Novel biocatalysts for glycerol conversion into 2,3-butanediol

Ripoll, Vanessa; Vicente, G.; Morán, Bruno; Rojas, Antonia; Segarra, Silvia; Montesinos, Alejandro; Tortajada, Marta; Ramón, Daniel; Ladero, Miguel; Santos, Victoria E.

doi:10.1016/j.procbio.2016.03.006

Cited by 28 publications

(24 citation statements)

References 33 publications

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“…A batch-fermentation assay in 1.5-L bioreactor was performed with the wild-type strain at the optimal conditions determined in the variable optimization assay. All the glycerol was consumed during the cultivation, and a 2,3-BD concentration of 22 g L −1 was obtained, with a yield of 0.34 gram of 2,3-BD per gram of glycerol, which is 55% of the theoretical yield, 0.62 g of 2,3-BD per gram of glycerol obtained from an electron balance [8].…”

Section: Optimization Of Fermentation Conditions For the Wild-type Stmentioning

confidence: 95%

“…The strain Raoultella planticola CECT 843 was identified as one of the best producers of 2,3-BD from glycerol in a previous report [8]. 2,3-BD biosynthesis plays a very important physiological role in microorganisms preventing acidification, regulating NADH/NAD + , and storing carbon and energy for growth.…”

Section: Optimization Of Fermentation Conditions For the Wild-type Stmentioning

confidence: 96%

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Improved Raoultella planticola Strains for the Production of 2,3-Butanediol from Glycerol

et al. 2019

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Raw glycerol is an industrial byproduct from biodiesel production and is one of the most promising substrates for 2,3-butanediol (2,3-BD) production; however, 2,3-BD is not yet produced by fermentation from glycerol on a commercial scale due to poor process economics. Class 1 microorganism collections were screened and Raoultella planticola strain CECT 843 proved to be the best 2,3-BD producer, achieving (23.3 ± 1.4) g 2,3-BD per L and a yield of 36% (g 2,3-BD per g glycerol). To further increase product concentration and yield, R. planticola CEC T843 was subjected to random mutagenesis using ultra-violet (UV) light and ethyl methane sulfonate (EMS). Two mutant strains were found to produce at least 30% more 2,3-BD than the wild type: R. planticola IA1 [(30.8 ± 3.9) g 2,3-BD per L and 49% yield] and R. planticola IIIA3 [(30.5 ± 0.4) g 2,3-BD per L and 49% yield].

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Section: Optimization Of Fermentation Conditions For the Wild-type Stmentioning

confidence: 95%

Section: Optimization Of Fermentation Conditions For the Wild-type Stmentioning

confidence: 96%

Improved Raoultella planticola Strains for the Production of 2,3-Butanediol from Glycerol

et al. 2019

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“…25 O produto é tradicionalmente obtido por via química, a qual ocorre por meio dos derivados do petróleo, como a acroleína e o óxido de etileno, em reações como hidrogenação e hidroformilação, com formação de intermediários tóxicos. 26 Além da rota química, uma alternativa para a produção do 1,3-propanodiol que vem se destacando é a rota biológica, sendo o principal parâ-metro que influencia a cinética na produção de 1,3-propanodiol e a baixa concentração de biomassa, possuindo a literatura descrições de muitas alternativas de meios de cultivo que não tenham o glicerol como única fonte de carbono, aumentando a concentração de biomassa bacteriana.…”

Section: O 13-propanodiolunclassified

“…Esse alto rendimento, associado ao rápido crescimento em meios de cultivo simples, tem apontado o gênero K. oxytoca como de grande potencial biotecnológico. 26,35,37 Além da espécie oxytoca, até o momento K. pneumoniae e P. polymyxa têm sido as mais empregadas na produção eficiente de 2,3-BD. Aerogenes enterobacter e Serratia marcescens também são considerados potenciais micro-organismos, no entanto, as pesquisas com estes são menos relatadas na literatura.…”

Section: Condições Operacionais E Variáveis Envolvidas Na Produção Dounclassified

Produção biotecnológica de produtos de valor agregado utilizando glicerol residual proveniente da síntese de biodiesel

Silva

Souza

Antero

2017

evid.

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ResumoO aumento significativo na produção de biodiesel por transesterificação de óleos comestíveis e não comestíveis causaram excedente de glicerol no mercado. Com suas características, estrutura química única, renovabilidade e biodisponibilidade, o glicerol tem promissor potencial para ser transformado em produtos químicos de alto valor agregado. Este artigo oferece uma análise abrangente e crítica do tratamento biotecnológico do glicerol a 1,3-propanodiol e 2,3-butanodiol. A conversão microbiana do glicerol apresenta vantagens em relação às sínteses químicas requerendo menor número de etapas, temperaturas medianas e pressão do ambiente, além de não gerar intermediários tóxicos.Palavras-chave: Micro-organismos. Biodiesel. Transesterificação. Fermentação de glicerol.

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“…28,29 Recently, Raoultella terrigena has been proposed as biocatalyst to produce BD from glycerol. 30 The DO concentration is considered one of the most important variables affecting bioprocess performance, due to its influence on the key enzymes of the pathway and, hence, over the cell metabolic flux. 23,25 At low DO concentrations, glycerol is converted into 3-hydroxypropionaldehyde through glycerol dehydratase, and subsequently reduced to 1,3-propanediol by a dehydrogenase; both enzyme activities are regulated by coenzyme B12 and NADH, respectively.…”

Section: Introductionmentioning

confidence: 99%

Effect of fluid dynamic conditions on 2,3‐butanediol production by Raoultella terrigena in SBTR: oxygen transfer and uptake rates

Rodríguez

Ripoll

Santos

et al. 2016

J of Chemical Tech & Biotech

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BACKGROUND The fluid dynamic conditions play a key role in the development and scaling‐up of bioprocesses. In aerobic cultures, oxygen is an essential substrate for microbial growth, production and culture maintenance; an effective gas–liquid transfer must be achieved. Changes in fluid dynamics due to stirrer speed can affect the culture negatively, causing hydrodynamic stress (increasing shear stress) or oxidative stress (by an increase of available oxygen in the liquid phase). RESULTS Under oxygen‐limiting conditions, specific growth rate increases with stirrer speed, and several fermentation products were specifically released to the culture medium. 2,3‐butanediol production increased with stirrer speed, reaching a maximum at 400 rpm. When the agitation was increased over 550 rpm, the metabolic flux was mainly routed to increase the cell growth. Negative effects of fluid dynamic conditions on biomass production were observed at 1900 and 2000 rpm. Cellular response to shear stress conditions was also shown in the large increase of the broth viscosity over time. CONCLUSIONS Raoultella terrigena is able to adapt the carbon metabolic flux to the availability of oxygen, producing fermentation products, alcohols or directing microbial growth. Moreover, cells can withstand aggressive agitation conditions (up to 1600 rpm). © 2016 Society of Chemical Industry

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Novel biocatalysts for glycerol conversion into 2,3-butanediol

Cited by 28 publications

References 33 publications

Improved Raoultella planticola Strains for the Production of 2,3-Butanediol from Glycerol

Improved Raoultella planticola Strains for the Production of 2,3-Butanediol from Glycerol

Produção biotecnológica de produtos de valor agregado utilizando glicerol residual proveniente da síntese de biodiesel

Effect of fluid dynamic conditions on 2,3‐butanediol production by Raoultella terrigena in SBTR: oxygen transfer and uptake rates

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