Novel Antioxidant Peptides Derived from Enzymatic Hydrolysates of Macadamia Protein

Han, Zhiping; Zhang, Wenhua; Luo, Wenqiong; Li, Jihua

doi:10.4236/jbm.2016.42002

Cited by 3 publications

(2 citation statements)

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“…The TAC of WGPH samples increased with concentration (300–2,000 µg/ml) (Figure ). This finding is in agreement with previous studies reporting better TAC at higher WGPH concentration (Chen et al, ; Han, Zhang, Luo, & Li, ). TAC displayed a BHT > WGPH order at all concentrations, consistent with results by Bougatef et al ().…”

Section: Resultssupporting

confidence: 94%

Identification and synthesis of multifunctional peptides from wheat germ hydrolysate fractions obtained by proteinase K digestion

et al. 2019

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Wheat germ protein hydrolysate (WGPH) was obtained by proteinase K digestion, in order to produce bioactive antioxidant and antihypertensive peptides. Response surface methodology (RSM) was used to optimize hydrolysis conditions (enzyme‐to‐substrate ratio, time, and temperature) for antioxidant activity of hydrolysates. The crude WGPH produced in this way significantly inhibited angiotensin‐I converting enzyme (ACE) in a concentration‐dependent manner. It was next fractionated by reversed‐phase semi‐preparative High Performance Liquid Chromatography (HPLC) into 12 fractions that were examined for antioxidant and antihypertensive activities. Fractions with antioxidant and ACE‐inhibitory activities were then submitted to further analysis by nano‐LC‐ESI‐MS‐MS. Among the various peptides identified, MDATALHYENQK (IC50: 293.3 ± 6.5 µg/ml) and SGGSYADELVSTAK (IC50: 265.5 ± 8.3 µg/ml) displayed antioxidant activity and VALTGDNGHSDHVVHF (IC50: 189.3 ± 4.05 µg/ml), VDSLLTAAK (IC50: 159.7 ± 0.33 µg/ml), MDATALHYENQK (IC50: 303.6 ± 2.47 µg/ml), IGGIGTVPVGR (IC50: 125.7 ± 2.3 µg/ml) and SGGSYADELVSTAK (IC50: 128.2 ± 1.17 µg/ml) showed good ACE‐inhibitory activity. Practical applications Wheat milling industries produce massive amounts of wheat germ as by‐product that can be converted into valuable compounds. The present research indicates that proteinase K is useful to hydrolyze wheat germ proteins in a search for bioactive peptides with antioxidant and ACE‐inhibitory properties. The identified peptides can be regarded as functional food additives, or nutraceuticals to improve human health.

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Section: Resultssupporting

confidence: 94%

Identification and synthesis of multifunctional peptides from wheat germ hydrolysate fractions obtained by proteinase K digestion

et al. 2019

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“…Damez dan Clerjon (2008) menjelaskan bahwa struktur jaringan ikat hewan yang terdiri dari tropokolagen dan polipeptida dengan struktur triple-α-heliks secara fisik membentuk jaringan yang keras dan padat serta sulit larut dalam air. Enzim papain merupakan jenis eksoenzim yang mempunyai gugus sulfhidril mampu berfungsi sebagai proteolitik struktur triple-α-heliks menjadi peptida sederhana secara irreversible yang menjadikan protein dapat larut dalam air (Han et al, 2016). Peptida yang lebih sederhana dapat berpotensi mengerahkan fungsi antioksidan secara baik (Chandrasekaran et al, 2016).…”

Section: Tabel 1 Komposisi Kimia Bubuk Ceker Ayamunclassified

Optimasi Aktivitas Antioksidan Peptida Aktif dari Ceker Ayam Melalui Hidrolisis Enzim Papain

Susanto

Rosyidi

Radiati

et al. 2018

JITEK

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The objective of this study was to optimization antioxidant activity of active peptides of chicken feet with papain hydrolysis. The research method was experimental design conducted using Factorial Completely Randomized Design. Treatments included papain concentration (control (0%) , 1%, 2% and 4%) and Incubation time (24 hours, 36 hours and 48 hours) were repeated four times. The variables observed were proximate analysis,microstructure, dissolved protein concentration and antioxidant activity. The results indicate that the treatment of papain concentration and incubation time variations in chicken feet protein extraction gave a differenceinfluence (P <0.05) to the concentration of solubility protein and antioxidant activity. The interaction of 3% papain concentration and 36 hours incubation time resulted in the highest antioxidant activity amount 55.10 ± 2.24 %. It could be concluded that chicken feet produced bioctive peptide compound that gave high antioxidant activity optimized by hydrolysis of papain enzymes thereby increasing their functional value.

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The Impact of Brevinin-2R Peptide on Oxidative Statues and Antioxidant Enzymes in Human Epithelial Cell Line of A549

Ghodsi-Moghadam

Asoodeh

2018

Int J Pept Res Ther

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Novel Antioxidant Peptides Derived from Enzymatic Hydrolysates of Macadamia Protein

Cited by 3 publications

References 30 publications

Identification and synthesis of multifunctional peptides from wheat germ hydrolysate fractions obtained by proteinase K digestion

Identification and synthesis of multifunctional peptides from wheat germ hydrolysate fractions obtained by proteinase K digestion

Optimasi Aktivitas Antioksidan Peptida Aktif dari Ceker Ayam Melalui Hidrolisis Enzim Papain

The Impact of Brevinin-2R Peptide on Oxidative Statues and Antioxidant Enzymes in Human Epithelial Cell Line of A549

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