Novel dual-site fluorescent probe for monitoring cysteine and sulfite in living cells

Guo, Xin; Xia, Lili; Huang, Jinxin; Wang, Yiming; Gu, Yueqing; Wang, Peng

doi:10.1039/c8ra01683f

Cited by 12 publications

(3 citation statements)

References 34 publications

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“…Furthermore, BPO-DNSP can not only be used for imaging intracellular SO 2 and biothiols, but can also be used for monitoring the conversion from biothiols to SO 2 without adding exogenous enzymes, which can help us better understand the physiological nature of RSS. In comparison with the reported probes [24,25,26,27,28] for two-channel imaging of SO 2 and biothiols, BPO-DNSP exhibited three advantages for bioimaging: (1) as far as 170 nm emission shift from two different channels; (2) near-infrared wavelength detection of intracellular biothiols; and (3) monitoring the conversion from biothiols to SO 2 without adding exogenous enzymes. Moreover, BPO-DNSP revealed its superiority in biological applications as it avoids potential interference compared with reported probes using an aldehyde group as a reaction site.…”

Section: Discussionmentioning

confidence: 98%

“…To better understand their physiological nature and cellular cross-talk, a single fluorescent probe with dual functions could provide more detailed information about the microenvironment and physical condition of the cells [24]. However, there are still few fluorescent probes presented for imaging SO 2 and biothiols with distinct responses from different emission channels [25,26,27,28].…”

Section: Introductionmentioning

confidence: 99%

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Lysosome-Targeted Single Fluorescence Probe for Two-Channel Imaging Intracellular SO2 and Biothiols

et al. 2019

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As the members of reactive sulfur species, SO2 and biothiols play a significant role in physiological and pathological processes and directly influence numerous diseases. Furthermore, SO2 and biothiols can provide a reductive environment for lysosomes to carry out their optimal functionality. To this end, the development of single fluorescent probes for imaging SO2 and biothiols from different emission channels is highly desirable for understanding their physiological nature. Here, a lysosome-targeted fluorescent probe (BPO-DNSP) with a dual reaction site for SO2 and biothiols was presented. BPO-DNSP can sensitively and selectively respond to SO2 in the green channel with a large Stokes shift over 105 nm, and to biothiols in the near-infrared emission channel with a large Stokes shift over 109 nm. The emission shift for the two channels was as high as 170 nm. Colocalization experiments verified that BPO-DNSP can selectively enrich lysosomes. Notably, BPO-DNSP can not only be used to image intracellular SO2 and biothiols from two different channels, but also to monitor the conversion of biothiols to SO2 without adding exogenous enzymes in living HeLa cells.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Lysosome-Targeted Single Fluorescence Probe for Two-Channel Imaging Intracellular SO2 and Biothiols

et al. 2019

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“…The other select anions and structurally similar sulfur-containing oxyanions (1000 μM; Figure 4c) does not exerted any noticeable variation to the SO 3 2À -actuated spike in the native fluorescence profile of D. Concomitantly, a probable alteration in the fluorescence of D by nucleophilic biothiols (Cys, Hcys and GSH; 1000 μM) was also negligible, thereby ruling out a possible interference from the latter in the effective sensing of sulfite ions by D (Figure 4d). [38,39] Therefore, it could be concluded that the molecule D offers a discriminating behavior towards SO 3 2À ions from its chemically close sulfur associates via selectively sensitive fluorescence stimulation.…”

Section: Chemistryselectmentioning

confidence: 96%

Highly Sensitive and Selective Sulfite Ion Sensor Based on Biochemically Stable Iodo‐Functionalized Coumarin Fluorophores

et al. 2022

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Belonging to the class of sulfur oxyanions, the physiologically prevalent sulfite (SO32−) species has been serving as a popular synthetic additive to enhance the shelf life of a plethora of foods and beverages like bread and wine. On the contrary, an impermissible endogenous accumulation of this reactive sulfur species via multiple factors has been concerned in the functioning of skin disorders and neurodegenerative diseases like Alzheimer's. Accordingly, a pivotal role of sulfite in food quality and human health warrants a reliable analytical technique for the rapid and sensitive detection of SO32− ions in environmental specimens. This article features a new‐fangled iodo‐functionalized coumarin molecule (D), followed by an exploration of its analytical utility as a selectively sensitive sensor for SO32− ions via “switch‐on” fluorescence. In stark contrast to its poorly ion‐discriminating iodine‐free analogue (E) as reported in the literature, the new fluorophore D was capable of rapidly sniffing out sulfite ions (∼3 min) in a specific range of 1000–1 μM, in the back‐drop of a diverse array of environmentally relevant anions at an ultra‐low concentration of 77 nM in semi‐aqueous medium by using simple and cheap fluorescence spectroscopy. The formed sensor found highly selective and sensitive in presence of wide variety of interfering ions and biothiols, which suggest their extreme utilization in the real samples. According to best of our knowledge, it is the first report, which can detection sulfite ion at very low concentration level and it work efficiently under UV‐illumination with bright fluorescence. A comparable sensitivity of D towards the target analyte (SO32− ions) was also recorded in real samples i. e. fresh real water samples and fresh juice specimens.

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Near-infrared fluorogenic receptor for selective detection of cysteine in blood serum and living cells

2021

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Novel dual-site fluorescent probe for monitoring cysteine and sulfite in living cells

Abstract: A dual-site fluorescence probe LC-1 was developed for the detection of Cys and SO32−.

Cited by 12 publications

References 34 publications

Lysosome-Targeted Single Fluorescence Probe for Two-Channel Imaging Intracellular SO2 and Biothiols

Lysosome-Targeted Single Fluorescence Probe for Two-Channel Imaging Intracellular SO2 and Biothiols

Highly Sensitive and Selective Sulfite Ion Sensor Based on Biochemically Stable Iodo‐Functionalized Coumarin Fluorophores

Near-infrared fluorogenic receptor for selective detection of cysteine in blood serum and living cells

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