Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression

Sakurai, Hiroshi; Enoki, Yasuaki

doi:10.1111/j.1742-4658.2010.07829.x

Cited by 109 publications

(102 citation statements)

References 80 publications

(144 reference statements)

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“…DBD is the most conserved component of Hsfs, consisting of an antiparallel 4-stranded b-sheet (b1, b2, b3, b4) packed against a bundle of 3 a-helices (H1, H2, H3) (Damberger et al, 1994). The hydrophobic core of this domain ensures the specific recognition of heat stress promoter elements (Littlefield and Nelson, 1999;Sakurai and Enoki, 2010). Based on differences in their oligomerization domains, plant Hsf protein families fall into 3 classes ( A, B, and C).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification, classification, and analysis of heat shock transcription factor family in Chinese cabbage (Brassica rapa pekinensis)

Huang¹,

Peng²,

Li³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Chinese cabbage (Brassica rapa ssp. pekinensis) is one of the most important vegetable crops grown worldwide, and various methods exist for selection, propagation, and cultivation. The entire Chinese cabbage genome has been sequenced, and the heat shock transcription factor family (Hsfs) has been found to play a central role in plant growth and development and in the response to biotic and abiotic stress conditions, particularly in acquired thermotolerance. We analyzed heat tolerance mechanisms in Chinese cabbage. In this study, 30 Hsfs were identified from the Chinese cabbage genome database. The classification, phylogenetic reconstruction, chromosome distribution, conserved motifs, expression analysis, and interaction networks of the Hsfs were predicted and analyzed. Thirty BrHsfs were classified into 3 major classes (class A, B, and C) according to their structural characteristics and phylogenetic comparisons, and class A was further subdivided into 8 subclasses. Distribution mapping results showed that Hsf genes were located on 10 Chinese cabbage chromosomes. The expression profile indicated that Hsfs play differential roles in 5 organs in Chinese cabbage, and likely participate in the development of underground parts and regulation of reproductive growth. An orthologous gene interaction network was constructed, and included MBF1C, ROF1, TBP2, CDC2, and HSP70 5 genes, which are closely related to heat stress. Our results contribute to the understanding of the complexity of Hsfs in Chinese cabbage and provide a basis for further functional gene research.

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Section: Introductionmentioning

confidence: 99%

Genome-wide identification, classification, and analysis of heat shock transcription factor family in Chinese cabbage (Brassica rapa pekinensis)

Huang¹,

Peng²,

Li³

et al. 2015

Genet. Mol. Res.

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“…Heat shock proteins generally function as molecular chaperones, and Hsp82 can stabilize genetic variants of proteins, permitting the evolution of new phenotypes ranging from cancer to antifungal drug resistance (6,39). Hsp82 binds to heat shock factor (Hsf), a client protein that orchestrates the transcription of stress response proteins and maintains Hsf in an inactive state (31). Other Hsp82 clients are linked to signal transduction, transcription factors, and chromatin remodeling, which can dramatically influence cellular phenotypes (37).…”

Section: Discussionmentioning

confidence: 99%

Discovery of a Role for Hsp82 in Histoplasma Virulence through a Quantitative Screen for Macrophage Lethality

2011

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The application of forward genetics can reveal new factors required for the virulence of intracellular pathogens. To facilitate such virulence screens, we developed macrophage cell lines with which the number of intact host cells following infection with intracellular pathogens can be rapidly and easily ascertained through the expression of a constitutive lacZ transgene. Using known virulence mutants of Francisella novicida and Histoplasma capsulatum, we confirmed the applicability of these host cells for the quantitative assessment of bacterial and fungal virulence, respectively. To identify new genes required for Histoplasma virulence, we employed these transgenic macrophage cells to screen a collection of individual transfer DNA (T-DNA) insertion mutants. Among the mutants showing decreased virulence in macrophages, we identified an insertion in the locus encoding the Histoplasma Hsp82 homolog. The lesion caused by the T-DNA insertion localizes to the promoter region, resulting in significantly decreased HSP82 expression. Reduced HSP82 expression markedly attenuates the virulence of Histoplasma yeast in vivo. While the HSP82 hypomorph grows normally in vitro at 37°C and under acid and salinity stresses, its ability to recover from high-temperature stress is impaired. These results provide genetic proof of the role of stress chaperones in the virulence of a thermally dimorphic fungal pathogen.

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“…Moreover, the expression of HSPs is controlled by the HSFs, with a conserved DNA binding domain (central helix-turn-helix motif) that enables binding to the heat shock elements (HSEs) in the target promoter (Scharf et al, 1998, Sakurai andEnoki, 2010). Rice has 19 HSFs that have been cloned and characterized (Morimoto, 1998).…”

Section: Gene Discovery and Regulatory Mechanismsmentioning

confidence: 99%

When water runs dry and temperature heats up: Understanding the mechanisms in rice tolerance to drought and high temperature stress conditions

Rabara¹,

Msanne²,

Ferrer³

et al. 2018

Preprint

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Rice production, owing to its high-water requirement for cultivation, is very vulnerable to the threat of changing climate, particularly prolonged drought and high temperature. Such threats heighten the need for abiotic stress-resilient rice varieties with better yield potential. This review examines the physiological and molecular mechanisms of rice varieties to cope with stress conditions of drought (DS), high temperature (HTS) and their combination (DS-HTS). It appraises research studies in rice about its various phenotypic traits, genetic loci and response mechanisms to stress conditions to help craft new breeding strategies for rice varieties with improved resilience to abiotic stresses. This review consolidates available information on promising rice cultivars with desirable traits as well as advocates synergistic and complementary approaches in molecular and systems biology to develop new rice breeds that favorably respond to climate-induced abiotic stresses. The development of new breeding and cultivation strategies for climate-resilient rice varieties is a challenging task. It requires a comprehensive understanding of the various morphological, biochemical, physiological, and molecular components governing yield under drought and high temperature, but possible by implementing cohesive approaches involving molecular and systems biology approaches in genomics and molecular breeding, including genetic engineering.

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Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression

Cited by 109 publications

References 80 publications

Genome-wide identification, classification, and analysis of heat shock transcription factor family in Chinese cabbage (Brassica rapa pekinensis)

Genome-wide identification, classification, and analysis of heat shock transcription factor family in Chinese cabbage (Brassica rapa pekinensis)

Discovery of a Role for Hsp82 in Histoplasma Virulence through a Quantitative Screen for Macrophage Lethality

<strong>When water runs dry and temperature heats up: </strong><strong>Understanding the mechanisms in rice tolerance to drought and </strong><strong>high temperature stress conditions</strong>

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