid that likely resulted from an intra-locus recombination. The remaining novel alleles differ from their most similar allele by two to seven nucleotide substitutions. Four of the novel HLA-C alleles encode amino acid changes at codons 41, 42, 55 or 200 which have not previously been reported to be polymorphic. Some of the new alleles encode novel codons and unique amino acid changes at polymorphic positions in the HLA-A (codons 55 and 120), HLA-C (codons 151, 153 and 176), HLA-B (codons 31 and 84) and HLA-DRB1 (codon 47) loci.

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Novel alleles at the HLA‐DRB1 and ‐DQB1 loci

Cited by 16 publications

References 5 publications

Increased HLA class I and II diversity as 72 novel alleles are identified in volunteers for the National Marrow Donor Program Registry in 2010

Increased HLA class I and II diversity as 72 novel alleles are identified in volunteers for the National Marrow Donor Program Registry in 2010

Identification of new HLA‐DRB1 alleles in Kazakh individuals

Seventy‐eight novel HLA class I and II alleles identified during routine registry typing in 2008 and 2009

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