Novel biodegradable electrospun membrane: scaffold for tissue engineering

Bhattarai, Shanta Raj; Bhattarai, Narayan; Yi, Ho Keun; Hwang, Pyong Han; Il, Dong; Kim, Hee Young

doi:10.1016/j.biomaterials.2003.09.043

Cited by 446 publications

(287 citation statements)

References 32 publications

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“…This high porosity allows the efficient exchange of nutrients and metabolic waste between the scaffold and its environment, and provides a high surface area for local and sustained delivery of biochemical signals to the seeded cells [11][12][13][14][15][16]. On the other hand, the small pore size of reported electrospun scaffolds has thus far shown to be difficult for ingrowth and migration of seeded cells.…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, the small pore size of reported electrospun scaffolds has thus far shown to be difficult for ingrowth and migration of seeded cells. To date, electrospinning has been used for the fabrication of scaffolds from numerous biodegradable polymers, such as poly(ε-caprolactone) (PCL), poly(lactic acid) (PLA), poly(glycolic acid) (PGA), poly(lactide-co-glycolide) (PLGA) and polyurethane (PU); natural proteins have been used as well including collagen, elastin and gelatin [11,13,[17][18][19][20][21][22][23]. Gelatin is a natural protein derived from collagen by controlled hydrolysis and is a heterogeneous mixture of single-or multistranded polypeptides containing between 300 -4000 amino acids.…”

Section: Introductionmentioning

confidence: 99%

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Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

et al. 2008

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Electrospinning using natural proteins or synthetic polymers is a promising technique for the fabrication of fibrous scaffolds for various tissue engineering applications. However, one limitation of scaffolds electrospun from natural proteins is the need to cross-link with glutaraldehyde for stability, which has been postulated to lead to many complications in vivo including graft failure. In this study, we determined the characteristics of hybrid scaffolds composed of natural proteins including collagen and elastin, as well as gelatin, and the synthetic polymer poly(ε-caprolactone) (PCL), so to avoid chemical cross-linking. Fiber size increased proportionally with increasing protein and polymer concentrations, whereas pore size decreased. Electrospun gelatin/PCL scaffolds showed a higher tensile strength when compared to collagen/elastin/PCL constructs. To determine the effects of pore size on cell attachment and migration, both hybrid scaffolds were seeded with adipose-derived stem cells. Scanning electron microscopy and nuclei staining of cell-seeded scaffolds demonstrated complete cell attachment to the surfaces of both hybrid scaffolds, although cell migration into the scaffold was predominantly seen in the gelatin/PCL hybrid. The combination of natural proteins and synthetic polymers to create electrospun fibrous structures resulted in scaffolds with favorable mechanical and biological properties.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

et al. 2008

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“…the straight portion of the jet increased and the looping cone decreased with the voltage. However, increased salt concentration (and hence solution conductivity) only slightly decreased the cone angle θ for two salts NaCl and NH 4 Cl, whereas the opposite behavior was observed for LiCl. The jet straight part L increased with the salt concentration only for NaCl, whereas it decreased for the two other salts.…”

Section: Methodsmentioning

confidence: 79%

“…Such fibers find applications in a variety of areas, including wound dressing [4], drug or gene delivery vehicles [19], biosensors [16], fuel cell membranes and electronics [15]. Recently electrospinning has been revitalized and successfully applied to the production of nanofibrous scaffolds for tissue-engineering processes [2], which constitute one of its most promising application.…”

Section: Motivationmentioning

confidence: 99%

Modeling Electrospinning of Nanofibers

Kowalewski

Barral

Kowalczyk

IUTAM Symposium on Modelling Nanomaterials and Nanosystems

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A fast discrete model for the simulations of thin charged jets produced during the electrospinning process is derived, based on an efficient implementation of the boundary element method for the computation of electrostatic interactions of the jet with itself and with the electrodes. Short-range electrostatic forces are evaluated with slender-body analytical approximations, whereas a hierarchical force evaluation algorithm is used for long-range interactions. Qualitative comparisons with experiments is discussed. MotivationElectrospinning is a simple and relatively inexpensive mean of producing nanofibers by solidification of a polymer solution stretched by an electric field. Such fibers find applications in a variety of areas, including wound dressing [4], drug or gene delivery vehicles [19], biosensors [16], fuel cell membranes and electronics [15]. Recently electrospinning has been revitalized and successfully applied to the production of nanofibrous scaffolds for tissue-engineering processes [2], which constitute one of its most promising application.A conventional electrospinning setup consists of a spinneret with a metallic needle, a syringe pump, a high-voltage power supply and a grounded collector. A polymer solution is loaded into the syringe and driven through the needle at a steady and controllable feed rate by the pump, forming a droplet at the tip of the needle. A high voltage (typically up to 30 kV) is applied between the tip and a grounded collector. When the electric field strength overcomes the surface tension of the droplet an electrified liquid jet is formed. The jet is then elongated and whipped continuously by electrostatic repulsion (bending instability), describing a chaotic spiraling motion on its way to the collecting electrode. Although the process may appear

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“…The aligned nano fibres can be potentially applied in composite materials, reinforcements, electrochemical sensing, bone and blood vessel engineering and tissue engineering. [4][5][6] The electrospun nano fibres have very large surface area to volume ratio and porous structure as compared to the conventional fibre. Due to this reason these fibres can be used in adsorption of chemicals, military and civilian filtration.…”

Section: Introductionmentioning

confidence: 99%

Nano Fibres by Electro spinning: Properties and Applications

Nayak¹,

Padhye²

2017

JTEFT

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Electro spinning is a simple and versatile technique applicable to a wide range of polymers and solvents for the production of materials in nanometer scale. The nano fibrous materials produced by electro spinning process exhibit novel and significantly improved physical, chemical and biological properties. The small fibre diameter and the porous structure of the electrospun surfaces enable them for a wide range of applications such as wound dressing, tissue engineering scaffolds, vascular grafts, filters, membranes and sensors. In this article the principle, setup, materials used and the factors affecting the electro spinning process have been described. Also the properties & applications of electrospun fibres and the limitations & future scope of electro spinning process have been highlighted.

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Novel biodegradable electrospun membrane: scaffold for tissue engineering

Cited by 446 publications

References 32 publications

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

Modeling Electrospinning of Nanofibers

Nano Fibres by Electro spinning: Properties and Applications

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