Novel association between sperm deformity index and oxidative stress-induced DNA damage in infertile male patients

Said, Tamer M.; Aziz, Nabil; Sharma, Rakesh K.; Lewis-Jones, Iwan; Thomas, Anthony J.; Agarwal, Ashok

doi:10.1111/j.1745-7262.2005.00022.x

Cited by 65 publications

(37 citation statements)

References 19 publications

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“…Infertile men with male factor or idiopathic diagnoses had significantly lower ROS-TAC scores than controls (Jedlinska et al, 2006). Besides, Said et al (2005) suggested that abnormal sperm morphology combined with elevated ROS production may serve as a useful indicator of potential damage to sperm DNA. On the other hand, spermatozoa are highly susceptible to damage by excessive concentrations of ROS due to the high content of polyunsaturated fatty acids within their plasma membrane.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, spermatozoa are highly susceptible to damage by excessive concentrations of ROS due to the high content of polyunsaturated fatty acids within their plasma membrane. The lipid peroxidation destroys the structure of lipid matrix in the membranes of spermatozoa, and it is associated with loss of motility and impairment of spermatogenesis (Said et al, 2005;Jedlinska et al, 2006). In the present study, administration of 400 and 600 mg/rat citrus extract for thirty consecutive days significantly increased sperm motility and viability in both experimental groups as compared with the control group, beside this research showed levels of TAC and SOD in both extracts groups were significantly increased and levels of MDA was decreased (p<0.05) and this results were consequential in 400 mg/kg extract (Table 1).…”

Section: Discussionmentioning

confidence: 99%

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Anti-oxidative effects of citro flavonoids on spermatogenesis in rat

Khaki¹

2011

Afr. J. Pharm. Pharmacol.

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Citrus fruits have long been recognized as containing valuable sources of important nutrients which are biologically active in humans. Citrus fruits, such as oranges, contain compounds called phytochemicals that can be included into three major groups: the flavonoids, limonoids and carotenoids. The flavonoids are a group of benzopyran derivatives which occur widely in plants. The flavonoids typically consist of a benzene ring fused with the heterocyclic six-membered ring containing an oxygen atom. As citrus has an antioxidant's potential, we want to evaluating its useful effect on spermatogenesis and sperm parameters. Wistar male rat (n=30) were allocated into three groups, control (n=10) and test groups (n=20), that subdivided into groups of 2 that received citrus extract powder (400 and 600 mg/rat) for 30 consequence day. Animals were kept in standard conditions. In twentieth day the testes tissue of rats in whole groups were removed and sperm was collected from epididymis and prepared for analysis. TAC, SOD levels and percentage of sperm viability and motility in both test groups significantly increased (p<0.05) in comparison to control group, whereas, sperm concentration, morphology and testes weights in both experimental and control group were similar. The level of MDA in both extract groups were significantly decreased (p<0.05). Results revealed that administration of 600 mg/kg/day citrus extract significantly increased the TAC, SOD levels and sperm percentage, viability, motility and decreased MDA levels. This suggested that citrus may be promising in enhancing sperm healthy parameters.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Anti-oxidative effects of citro flavonoids on spermatogenesis in rat

Khaki¹

2011

Afr. J. Pharm. Pharmacol.

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“…Structurally, this is in agreement with our study results of significant interdependence between the percentages of sperm with a cytoplasmic droplet and midpiece defects on one hand and caspase activation and MMP integrity on the other. Our group has previously shown that sperm cytoplasmic droplet deformity is associated with excessive reactive oxygen species (ROS) production (Aziz et al, 2004;Said et al, 2005a). In view of their positive correlation with apoptosis and excessive ROS production, these immature spermatozoa with excessive cytoplasmic remnants could be responsible for male subfertility when present in abundance.…”

Section: Discussionmentioning

confidence: 99%

The relationship between human sperm apoptosis, morphology and the sperm deformity index

et al. 2007

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BACKGROUND: This study aimed to assess the relationship between apoptosis in human ejaculated spermatozoa, sperm morphology and the novel sperm deformity index (SDI). METHODS: Semen specimens from 50 healthy donors were prepared by density-gradient centrifugation followed by incubating the prepared sperm with paramagnetic annexin V-conjugated microbeads and subjecting this to magnetic cell sorting (MACS). The procedure delivers two sperm fractions: annexin-negative (non-apoptotic) and annexin-positive (apoptotic). Activated caspase-3 levels and the integrity of the sperm mitochondrial membrane potential (MMP) were assessed as markers of apoptosis in the annexin-negative and -positive aliquots following MACS. Sperm morphology and the SDI scores were assessed using the strict criteria. RESULTS: Compared with the apoptotic sperm subpopulations, the non-apoptotic sperm subpopulations had an improved sperm morphology profile as demonstrated by significantly higher proportions of sperm with normal morphology and significantly lower SDI scores and percentages of sperm with acrosomal defects, midpiece defects, cytoplasmic droplet and tail defects. There was a significant correlation between sperm morphology attributes studied and the expressed apoptotic markers-caspase-3 activation and MMP integrity. CONCLUSIONS: Non-apoptotic sperm fractions have morphologically superior quality sperm compared with apoptotic fractions as reflected by significantly lower SDI scores. The study results may support abortive apoptosis, where the apoptotic mechanism of sperm is already triggered prior to ejaculation.

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“…141 From studies of different groups, it was found that ROS hamper the integrity of plasma membrane and nuclear DNA, leading to breakage of DNA and chromatin crosslinking. 142 When the level of these breaks is high, it might lead to lowered fertility. 143 Immature, morphologically abnormal spermatozoa and seminal leukocytes are the main sources of ROS in ejaculates.…”

Section: Detection Of Oxidative Stress and Lipid Peroxidationmentioning

confidence: 99%

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Hossain¹,

Johannisson²,

Wallgren³

et al. 2011

Asian J Androl

143

120

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Flow cytometry is now a recognized methodology within animal spermatology, and has moved from being a research tool to become routine in the assessment of animal semen destined to breeding. The availability of 'bench-top' flow cytometers and of newer and versatile markers for cell structure and function had allowed the instrumentation to measure more sperm parameters, from viability to reactiveness when exposed to exogenous stimuli, and to increase our capabilities to sort spermatozoa for potential fertilizing capacity, or chromosomal sex. The present review summarizes the state of the art regarding flow cytometry applied to animal andrology, albeit keeping an open comparative intent. It critically evaluates the present and future capabilities of flow cytometry for the diagnostics of potential fertility and for the development of current reproductive technologies such as sperm freezing, sperm selection and sperm sorting. The flow cytometry methods will probably further revolutionize our understanding of the sperm physiology and their functionality, and will undoubtedly extend its application in isolating many uncharacterized features of spermatozoa. However, continuous follow-up of the methods is a necessity owing to technical developments and the complexity of mapping spermatozoa.

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Novel association between sperm deformity index and oxidative stress-induced DNA damage in infertile male patients

Cited by 65 publications

References 19 publications

Anti-oxidative effects of citro flavonoids on spermatogenesis in rat

Anti-oxidative effects of citro flavonoids on spermatogenesis in rat

The relationship between human sperm apoptosis, morphology and the sperm deformity index

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

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