2021
DOI: 10.3390/microorganisms9102078
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Novel and Conventional Isolation Techniques to Obtain Planctomycetes from Marine Environments

Abstract: Bacteria from the distinctive Planctomycetes phylum are well spread around the globe; they are capable of colonizing many habitats, including marine, freshwater, terrestrial, and even extreme habitats such as hydrothermal vents and hot springs. They can also be found living in association with other organisms, such as macroalgae, plants, and invertebrates. While ubiquitous, only a small fraction of the known diversity includes axenic cultures. In this study, we aimed to apply conventional techniques to isolate… Show more

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Cited by 15 publications
(22 citation statements)
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References 71 publications
(133 reference statements)
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“…S1). After plate incubation for 45 days at room temperature and in darkness, the content of each pit was re-inoculated into modified M13 medium plates [8,46] and incubated at 25 °C. A colony with beige pigmentation assigned as strain ICM_H10 T was obtained after 3 weeks incubation.…”
Section: Isolation and Ecologymentioning
confidence: 99%
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“…S1). After plate incubation for 45 days at room temperature and in darkness, the content of each pit was re-inoculated into modified M13 medium plates [8,46] and incubated at 25 °C. A colony with beige pigmentation assigned as strain ICM_H10 T was obtained after 3 weeks incubation.…”
Section: Isolation and Ecologymentioning
confidence: 99%
“…Water submerged sediments were collected in Memória beach (Matosinhos), Portugal (41° 13′ 50.35″ N 8° 43′ 16.61″ W) [46] (Fig. S1, available in the online version of this article) in October 2020 during a low tide and brought immediately into the laboratory in a cold chamber.…”
Section: Isolation and Ecologymentioning
confidence: 99%
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