Novel chitosan/collagen scaffold containing transforming growth factor-β1 DNA for periodontal tissue engineering

Zhang, Yufeng; Cheng, Xiang‐Rong; Wang, Jiawei; Shu, Bin; Huang, Cui; Yang, Xuechao; Liu, Tongjun

doi:10.1016/j.bbrc.2006.03.106

Cited by 147 publications

(79 citation statements)

References 31 publications

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“…For the proliferation assay, methyl thiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) was used as previously described [11,12].…”

Section: Proliferation Assaymentioning

confidence: 99%

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Effects of Fluoride-Releasing Restorative Materials on Cultured Human Periodontal Ligament Fibroblast Cell Behavior

Phunturug¹,

Kesrak

2014

LSMR

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Abstract-Fluoride-releasing materials are commonly used to restore cervical lesions and in reconstructive periodontal surgery. The attachment and proliferation of various materials on human periodontal ligament fibroblast (HPDL) cells were evaluated in this study. Four fluoride-releasing restorative materials were tested: GIC (F9), RMGIC (F2), Giomer (B), and resin composite (G); while resin composite (P) was used as a negative control. The specimens were prepared according to the standard protocol, and then primary cultures of HPDL cells were seeded on specimens and control glass cover slips. The attached cells were counted at 1, 3, 24, and 72 h after cell seeding. Cell proliferation was determined using MTT assay at 1, 3, 5, and 7 days after cell seeding. The cell morphology was determined by SEM. Cell attachment increased as time elapsed for all materials. After initial attachment, Phad the best cell attachment profile when compared to the other groups. G had the greatest amount of cell attachment at the end of cell culture time, while F9 showed the least. The cell proliferation profile of G was the highest, while F9 and F2 were the least (p < 0.05) among all groups. SEM evaluation determined that HPDL cells on the materials were round or oval at the initial time of cell culture. As time elapsed, HPDL cells presented a variety of cell morphologies in the development of cytoplasmic processes.

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“…For the proliferation assay, methyl thiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) was used as previously described [11,12].…”

Section: Proliferation Assaymentioning

confidence: 99%

“…A polystyrene 96-well tissue culture plate was used as a positive control (C) group. The process was performed as previously described by Zhang, et al [11] and Shin, et al [12].…”

Section: Proliferation Assaymentioning

confidence: 99%

Effects of Fluoride-Releasing Restorative Materials on Cultured Human Periodontal Ligament Fibroblast Cell Behavior

Phunturug¹,

Kesrak

2014

LSMR

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“…Recent studies have illustrated that exogenous TGF-β 1 had a strong effect on the collagen production of ACL cells [7]. Meanwhile, TGF-β 1 expression could be regulated by transferring the TGF-β 1 gene into ACL cells to enhance the collagen type synthesis in vitro [8]. Furthermore, TGF-β 1 could promote fibronectin synthesis, which plays an important role in the complicated interaction between ligament cells and their matrix environment [9].…”

Section: Introductionmentioning

confidence: 99%

Up-regulation expressions of lysyl oxidase family in Anterior Cruciate Ligament and Medial Collateral Ligament fibroblasts induced by Transforming Growth Factor-Beta 1

Xie

Jiang

Zhang

et al. 2011

International Orthopaedics (SICOT)

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Purpose The lysyl oxidase (LOX) family plays a crucial role in the formation and stabilisation of extracellular matrix (ECM) by catalysing the cross-linking of collagen and elastin, implicating its important fundamental roles in injury healing. A high level of transforming growth factor-β 1 (TGF-β 1 ) accompanies the inflammatory phase of an injury of the knee joint. Our purpose was to detect the expressions of the LOX family in anterior cruciate ligament (ACL) and medial collateral ligament (MCL) response to TGF-β 1 . Methods This study used reversed transcript PCR, real time quantitative PCR and Western blot for analyses. Results The results showed significant increases in mRNA levels of LOX family members. At 5 ng/ml concentration of TGF-β 1,

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“…The following criteria for the scaffolds were selected based on the design considerations for our cell seeding method: (a) Thickness less than 500 µm [13]; (b) Pore size greater than 50 µm; (c) Porosity greater than 50% [12,14].…”

Section: Scaffold Optimizationmentioning

confidence: 99%

Design of a Novel Method for the Spatial Distribution of Cells within a Porous Scaffold for Tissue Engineering Applications

Subramanian¹,

Bhowmick²,

Gappa-Fahlenkamp³

2017

J Tissue Sci Eng

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Tissue engineering is rapidly progressing to provide complex, three-dimensional (3D) representations of human tissues that can be used for tissue replacement and/or to study tissue systems. Tissue engineering includes the addition of cells within 3D scaffolds, along with bioactive components, sometimes within a bioreactor. A major challenge in developing many tissue-engineered models is the ability to evenly distribute cells throughout a porous scaffold, in order to achieve good cell viability and growth. In this study, we created a 3D collagen-chitosan scaffold with specific properties to aid in seeding cells within the entire volume and investigated a dynamic method to seed cells within such scaffold. Based on the requirements for cell seeding, the scaffolds were less than 500 µm thick, had pore sizes greater than 50 µm and had a porosity of 50% or greater. Fibroblasts were used as model cells for this seeding method. To seed fibroblasts within the scaffold, we varied two design parameters: concentration of the collagen seeding solution and the centrifugal force used for cell seeding. We ranked the seeding efficiency, cell proliferation and distribution in order to choose the ideal cell seeding method. Results showed that seeding with a higher concentration (2 mg/ml) of collagen seeding solution and a lower centrifugation speed (259 ×g) was the optimal seeding method, resulting in 84% increase in cell proliferation and a more uniform cell distribution throughout the scaffold. Results from this study can be applied for seeding a variety of cell populations within porous scaffolds for tissue engineering applications.

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Novel chitosan/collagen scaffold containing transforming growth factor-β1 DNA for periodontal tissue engineering

Cited by 147 publications

References 31 publications

Effects of Fluoride-Releasing Restorative Materials on Cultured Human Periodontal Ligament Fibroblast Cell Behavior

Effects of Fluoride-Releasing Restorative Materials on Cultured Human Periodontal Ligament Fibroblast Cell Behavior

Up-regulation expressions of lysyl oxidase family in Anterior Cruciate Ligament and Medial Collateral Ligament fibroblasts induced by Transforming Growth Factor-Beta 1

Design of a Novel Method for the Spatial Distribution of Cells within a Porous Scaffold for Tissue Engineering Applications

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