Novel Antimicrobial Coating on Silicone Contact Lens Using Glycidyl Methacrylate and Polyethyleneimine Based Polymers

Abstract

Contact lenses are medical devices commonly used to correct refractive errors and to maintain ocular health. Microorganisms such as bacteria that grow on the lens surface cause irritation to the eyes and can even cause loss of vision. In this paper, two different coating strategies are employed to form an efficient antimicrobial coating on contact lenses. In the first method, a presynthesized copolymer of polyethyleneimine‐graft‐polyethylene glycol methacrylate (PEI‐PEGMA) is used and the coated lenses show antimicrobial activity (in vitro) against methicillin‐resistant Staphylococcus aureus (MRSA) bacteria with killing efficacy >99.99% and log reduction of 5.1 and proxy host cell viability of 79%. In the second method, commercially available monomers/polymers such as glycidyl methacrylate (GMA), sulfobetaine methacrylate, and polyethyleneimine are used. A typical formulation consisting of 1% GMA shows antibacterial activity against MRSA with killing efficacy >99.99% and log reduction of 6.3. Proxy host cell viability for the coated lenses is found to be 90% indicating that the coating is nontoxic. Antibacterial coating reported here is very effective in killing gram‐positive bacteria such as MRSA and S. aureus. The second method using commercially available monomers/polymers involving a simple coating procedure is also easily scalable.

A Novel Strategy for Creating an Antibacterial Surface Using a Highly Efficient Electrospray-Based Method for Silica Deposition

“…High biocompatibility of our fabricated surface was observed by more than 90% HDF cells under 24 h exposure of extracted coating materials ( Figure 6 A). These findings were consistent with the results of a previous study by Pillai et al [ 59 ] who performed the same extraction method and showed that cell viability remained at greater than 80%; thus, these findings suggested that the coating materials did not diffuse out into the growth medium and exhibited excellent biocompatibility without toxicity in HDFs. In addition, our findings were similar to those of Dulski et al who focused on the development of silver-silica coating as a functional biomaterial surface [ 60 ].…”

“…Cytotoxicity tests were performed using MTT assays (Invitrogen Corporation, Carlsbad, CA, USA) in 96-well plates, as described previously [ 59 ]. Conditioned medium was prepared by incubating cells in growth medium for 24 h. The growth medium in 96-well plates was aspirated, and cells were washed with 1X PBS and incubated with conditioned medium for an additional 24 h. MTT assays were then performed by mixing 10 µL of 5 mg/mL MTT in 100 µL growth medium in each well, followed by a 2 h incubation.…”

Synthesis and Characterization of Cationic Dendrimer‐PDMS Hybrids

“…[2,[5][6][7] One approach to fabricate silicones resistant to biofilm is to make their surfaces antimicrobial. While several methods to render silicone surfaces antimicrobial exist, including through coatings, [8][9][10][11][12][13][14][15] additives, [16][17][18][19] or surface functionalization, [20][21][22][23][24] each has advantages and disadvantages. Coatings and surface functionalization place antimicrobials directly at environmental interfaces but require multistep processing during device manufacturing.…”

Benchtop Preparation of Polymer Brushes by SI-PET-RAFT: The Effect of the Polymer Composition and Structure on Inhibition of a Pseudomonas Biofilm

“…As expected, PDEGA brushes showed decreasing biofilm attachment as the brush thickness increased (Figure S10D). For the methacrylates, 8 out of the 10 monomers were not able to reduce bacterial cell attachments under 50%, including the zwitterionic PDMAPS and PMPC brushes, which have been well regarded for their antifouling performances , (Figure C). The less than ideal performance of these brushes may be attributed to the low grafting density of our system (0.2 chain/nm 2 ). , Following the trend observed for the acrylates, POEGMA- and PTFEMA-brushed surfaces afforded the lowest biofilm attachments of 37 and 31%, respectively.…”