Novel Complex Class 1 Integron Bearing an IS
 CR1
 Element in an
 Escherichia coli
 Isolate Carrying the
 bla
 CTX-M-14
 Gene

Bae, Il Kwon; Lee, You-Nae; Lee, Wee Gyo; Lee, Sangeun; Jeong, Seok Hoon

doi:10.1128/aac.00279-07

Cited by 33 publications

(28 citation statements)

References 18 publications

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“…Indeed, the insertion probably occurred at the recombination crossover site (ACCC-) at the 3Ј end of ISCR1 (Fig. 2), as described previously (1,48).…”

Section: Resultssupporting

confidence: 67%

“…70 kb containing qnrA3 were transferred at a [50]; pQR1 [26]; pQKp331H [25]) and pSAL-1, containing DHA-1, the AmpC-type ␤-lactamase gene originating from chromosomal DNA of Morganella morganii (48). RCS, recombination crossover site (1). Sequences that were found in at least two plasmids are shown as motifs (M) with the number of base pairs: M233 (sequence under GenBank accession no.…”

Section: Resultsmentioning

confidence: 99%

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A Plasmid-Borne Shewanella algae Gene, qnrA3 , and Its Possible Transfer In Vivo between Kluyvera ascorbata and Klebsiella pneumoniae

et al. 2008

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The plasmid-borne quinolone resistance gene qnrA1 is prevalent in multidrug-resistant Enterobacteriaceae. A chromosomally encoded homologue in Shewanella algae, qnrA3, has been described. We isolated two qnrA3-positive strains, one of Klebsiella pneumoniae (He96) and one of Kluyvera ascorbata (Kas96), from the feces of an immunocompromised outpatient. The qnrA3 allele was identical to that of S. algae except for 5 nucleotides and differed from qnrA1 by 29 nucleotides affecting three amino acids. The analysis of the qnrA3 genetic environment showed that qnrA3 was inserted downstream from an ISCR1 element at a recombination crossover site described for other resistance genes, including qnrA1, and immediately upstream from IS26, a situation not described before. IS26 preceded an incomplete class 1 integron which contained, among other genes, aac(6)-Ib-cr, another transferable quinolone resistance gene, and the ␤-lactamase gene bla OXA-1/30 . The 10-kb fragment encompassing qnrA3 was compared to previously described qnrA1-containing plasmids and multidrugresistant plasmids; it shares identical sequences with pC15a, pHSH2, pQR1, pQKp311H, and pSAL-1 but with rearrangements, deletions, and mutations. Conjugal transfer of qnrA3 was highly efficient (10 ؊2 ) from K. pneumoniae He96 or K. ascorbata Kas96 to Escherichia coli J53 but less so (10 ؊5 ) from either donor to a clinical strain of Enterobacter cloacae. This first description of a plasmid-borne copy and of the in vitro transfer of qnrA3 is taken to illustrate its likely in vivo transfer from S. algae to the Enterobacteriaceae.

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“…Indeed, the insertion probably occurred at the recombination crossover site (ACCC-) at the 3Ј end of ISCR1 (Fig. 2), as described previously (1,48).…”

Section: Resultssupporting

confidence: 67%

Section: Resultsmentioning

confidence: 99%

A Plasmid-Borne Shewanella algae Gene, qnrA3 , and Its Possible Transfer In Vivo between Kluyvera ascorbata and Klebsiella pneumoniae

et al. 2008

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“…Furthermore, those analyses confirmed the predominant role of ISEcp1 in the mobilization of bla CTX-M genes of the CTX-M-1 group (of human isolates) and the presence of a novel complex class 1 integron (18). The CTX-M-14 enzyme of a clinical E. coli isolate and the CTX-M-9 enzyme of a Salmonella isolate from healthy food animals were also part of a complex class 1 integron (3,37). Further analysis to determine the genetic environment of these genes is needed to confirm whether the CTX-M enzymes found in our study are also located in similar structures.…”

Section: Discussionmentioning

confidence: 65%

Diversity of Extended-Spectrum β-Lactamases and Class C β-Lactamases among Cloacal Escherichia coli Isolates in Belgian Broiler Farms

Smet

Martel

Persoons

et al. 2008

Antimicrob Agents Chemother

199

195

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A total of 295 ceftiofur-resistant Escherichia coli isolates were obtained from 489 cloacal samples collected at five different Belgian broiler farms with the aim to evaluate the diversity of this resistance at the farm level. Strains were examined for resistance against ␤-lactam antibiotics and other antimicrobial agents by using disk diffusion tests. Three different ␤-lactam resistance phenotypes suggested the presence of an extended-spectrum ␤-lactamase (ESBL), a class C ␤-lactamase, or the combination of an ESBL with a class C ␤-lactamase. Seventy-six percent of these isolates also showed acquired resistance to other antimicrobial agents. After genotyping by repetitive extragenic palindromic-PCR, 51 unrelated E. coli strains were selected for further analyses. Isoelectric focusing and sequencing of the amplicons obtained in PCRs for the detection of genes encoding broad-spectrum ␤-lactamase enzymes revealed the following ESBLs: TEM-52 (13.2%), TEM-106 (2%), CTX-M-1 (27.4%), CTX-M-2 (7.8%), CTX-M-14 (5.9%), and CTX-M-15 (2%). The only plasmidic AmpC ␤-lactamase found in this study was the CMY-2 enzyme (49%). Mutations in the promoter and attenuator regions of the chromosomal ampC gene were found only in association with bla CMY-2 genes and ESBL genes. The combination of an ESBL (CTX-M-1) with a plasmidic AmpC ␤-lactamase (CMY-2) was found in 7.8% of the isolates. These data show that ceftiofur-resistant E. coli strains are often present in cloacal samples of broilers at the farm level in Belgium. The diversity of broad-spectrum ␤-lactamases among these isolates is high, and they may act as a reservoir of ESBL and ampC genes.

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“…As bla CTX-M-14 has been found to be associated with different genetic environments, we tested for the presence of sequences associated with known genetic platforms: ISEcp1, IS903, intI1, and ISCR1 (2,17,39). Oligonucleotides corresponding to different regions of ISEcp1 and IS903 were used in order to detect elements containing ⌬ISEcp1 and/or ⌬IS903, as described previously (17).…”

Section: Methodsmentioning

confidence: 99%

“…Analysis of specific isolates identified two variants of bla CTX-M-14 on genetic platforms containing either ISEcp1 or ISCR1 sequences, highlighting the different pathways for the acquisition and evolution of this gene (2,4,17,39). Hitherto, studies describing the occurrence of this ESBL have analyzed only a limited number of isolates at the molecular level.…”

mentioning

confidence: 99%

Spread of bla _CTX-M-14 Is Driven Mainly by IncK Plasmids Disseminated among Escherichia coli Phylogroups A, B1, and D in Spain

Valverde

Cantón

Garcillán-Barcia

et al. 2009

Antimicrob Agents Chemother

116

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Since their first description in the late 1980s, plasmid-mediated CTX-M extended-spectrum ␤-lactamases (ESBLs) have increasingly been reported worldwide in both hospital and community settings and have mainly been associated with Escherichia coli isolates, which often cause urinary tract infections (UTIs) (7,46,48,56). The CTX-M enzymes are classified into five groups on the basis of their amino acid sequences, and of these, the CTX-M-1 and CTX-M-9 clusters have been the most frequently reported worldwide (7).The CTX-M-14 enzyme is, besides CTX-M-9, the most widespread enzyme of the CTX-M-9 group. It was initially identified among Shigella clinical isolates from Korea in 1995 (45); and it is now globally disseminated, being endemic in Spain, France,

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Novel Complex Class 1 Integron Bearing an IS CR1 Element in an Escherichia coli Isolate Carrying the bla _CTX-M-14 Gene

Cited by 33 publications

References 18 publications

A Plasmid-Borne Shewanella algae Gene, qnrA3 , and Its Possible Transfer In Vivo between Kluyvera ascorbata and Klebsiella pneumoniae

A Plasmid-Borne Shewanella algae Gene, qnrA3 , and Its Possible Transfer In Vivo between Kluyvera ascorbata and Klebsiella pneumoniae

Diversity of Extended-Spectrum β-Lactamases and Class C β-Lactamases among Cloacal Escherichia coli Isolates in Belgian Broiler Farms

Spread of bla _CTX-M-14 Is Driven Mainly by IncK Plasmids Disseminated among Escherichia coli Phylogroups A, B1, and D in Spain

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Novel Complex Class 1 Integron Bearing an IS CR1 Element in an Escherichia coli Isolate Carrying the bla CTX-M-14 Gene

Cited by 33 publications

References 18 publications

A Plasmid-Borne Shewanella algae Gene, qnrA3 , and Its Possible Transfer In Vivo between Kluyvera ascorbata and Klebsiella pneumoniae

A Plasmid-Borne Shewanella algae Gene, qnrA3 , and Its Possible Transfer In Vivo between Kluyvera ascorbata and Klebsiella pneumoniae

Diversity of Extended-Spectrum β-Lactamases and Class C β-Lactamases among Cloacal Escherichia coli Isolates in Belgian Broiler Farms

Spread of bla CTX-M-14 Is Driven Mainly by IncK Plasmids Disseminated among Escherichia coli Phylogroups A, B1, and D in Spain

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About

Novel Complex Class 1 Integron Bearing an IS CR1 Element in an Escherichia coli Isolate Carrying the bla _CTX-M-14 Gene

Spread of bla _CTX-M-14 Is Driven Mainly by IncK Plasmids Disseminated among Escherichia coli Phylogroups A, B1, and D in Spain