Novel dipeptidyl peptidase‐IV and angiotensin‐I‐converting enzyme inhibitory peptides released from quinoa protein by in silico proteolysis

Abstract

Quinoa protein has been paid more and more attention because of its nutritional properties and beneficial effects. With the development of bioinformatics, bioactive peptide database and computer‐assisted simulation provide an efficient and time‐saving method for the theoretical estimation of potential bioactivities of protein. Therefore, the potential of quinoa protein sequences for releasing bioactive peptides was evaluated using the BIOPEP database, which revealed that quinoa protein, especially globulin, is a potential source of peptides with dipeptidyl peptidase‐IV (DPP‐IV) and angiotensin‐I‐converting enzyme (ACE) inhibitory activities. Three plant proteases, namely papain, ficin, and stem bromelain, were employed for the in silico proteolysis of quinoa protein. Furthermore, four tripeptides (MAF, NMF, HPF, and MCG) were screened as novel promising bioactive peptides by PeptideRanker. The bioactivities of selected peptides were confirmed using chemical synthesis and in vitro assay. The present work suggests that quinoa protein can serve as a good source of bioactive peptides, and in silico approach can provide theoretical assistance for investigation and production of functional peptides.

Administration with Quinoa Protein Reduces the Blood Pressure in Spontaneously Hypertensive Rats and Modifies the Fecal Microbiota

“…The precipitates were resuspended in water, neutralized, and lyophilized. As reported in our previous study, the yield of quinoa protein extracts and protein purity were 6.51% and 85.23%, respectively [ 24 ].…”

“…Protein ingredients were extracted from Mengli-I quinoa seeds by a previously reported method [ 24 ]. In brief, defatted quinoa flour was suspended in distilled water (1:10, w/w ), protein solubilization was carried out at pH 9.0 by stirring for 60 min, and precipitation was carried out at pH 4.5 followed by centrifugation at 10,000× g for 30 min.…”

“…In the short-term study, SHRs were randomly divided into five groups (5 rats per group). These groups received a saline solution, 10 mg/kg BW captopril, 100 mg/kg, 200 mg/kg, and 400 mg/kg BW quinoa protein, respectively, through oral administration, using syringes [ 24 ]. Each dose of quinoa protein and captopril was dissolved in 1 mL of saline solution.…”

SARS-CoV-2 spike protein-, main protease- and papain-like-protease-targeting peptides from seed proteins following gastrointestinal digestion: An in silico study

“…Nineteen seed storage proteins found in pumpkin, quinoa, rape, sunflower and sesame seeds ( Guo et al., 2020 ; Han et al., 2019 ), were targeted in this study. Quinoa seed protein sequences were retrieved from NCBI ( https://www.ncbi.nlm.nih.gov ) ( Clark et al., 2015 ) on 5 August 2020.…”

“…Seed storage proteins are the major proteins in the five seeds and are likely to be the main sources of bioactive peptides upon oral consumption and GI digestion. The nutritional values and the bioactivity of peptides derived from the five seeds have been reported ( Bao et al., 2020 ; Guo et al., 2020 ; Han et al., 2019 ; Innocent-Ukachi, 2019 ; Vilcacundo et al., 2018 ; Wang et al., 2020b ). Currently, there is still no effective approved treatment or vaccine that can be used to tackle or prevent COVID-19.…”