Novel Carbapenemases FLC-1 and IMI-2 Encoded by an Enterobacter cloacae Complex Isolated from Food Products

Brouwer, Michael S.M.; Tehrani, Kamaleddin Haj Mohammad Ebrahim; Rapallini, Michel; Geurts, Yvon; Kant, A.; Harders, Frank; Mashayekhi, Vida; Martin, Nathaniel I.; Bossers, Alex; Mevius, Dik; Wit, Ben; Veldman, Kees

doi:10.1128/aac.02338-18

Cited by 21 publications

(20 citation statements)

References 24 publications

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“…Enterobacter cloacae (E. cloacae) is a common gram-negative, rod-shaped bacterium belonging to the family Enterobacteriaceae and can be frequently detected from human and animal excrement. E. cloacae has also been isolated from food processing plants, rice, seafoods and meat products, which can bring out food spoilage [1,2]. Consequently, E. cloacae has recently been used as a hygiene indicator in foodstuff processing, and is also one of the most challenging bacterial contaminants of raw and processed meat products, and vegetables [3].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: In Vitro Antibacterial Activity and Mechanism of Vanillic Acid against Carbapenem-Resistant Enterobacter cloacae

Qian

Liu

et al. 2019

Antibiotics

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Vanillic acid (VA) is a flavoring agent found in edible plants and fruits. Few recent studies exhibited robust antibacterial activity of VA against several pathogen microorganisms. However, little was reported about the effect of VA on carbapenem-resistant Enterobacter cloacae (CREC). The purpose of the current study was to assess in vitro antimicrobial and antibiofilm activities of VA against CREC. Here, minimum inhibitory concentrations (MIC) of VA against CREC was determined via gradient diffusion method. Furthermore, the antibacterial mode of VA against CREC was elucidated by measuring changes in intracellular adenosine triphosphate (ATP) concentration, intracellular pH (pHin), cell membrane potential and membrane integrity. In addition, antibiofilm formation of VA was measured by crystal violet assay and visualized with field emission scanning electron microscopy (FESEM) and confocal laser scanning microscopy (CLSM). The results showed that MIC of VA against E. cloacae was 600 μg/mL. VA was capable of inhibiting the growth of CREC and destroying the cell membrane integrity of CREC, as confirmed by the decrease of intracellular ATP concentration, pHin and membrane potential as well as distinctive variation in cellular morphology. Moreover, crystal violet staining, FESEM and CLSM results indicated that VA displayed robust inhibitory effects on biofilm formation of CREC and inactivated biofilm-related CREC cells. These findings revealed that VA exhibits potent antibacterial activity against CREC, and thus has potential to be exploited as a natural preservative to control the CREC associated infections.

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Section: Introductionmentioning

confidence: 99%

RETRACTED: In Vitro Antibacterial Activity and Mechanism of Vanillic Acid against Carbapenem-Resistant Enterobacter cloacae

Qian

Liu

et al. 2019

Antibiotics

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“…IMI-2 was firstly identified in E. asburiae recovered from environmental samples in US rivers from 1999 to 2001 ( 18 ). It has been identified in Klebsiella variicola in UK, in E. asburiae in Czech Republic, in E. cloacae from Spanish rivers, France, and Canada, Enterobacter mori in Austria, and E. coli in Spain and China ( 19 – 24 ). IMI-3 was firstly detected in China and France ( 21 , 25 , 26 ).…”

Section: Minor Class a Carbapenemasesmentioning

confidence: 99%

Genetic Diversity, Biochemical Properties, and Detection Methods of Minor Carbapenemases in Enterobacterales

et al. 2021

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Gram-negative bacteria, especially Enterobacterales, have emerged as major players in antimicrobial resistance worldwide. Resistance may affect all major classes of anti-gram-negative agents, becoming multidrug resistant or even pan-drug resistant. Currently, β-lactamase-mediated resistance does not spare even the most powerful β-lactams (carbapenems), whose activity is challenged by carbapenemases. The dissemination of carbapenemases-encoding genes among Enterobacterales is a matter of concern, given the importance of carbapenems to treat nosocomial infections. Based on their amino acid sequences, carbapenemases are grouped into three major classes. Classes A and D use an active-site serine to catalyze hydrolysis, while class B (MBLs) require one or two zinc ions for their activity. The most important and clinically relevant carbapenemases are KPC, IMP/VIM/NDM, and OXA-48. However, several carbapenemases belonging to the different classes are less frequently detected. They correspond to class A (SME-, Nmc-A/IMI-, SFC-, GES-, BIC-like…), to class B (GIM, TMB, LMB…), class C (CMY-10 and ACT-28), and to class D (OXA-372). This review will address the genetic diversity, biochemical properties, and detection methods of minor acquired carbapenemases in Enterobacterales.

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“…7 According to their active sites, carbapenemases are classified into two main classes: serine carbapenemases and metallo-carbapenemases. 8,9 Serine carbapenemases are further classified into two molecular groups according to the Ambler classification of β-lactamases: class A serine carbapenemases (CASCs) and class D serine carbapenemases, which are also known as OXA-type carbapenemases (OTCs) in which these carbapenemases are actually oxacillinases. 10 In clinical settings, CASCs can be inhibited by βlactamase inhibitors such as clavulanic acid, sulbactam, and tazobactam.…”

Section: Introductionmentioning

confidence: 99%

“…Representative DNA fingerprint pattern generated by BOX-PCR for carbapenem-resistant K. pneumoniae clinical isolates. L, 100 bp DNA ladder; lanes 3, 4,5,6,7,8,9,10,11,12,13,14,15,16,17, and 18 are the code No. of CRKP clinical isolates.…”

mentioning

confidence: 99%

The First Egyptian Report Showing the Co-Existence of blaNDM-25, blaOXA-23, blaOXA-181, and blaGES-1 Among Carbapenem-Resistant K. pneumoniae Clinical Isolates Genotyped by BOX-PCR

El-Badawy¹,

El‐Far²,

Althobaiti³

et al. 2020

IDR

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Background and Objective: The emergence of carbapenem-resistant K. pneumoniae (CRKP) continues to escalate and is alarming because of the emergence of pan drugresistant strains. The objective of this study was to investigate the existence of 12 carbapenemase genes among CRKP clinical isolates. Methods: Ninety-six Klebsiella spp. clinical isolates were collected. The isolates were identified phenotypically and genotypically. These isolates were screened for susceptibility to 24 different antibiotics. The modified Hodge test (MHT) and the Carba Nordmann/Poirel (NP) test were used to phenotypically screen carbapenem-resistant strains for carbapenemase production. Phenotypic characterization of carbapenemases was performed using the combined disk synergy test (CDST). Additionally, the presence of 12 carbapenemase genes in CRKP isolates was investigated. The DNA sequence of bla NDM and bla GES genes was determined. The BOX-PCR technique was used to determine the clonal relationship between CRKP isolates. Results: All carbapenem-resistant isolates were related to K. pneumoniae. Susceptibility testing showed that 19.79% (19/96) of the collected isolates were carbapenem-resistant. Of the CRKP isolates, 68.42% (13/19) tested positive for the MHT and Carba NP test. CDST showed that 42.11% (8/19), 63.16% (12/19), 47.37% (9/19), and 73.68% (14/19) of the CRKP isolates tested positive for the inhibitory effect of clavulanic acid, sulbactam, phenylboronic acid, and tazobactam, respectively, while 84.21% (16/19) and 68.42% (13/16) tested positive for the inhibitory effect of EDTA and mercaptopropionic acid, respectively. It was found that 10.53% (2/19) of the isolates tested positive for the inhibitory effect of sodium chloride. Molecular investigation of carbapenemases showed that 26.32% (5/19), 73.68% (14/19), 21.05% (4/19), 10.53% (2/19), and 5.26% (1/19) of the isolates tested positive for bla NDM , bla OXA-48 , bla OXA-181 , bla OXA-51 , and bla OXA-23 , respectively. None of the isolates tested positive for bla OXA-40 and bla OXA-58. Two allelic variants of bla NDM (bla NDM-1 and bla NDM-25) were detected. BOX-PCR revealed high clonal relatedness between CRKP isolates. Conclusion: MHT was more sensitive than Carba NP test for evaluating carbapenemase production and class D carbapenemase genes were the most prevalent of the 12 carbapenemase genes that were evaluated.

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Novel Carbapenemases FLC-1 and IMI-2 Encoded by an Enterobacter cloacae Complex Isolated from Food Products

Cited by 21 publications

References 24 publications

RETRACTED: In Vitro Antibacterial Activity and Mechanism of Vanillic Acid against Carbapenem-Resistant Enterobacter cloacae

RETRACTED: In Vitro Antibacterial Activity and Mechanism of Vanillic Acid against Carbapenem-Resistant Enterobacter cloacae

Genetic Diversity, Biochemical Properties, and Detection Methods of Minor Carbapenemases in Enterobacterales

<p>The First Egyptian Report Showing the Co-Existence of <em>bla</em><sub>NDM-25</sub>, <em>bla</em><sub>OXA-23</sub>, <em>bla</em><sub>OXA-181</sub>, and <em>bla</em><sub>GES-1</sub> Among Carbapenem-Resistant <em>K. pneumoniae</em> Clinical Isolates Genotyped by BOX-PCR</p>

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