Novel approaches of erythrosine B as a food dye‐derived spectroscopic probe for assessing trospium chloride in raw material and dosage form

Abstract

Two facile spectroscopic methodologies were designed for estimating trospium chloride (TPM) in raw material and tablets with high operational reliability and selectivity. The methods were based on using erythrosine B (EB) as a spectroscopic tool for ion-pair complex formation with the drug. In a mild acidic medium of Britton Robinson buffer (pH 4.0), the ionized hydroxyl group in the reagent interacted with the ionized amine in the studied drug. Method I was based on the spectrophotometric measuring of the absorbance of the reaction product at 557 nm. Method II was based on spectrofluorimetric measurement of the quenching effect of TPM on the inherent fluorescence of EB at 550 nm (λ ex. = 528 nm).The two methods showed linearity through ranges 1.

Green spectrophotometric and spectrofluorimetric determination of biperiden hydrochloride using erythrosine B sensing probe

“…It was found that the maximum absorbance was produced at pH 4 (Figure 4). Most studies of EB quenching by basic drugs proceed in the acidic medium [15][16][17][18]. Different volumes of BR buffer (0.3-2.0 mL) at pH 4 were examined.…”

“…Based on its chemical structure, it is an acidic dye that can form ion-pair complexes with basic amino compounds such as BIP. For instance, it has been employed for spectrofluorimetric and spectrophotometric determination of albendazole [14], benzimidazole [15], varenicline [16], trospium chloride [17], tamoxifen [18], sunitinib [19], rupatadine [20], and naftidrofuryl [21].…”

Validated spectrofluorimetric and resonance Rayleigh scattering methods for determining naftidrofuryl in varied pharmaceutical samples based on its interaction with erythrosin B

“…Recently, xanthene dyes (eosin Y, erythrosin B, fluorescein, merbromin) have been used as colorimetric, fluorescent, and/or RRS analytical reagents for determining a wide range of active pharmaceutical ingredients [12]. For example, eosin Y and merbromin dyes have been reported for determining daclatasvir, cyclobenzaprine, fluvoxamine, atomoxetine, oxybutynin, dexlansoprazole and some β-blockers [13][14][15][16][17][18][19], while erythrosin B has been utilized for determining rupatadine, duloxetine, terbinafine, venlafaxine, nilotinib trospium, and varenicline [20][21][22][23][24][25][26]. The benefits of using erythrosin B include its low price and a high degree of stability.…”