Novel DNA structures resulting from dTam3 excision in tobacco

Studies on transposable elements of the Ac family have led to different models for excision gap repair in either plants or Drosophila. Excision products generated by the plant transposable elements Ac and Tam3 imply a more or less straightforward ligation of broken ends; excision products of the Drosophila P element indicate the involvement of 'double-strand break' (DSB) repair. Recent findings that excision products of Ac and Tam3 can also contain traces of the element ends indicate, however, that DSB repair might be an alternative repair mechanism in plants. A functional DSB repair mechanism in plants can also be deduced from the observed rapid increases of Ac copy number during plant development and from the involvement of Ac in the generation of internal Ac deletions. On the other hand, alternative repair mechanisms may also be functional in Drosophila, because some of the 'footprints' generated upon P excision can be explained by a mechanism that has been postulated for excision gap repair in plants. It is concluded that plants and Drosophila can use similar repair mechanisms, but that the predominance of a certain repair mechanism is determined by the host.

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Differential repair of excision gaps generated by transposable elements of the ‘Ac family’

Rommens¹,

Haaren²,

Nijkamp³

et al. 1993

BioEssays

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Molecular and functional characterization of Slide, anAc-like autonomous transposable element from tobacco

Grappin¹,

Audéon²,

Chupeau³

et al. 1996

Molec. Gen. Genet.

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A new transposable element of tobacco, Slide, was isolated from the tl mutant line, which shows somatic instability, after its transposition into a locus encoding nitrate reductase (NR). The Slide-124 element is 3733 bp long and its coding sequences show similarities with conserved domains of the transposases of Ac, Tam3 and hobo. Excision from the NR locus is detectable in somatic leaf tissues and Slide mobility is triggered by in vitro tissue culture. Slide excision events create footprints similar to those left by Ac and Tam3. Tobacco lines derived from the tl mutant line seem characterized by unmethylated copies of a few members of the highly repetitive Slide family. Slide mobility was monitored in transient expression assays. In wild-type tobacco protoplasts, the complete Slide element, as well as a defective copy, is able to excise. The complete Slide element, but not the defective version, is able to excise in protoplasts of the heterologous species lettuce (Lactuca sativa). These results show that Slide carries the functions required for its own mobility, and represents the first autonomous Ac-like element characterized in Solanaceae species.

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Evidence forTAM3 activity in transgenicArabidopsis thaliana

Greef

Jacobs

1996

In Vitro Cell.Dev.Biol.-Plant

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Novel DNA structures resulting from dTam3 excision in tobacco

Cited by 5 publications

References 32 publications

Differential repair of excision gaps generated by transposable elements of the ‘Ac family’

Differential repair of excision gaps generated by transposable elements of the ‘Ac family’

Molecular and functional characterization of Slide, anAc-like autonomous transposable element from tobacco

Evidence forTAM3 activity in transgenicArabidopsis thaliana

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