Novel cross-linked polyacrylamide matrices: An investigation using gradient gel electrophoresis

Abstract

Gradient gel electrophoresis was used to examine the separation properties of novel cross-linking compounds for polyacrylamide (PAAm). At low %T and at the same %C protein migration difference is accentuated for bismethacrylamide cross-linked networks relative to bisacrylamide cross-linked networks. Similar properties were observed for cyclic monomers at low %T. This trend is maintained throughout the gradient. However, at higher %T migration differential relative to N,N'-methylenebisacrylamide (Bis) was less pronounced. Evidence from gradient gels suggests that reactivity and functionality of vinyl groups impose an overriding control over network formation.

Newly designed polyacrylamide/dextran gels for electrophoresis protein separation: synthesis and characterization

“…SDS‐PAGE) was performed using standard proteins with known molecular weights (Table 1). The protein separation performance can be rationalized by plotting the logarithm of molecular weight versus retardation factor R f defined as the ratio of migration distances of proteins and solvent 14, 31–33. The results are depicted in Fig.…”