Novel Au-tetrahedral aptamer nanostructure for the electrochemiluminescence detection of acetamiprid

Point-of-Care Diagnoses and Assays Based on Lateral Flow Test

“…Concurrently, the recognition capability of antibodies suitable for antigens measuring respective antigens when antibodies are assayed is frequently replaced by the use of aptamers by many assays. Extensive progress in aptamer preparation has been reached in recent years, and the aptamers typically comprise ribonucleic acid (RNA), deoxyribonucleic acid (DNA), peptides, or proteins [42][43][44][45][46][47][48].…”

A novel label-free electrochemiluminescence aptasensor using a tetrahedral DNA nanostructure as a scaffold for ultrasensitive detection of organophosphorus pesticides in a luminol–H2O2 system

“…Aptamers are single-stranded oligonucleotides (DNA or RNA) or peptides, which have abilities that are similar to those of the interactions between antigens and antibodies to bind to the target and exhibit high affinity and specificity. 11–14 Nevertheless, aptamers have certain limitations in the application of biosensors. For instance, the orientation and density of single-stranded DNA (ssDNA) aptamers that are fixed on the electrodes are difficult to control, leading to entanglement and aggregation, which extremely inhibit the binding activity of the aptamers and target.…”

“…13,15 Tetrahedral DNA nanostructures (TDNs) with the characteristic of mechanical stiffness and structural stability are easy to functionalize with different inorganic nanoparticles. 12 Thus, TDNs are introduced as ideal scaffolds for controlling aptamers to construct aptasensors so as to overcome these limitations. 16 TDNs offer several virtues that include a well-controlled density, the orientation of DNA bio-probes, minimized nonspecific absorption, and high binding affinity superior to ssDNA.…”

“…25 Guo's group had fixed the Au-tetrahedral aptamer nanostructure on the working electrodes to enhance the function of the aptamers and improved the sensing performance. 12 Related studies, in recent years, have proved that AgNPs can stimulate the transfer of electrons from the reaction center to the electrode surface owing to the stronger electron transfer activity. 19 Wang's group had synthesized a micro flower-like structure made of ultrathin Co/Ni-based metal–organic framework (MOF) nanosheets, whose structure was used as a platform for luminol-functionalized AgNPs to construct an immunosensor for the tumor marker alpha-fetoprotein.…”

Multifunctional polyethyleneimine for synthesis of core-shell nanostructures and electrochemiluminescent detection of three AMI biomarkers

“…Electrochemiluminescence (ECL) has outstanding advantages in accurate and sensitive detection of low-level proteins due to its wide linear range, high sensitivity, and controllability [1][2][3]. Thus, it has been widely used in many fields, including clinical diagnosis [4], food safety [5], drug detection [6], and environmental monitoring [7]. Currently, ECL detection of proteins is usually accomplished by labelbased methods.…”