2013
DOI: 10.1016/j.jbiotec.2013.02.003
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Novel Description of mcl-PHA Biosynthesis by Pseudomonas chlororaphis from Animal-Derived Waste

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Cited by 87 publications
(64 citation statements)
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“…Efforts in this direction are to an increasing extent devoted to the selection of inexpensive carbon feedstocks, which can replace costly raw materials typically used for biotechnological purposes, such as sugars or edible oils [11]. Among such inexpensive feedstocks, a number of [agro]industrial surplus materials was already investigated for PHA production, such as waste streams from dairy and cheese making industry [12,13], residues of the biodiesel and animal processing industry [14][15][16], or various abundant lignocellulosics [17], e.g., hydrolyzed bagasse and fruit peels [18], straw [19,20] or even spent coffee ground [21]. Further, novel processes to recover PHA from microbial biomass are currently in status of development, predominately aiming at the reduction of the input of chemicals and solvents, and at the conservation of the native polymer properties, hence, the PHA´s molecular mass and its quasi-amorphous state [22][23][24].…”
Section: Introductionmentioning
confidence: 99%
“…Efforts in this direction are to an increasing extent devoted to the selection of inexpensive carbon feedstocks, which can replace costly raw materials typically used for biotechnological purposes, such as sugars or edible oils [11]. Among such inexpensive feedstocks, a number of [agro]industrial surplus materials was already investigated for PHA production, such as waste streams from dairy and cheese making industry [12,13], residues of the biodiesel and animal processing industry [14][15][16], or various abundant lignocellulosics [17], e.g., hydrolyzed bagasse and fruit peels [18], straw [19,20] or even spent coffee ground [21]. Further, novel processes to recover PHA from microbial biomass are currently in status of development, predominately aiming at the reduction of the input of chemicals and solvents, and at the conservation of the native polymer properties, hence, the PHA´s molecular mass and its quasi-amorphous state [22][23][24].…”
Section: Introductionmentioning
confidence: 99%
“…The utilization of inexpensive substrates [36][37][38][39][40][41][42] , modeling 43 , proper experimental design 44,45 and development of new recovery method [46][47][48][49][50] as well as opportunities for their competition in the global market has recently been addressed. Besides various application of PHB 51 , its advantages and disadvantages in food packaging have been also reported 21 while little information can be found regarding PHB usage as food packaging.…”
Section: Phbmentioning
confidence: 99%
“…After tightly locking the tubes, the incubation was carried out for 3.5 h at 98°C in a thermostated water bath under continuous shaking. After cooling the tubes, 1 mL of aqueous NaHCO 3 solution was added (NaHCO 3 solution was used instead of pure water in order to protect the GC-column by lowering the acidity of the sample [15]); after intensive vortexing and subsequent centrifugation for phase separation, the organic phase was subjected towards analysis of 3HAMEs using a HP 5890 Series II gas chromatograph (GC; 30 m HP5 column, protected by a 5 m HP 1 capillary pre-column) according to the protocol described by previously [14]. 3HAMEs (methyl esters of PHA building blocks 3HB and 3HV) were registered by a flame ionization detector, the carrier gas helium was used with a split-ratio of 1:10.…”
Section: Pha Analysis Via Gc-fidmentioning
confidence: 99%
“…As a way out, a great deal of research activities nowadays is focused on the application of carbonaceous industrial surplus streams as feedstocks. Just to mention a few, current literature makes us familiar with PHA biosynthesis starting from crude glycerol [7,8], from hydrolyzed polysaccharides [9,10], carbon dioxide from industrial effluent gases [11,12], other C1 compounds [13], waste lipids [14,15], or, as item of interest in the article at hand, lactose from dairy surplus whey [16].…”
Section: Introductionmentioning
confidence: 99%