Novel biomarkers of bladder decompensation after partial bladder obstruction

Abstract

This study shows that prolonged PBOO causes progressive deterioration in the rabbit bladder with decompensation after 8 weeks. Markedly decreased nerve density and severely reduced SOD and CAT activities are associated with the shift from compensated to decompensated function of the bladder. They may be excellent biomarkers of decompensation.

Partial bladder outlet obstruction reduces the tissue antioxidant capacity and muscle nerve density of the guinea pig bladder

“…18 A recent study demonstrated that the endogenous antioxidant enzymes decrease in obstructed bladder tissue. 19 Our study showed that the antioxidant capacity, as measured by the TEAC test, significantly decreased in obstructed bladders compared to sham operated bladders. This was partly due to a reduced amount of glutathione (GSH) whereas uric acid did not decrease significantly (Fig.…”

“…This is in accordance with the results of a recent study which reports that the activity of the antioxidant enzymes superoxide dismutase and catalase were reduced in bladder tissue exposed to an outlet obstruction. 19 The fact that the endogenous antioxidant system has been deteriorated suggests that obstructed bladders are exposed to elevated levels of RNOS.…”

Protective effect of sulfhydryl‐containing antioxidants against ischemia/reperfusion injury of prepubertal rat intestine

“…1,10,11 ROS are normally removed by antioxidant enzymes; superoxide dismutase (SOD) rapidly and specifically reduces superoxide to H2O2, and glutathione peroxidase (GPx) degrades H2O2 to water. 12,13 The potent ability of ROS to induce injury in the intestine has been established. 4,5,14,15 Therefore, scavenging of free radicals by antioxidants might have an important role in the prevention of I/R injury.…”

Calcium Effects on Superoxide Dismutase and Catalase of the Rabbit Urinary Bladder Muscle and Mucosa

“…Bladder microsomes (synaptic membranes and SR) and mitochondria are especially sensitive to ischemic damage [46,47] . This occurs during partial outlet obstruction in rabbits and men [24,25,48] , and after ovariectomy in female rabbits [26,46] . SOD and catalase play a major role in the protection of intracellular organelles, especially mitochondria, against free radical damage, and thus a decrease in their activity would increase the cells' sensitivity to ischemic damage.…”

“…This increase in thickness creates a severe hypoxic condition when the bladder contracts and a reperfusion condition (free radical generation) when the bladder relaxes. The result is a progressive increase in oxidative damage associated with increasing severity of obstructive bladder dysfunction [14,15,[23][24][25] .…”