Novel determination of the total phenolic content in crude plant extracts by the use of 1H NMR of the –OH spectral region

Nerantzaki, Alexandra; Tsiafoulis, Constantinos G.; Charisiadis, Pantelis; Kontogianni, Vassiliki G.; Gerothanassis, Ioannis P.

doi:10.1016/j.aca.2010.12.027

Cited by 49 publications

(42 citation statements)

References 37 publications

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“…A previous method to measure the total phenolic content by 1 H NMR was based on the quantification of resonances of phenolic hydroxyl protons in the δ 8–14 ppm range 18. This method, however, cannot be used to directly measure aqueous solutions such as tea because an aprotic solvent (DMSO- d 6) has to be used.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, we hypothesized that direct NMR spectroscopy might be applicable instead of complex chromatographic separation techniques. So far, NMR was extensively evaluated for the characterization of different types of tea,10–17 however, we were able to find only one article dealing with the application of 1 H-NMR to sage tea and this evaluated only a single parameter (total phenolic content) 18…”

Section: Introductionmentioning

confidence: 99%

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Holistic control of Herbal Teas and Tinctures Based on sage (Salvia officinalis L.) for compounds with Beneficial and Adverse Effects using NMR Spectroscopy

et al. 2012

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A methodology that utilizes 1 H-NMR spectroscopy has been developed to simultaneously analyze toxic terpenes (thujone and camphor), major polyphenolic compounds, the total antioxidant capacity (ORAC) and the Folin-Ciocalteu (FC) index in foods and medicines containing sage. The quantitative determination of rosmarinic acid (limit of detection (LOD) = 10 mg/L) and total thujone (LOD = 0.35 mg/L) was possible using direct integration of the signals. For other parameters (derivatives of rosmarinic acid, carnosol and flavone glycosides, ORAC and FC index), chemometric regression models obtained separately for alcohol-based tinctures (R 2 = 0.94-0.98) and aqueous tea infusions (R 2 = 0.79-0.99) were suitable for screening analysis. The relative standard deviations for authentic samples were below 10%. The developed methodology was applied for the analysis of a wide variety of sage products (n = 108). The total thujone content in aqueous tea infusions was found to be in the range of not detectable (nd) to 37.5 mg/L (average 9.2 mg/L), while tinctures contained higher levels (range nd–-409 mg/L, average 107 mg/L). The camphor content varied from 2.1 to 43.7 mg/L in aqueous infusions and from not detectable to 748 mg/L in tinctures (averages were 14.1 and 206 mg/L, respectively). Phenolic compounds were also detected in the majority of the investigated products. 1 H-NMR spectroscopy was proven to have the ability to holistically control all important adverse and beneficial compounds in sage products in a single experiment, considerably saving time, resources and costs as NMR replaces four separate methodologies that were previously needed to analyze the same parameters.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Holistic control of Herbal Teas and Tinctures Based on sage (Salvia officinalis L.) for compounds with Beneficial and Adverse Effects using NMR Spectroscopy

et al. 2012

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“…The sum of dihydrotanshinone I, cryptotanshinone, tanshinone I and tanshinone II A was calculated as total tanshinones in this research. Total phenolics content was determined using the Folin-Ciocalteu method 102 , with minor modification. Specifically, 0.04 mL extracts, 1 mL distilled water and 1 mL Folin-Ciocaleu reagent were thoroughly mixed.…”

Section: Methodsmentioning

confidence: 99%

SmMYB36, a Novel R2R3-MYB Transcription Factor, Enhances Tanshinone Accumulation and Decreases Phenolic Acid Content in Salvia miltiorrhiza Hairy Roots

Ding

Pei

Bai

et al. 2017

Sci Rep

111

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Phenolic acids and tanshinones are two major bioactive components in Salvia miltiorrhiza Bunge. A novel endogenous R2R3-MYB transcription factor, SmMYB36, was identified in this research. This transcript factor can simultaneously influence the content of two types of components in SmMYB36 overexpression hairy roots. SmMYB36 was mainly localized in the nucleus of onion epidermis and it has transactivation activity. The overexpression of SmMYB36 promoted tanshinone accumulation but inhibited phenolic acid and flavonoid biosynthesis in Salvia miltiorrhiza hairy roots. The altered metabolite content was due to changed metabolic flow which was regulated by transcript expression of metabolic pathway genes. The gene transcription levels of the phenylpropanoid general pathway, tyrosine derived pathway, methylerythritol phosphate pathway and downstream tanshinone biosynthetic pathway changed significantly due to the overexpression of SmMYB36. The wide distribution of MYB binding elements (MBS, MRE, MBSI and MBSII) and electrophoretic mobility shift assay results indicated that SmMYB36 may be an effective tool to regulate metabolic flux shifts.

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“…Aside from as a major analytical tool for structure elucidation of unknown natural products, NMR is also widely used to quantitatively analyze natural products [122] (see Table 2), including (a) small molecular weight chemical compounds such as alcohols [133,134], phenolic acids [125,126], glycosides [124,129], alkaloids [127,130], flavonolignans [131], alkylene [132], heterocyclic compounds [128,141], and terpenoids [123,132] and (b) high molecular weight natural substances such as polysaccharides [142] and lignins [135][136][137][138][139][140]. To obtain accurate quantitative results, it is usually required to optimize qNMR toward given class of compounds.…”

Section: Natural Productsmentioning

confidence: 99%

Quantitative NMR Studies of Multiple Compound Mixtures

2017

Annual Reports on NMR Spectroscopy

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Novel determination of the total phenolic content in crude plant extracts by the use of 1H NMR of the –OH spectral region

Cited by 49 publications

References 37 publications

Holistic control of Herbal Teas and Tinctures Based on sage (Salvia officinalis L.) for compounds with Beneficial and Adverse Effects using NMR Spectroscopy

Holistic control of Herbal Teas and Tinctures Based on sage (Salvia officinalis L.) for compounds with Beneficial and Adverse Effects using NMR Spectroscopy

SmMYB36, a Novel R2R3-MYB Transcription Factor, Enhances Tanshinone Accumulation and Decreases Phenolic Acid Content in Salvia miltiorrhiza Hairy Roots

Quantitative NMR Studies of Multiple Compound Mixtures

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