Novel approaches to characterize age‐related remodelling of the dermal‐epidermal junction in 2D, 3D and <i>in vivo</i>

Newton, Victoria Louise; Bradley, Robert S.; Séroul, Pierre; Cherel, Marie; Griffiths, C.E.M.; Rawlings, A. V.; Voegeli, R.; Watson, Rachel; Sherratt, Michael J.

doi:10.1111/srt.12312

Cited by 32 publications

(33 citation statements)

References 54 publications

(72 reference statements)

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“…To see the age‐dependent changes in the skin, we compared histological characteristics of abdominal skin from young (24 years) and aged (65 years) individuals. Consistent with the previous observations, aged skin exhibited flattened and thin epidermis along with structurally altered dermal papillae/rete ridges and collagen bundles in the dermis (Figure A).…”

Section: Resultssupporting

confidence: 92%

Unripe peach (Prunus persica) extract ameliorates damage from UV irradiation and improved collagen XVIII expression in 3D skin model

Yonezawa

Momota

Iwano³

et al. 2018

J of Cosmetic Dermatology

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Summary Introduction Collagen type XVIII regulates cellular activities of adjacent cells at the dermal‐epidermal junction (DEJ). To investigate its possible changes during aging, we compared its mRNA levels and protein localization in skin samples from female participants aged 20‐70 years old. In addition, we evaluated the beneficial effects of unripe peach extracts in a 3D skin model. Methods Sun‐exposed or sun‐protected female skin samples were compared by DNA array or by immunohistochemistry for basement membrane components. To evaluate protective effects of fresh unripe peach extract, UV‐B irradiated human 3D skin models were incubated in the presence or absence of the extract, followed by measurements of mRNA levels by real‐time PCR, or by immunohistochemistry. Results In aged skin samples, COL18A1 mRNA levels were lower and the protein localization exhibited less intensive signal by anti‐collagen type XVIII immunostaining. As observed in the skin tissues, collagen type XVIII exists at the DEJ in the 3D skin model. Fresh unripe peach extract significantly improved mRNA levels and partially localizations of collagen type XVIII, suggesting that fresh unripe peach extract ameliorates DEJ damages caused by UV‐B irradiation. Conclusion Collagen type XVIII and fresh unripe peach extract can be promising protective cosmetic strategies against skin aging.

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Section: Resultssupporting

confidence: 92%

Unripe peach (Prunus persica) extract ameliorates damage from UV irradiation and improved collagen XVIII expression in 3D skin model

Yonezawa

Momota

Iwano³

et al. 2018

J of Cosmetic Dermatology

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“…4e) could be attributed to melanin-containing cells 52 . We note that visualization of the rete ridges by separating fast melanin fluorescence in FLIM (blue color in the Video 3) could be used to reconstruct the morphology of the dermal-epidermal junction, which is indicative of skin pathologies 64 .…”

Section: Resultsmentioning

confidence: 99%

Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization

Shirshin

Gurfinkel

Priezzhev

et al. 2017

Sci Rep

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The papillary dermis of human skin is responsible for its biomechanical properties and for supply of epidermis with chemicals. Dermis is mainly composed of structural protein molecules, including collagen and elastin, and contains blood capillaries. Connective tissue diseases, as well as cardiovascular complications have manifestations on the molecular level in the papillary dermis (e.g. alteration of collagen I and III content) and in the capillary structure. In this paper we assessed the molecular structure of internal and external regions of skin capillaries using two-photon fluorescence lifetime imaging (FLIM) of endogenous compounds. It was shown that the capillaries are characterized by a fast fluorescence decay, which is originated from red blood cells and blood plasma. Using the second harmonic generation signal, FLIM segmentation was performed, which provided for spatial localization and fluorescence decay parameters distribution of collagen I and elastin in the dermal papillae. It was demonstrated that the lifetime distribution was different for the inner area of dermal papillae around the capillary loop that was suggested to be due to collagen III. Hence, we propose a generalized approach to two-photon imaging of the papillary dermis components, which extends the capabilities of this technique in skin diagnosis.

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“…This technique provides rapid and accurate information to measure the volume, the height, the orientation of dermal papillae. The existence of this kind of structure and their evolution with age are always the subject of studies …”

Section: Resultsmentioning

confidence: 99%

3D imaging of human epidermis micromorphology by combining fluorescent dye, optical clearing and confocal microscopy

Fernandez

Marull-Tufeu

2019

Skin Research and Technology

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Background The human epidermis presents a complex organization due to its dynamic and plein‐stratified epithelium. Still nowadays, one of the best method to study this layer of the skin remains the histology sections. This approach remains tedious and gives only 2D information. Materials and Methods Rhodamine B is a dye known to have a high affinity for the epidermal layer and to possess fluorescent properties. Associated with a clarification method such as 3DiSCO, this dye maintains a high fluorescence emission. The skin which became transparent can then be investigated with a Laser Scanning Confocal Microscope. Results We showed that this technique can collect longitudinal or transversal optical sections of the epidermis as whole. Serial sections allowed to move easily into the epidermis. A 3D imaging can also be generated to study the microrelief of the stratum corneum or the complex organization of the dermal‐epidermal junction. Conclusion In this work, we describe a simple and fast staining and clearing method for skin samples. In association with a fluorescence microscope such as the confocal, we show a new way to characterize the whole epidermis. This work appears as a valuable and complementary method to understand several topics around skin investigation.

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Novel approaches to characterize age‐related remodelling of the dermal‐epidermal junction in 2D, 3D and in vivo

Cited by 32 publications

References 54 publications

Unripe peach (Prunus persica) extract ameliorates damage from UV irradiation and improved collagen XVIII expression in 3D skin model

Unripe peach (Prunus persica) extract ameliorates damage from UV irradiation and improved collagen XVIII expression in 3D skin model

Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization

3D imaging of human epidermis micromorphology by combining fluorescent dye, optical clearing and confocal microscopy

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