Novel diagnostics for point-of-care bacterial detection and identification

Reali, Savannah; Najib, Elias; Balázs, Krisztina E. Treuerne; Tan, Adeline Chern Hui; Váradi, Linda; Hibbs, David E.; Groundwater, Paul W.

doi:10.1039/c9ra03118a

Cited by 45 publications

(34 citation statements)

References 68 publications

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“…Aptamers are thermostable and can be reversibly denatured, making them a potent biosensor for PoC tests (Fig 2). These "aptasors" have, for example, been developed for aptamer-linked immunosorbent assays or enzyme-linked oligonucleotide assays also known as enzyme-linked aptamer assays [40][41][42].…”

Section: Biomarker-based Poc Testsmentioning

confidence: 99%

Diagnosing point-of-care diagnostics for neglected tropical diseases

et al. 2021

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Inadequate and nonintegrated diagnostics are the Achilles’ heel of global efforts to monitor, control, and eradicate neglected tropical diseases (NTDs). While treatment is often available, NTDs are endemic among marginalized populations, due to the unavailability or inadequacy of diagnostic tests that cause empirical misdiagnoses. The need of the hour is early diagnosis at the point-of-care (PoC) of NTD patients. Here, we review the status quo of PoC diagnostic tests and practices for all of the 24 NTDs identified in the World Health Organization’s (WHO) 2021–2030 roadmap, based on their different diagnostic requirements. We discuss the capabilities and shortcomings of current diagnostic tests, identify diagnostic needs, and formulate prerequisites of relevant PoC tests. Next to technical requirements, we stress the importance of availability and awareness programs for establishing PoC tests that fit endemic resource-limited settings. Better understanding of NTD diagnostics will pave the path for setting realistic goals for healthcare in areas with minimal resources, thereby alleviating the global healthcare burden.

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Section: Biomarker-based Poc Testsmentioning

confidence: 99%

Diagnosing point-of-care diagnostics for neglected tropical diseases

et al. 2021

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“…This will help in preventing millions of deaths that are recorded annually in many developing countries. [1718]…”

Section: Access Watch Reserve Versus Defined Daily Dose As Metrics mentioning

confidence: 99%

Emerging antimicrobial resistance and newer tools to address the resistance

et al. 2019

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“…Serodiagnostics can be implemented in different formats; however, today two of them dominate in mass practice—microplate enzyme immunoassay and immunochromatographic analysis—which correspond to their leading place in relation to immunodiagnostics in general [ 12 , 13 , 14 ]. At the same time, recent years have seen the accelerated development of point-of-care diagnostic tools, which make it possible to carry out methodically simple testing directly at the sampling site and quickly obtain results [ 15 , 16 , 17 , 18 ]. This requirement is fully met by immunochromatographic analysis (lateral flow immunoassay).…”

Section: Introductionmentioning

confidence: 99%

“…The reaction of immunoglobulin-binding proteins with nonspecific immunoglobulins reduces the proportion of the label that is capable of participating in the formation of a detectable complex of antigen-specific antibody-immunoglobulin-binding protein label when the fluid passes through the analytical zone. simple testing directly at the sampling site and quickly obtain results [15][16][17][18]. This requirement is fully met by immunochromatographic analysis (lateral flow immunoassay).…”

Section: Introductionmentioning

confidence: 99%

Lateral Flow Serodiagnosis in the Double-Antigen Sandwich Format: Theoretical Consideration and Confirmation of Advantages

Sotnikov

Zherdev

Dzantiev

2020

Sensors

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Determination of the presence in the blood of antibodies specific to the causative agent of a particular disease (serodiagnosis) is an effective approach in medical analytical chemistry. Serodiagnostics performed in the lateral flow immunoassay format (immunochromatography) meet the modern requirements for point-of-care testing and are supported by existing technologies of large-scale diagnostic tests production, thus increasing the amount of attention in a tense epidemiological situation. For traditional lateral flow serodiagnostics formats, a large number of nonspecific immunoglobulins in the sample significantly reduces the degree of detectable binding. To overcome these limitations, an assay based on the formation of immobilized antigen-specific antibody-labeled antigen complexes detection was proposed. However, the requirements for its implementation, providing maximum sensitivity, have not been established. This article describes the mathematical model for the above assay. The influence of the ratio of reagent concentrations on the analysis results is considered. It is noted that the formation of specific antibody complexes with several labeled antigens is the main limiting factor in reducing the detection limit, and methods are proposed to minimize this factor. Recommendations for the choice of the assay conditions, following from the analysis of the model, are confirmed experimentally.

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Novel diagnostics for point-of-care bacterial detection and identification

Abstract: The WHO global action plan on antimicrobial resistance outlines the need for new diagnostic tools. Point-of-care testing for bacterial infections would enable clinically meaningful interventions using methods that are rapid, low-cost, easy-to-operate, and portable.

Cited by 45 publications

References 68 publications

Diagnosing point-of-care diagnostics for neglected tropical diseases

Diagnosing point-of-care diagnostics for neglected tropical diseases

Emerging antimicrobial resistance and newer tools to address the resistance

Lateral Flow Serodiagnosis in the Double-Antigen Sandwich Format: Theoretical Consideration and Confirmation of Advantages

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