Novel CBFB-MYH11 fusion transcripts or reverse transcription-polymerase chain reaction artifacts? [letter; comment]

Therapy-related myelodysplastic syndrome with inv(16)(p13q22) and I type CBFβ/MYH11 after autologous transplantation: Undetectable fusion transcript in pretransplant progenitor cells

“…Amplification of MYH11 intronic sequences in the present study could be explained similarly. Other PCR artifacts due to aspecific annealing of MYH11 M1 primer to another positionof MYH11 cDNA were also reported[20]. We have shown here that the nested RT-PCR method described by van der Reijden et al[15] using cd-mm and cmd1-mmd2 primer sets could also amplify PCR artifacts such as CBFβ cDNA and MYH11 genomic DNA, resulting in false positive results.…”