Novel durum wheat genes up-regulated in response to a combination of heat and drought stress

Rampino, Patrizia; Mita, Giovanni; Fasano, Pasqua; Borrelli, Grazia Maria; Aprile, Alessio; Dalessandro, Giuseppe; Bellis, Luigi De; Perrotta, Carla

doi:10.1016/j.plaphy.2012.04.006

Cited by 80 publications

(62 citation statements)

References 35 publications

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“…Up-regulation by 2-fold and down-regulation by 0.5-fold were considered as signifi cant. *signifi cant diff erence at p<0.05; **highly signifi cant diff erence at p<0.01; without asterisk=no signifi cant diff erence observed the la er observation and is in agreement with other transcriptomics analyses (34)(35)(36).…”

Section: Discussionsupporting

confidence: 90%

“…They might correspond to genes not yet characterized in any plant species. Several studies have reported the identifi cation of new transcripts using the same approach in other plants (34,(76)(77)(78). The characterization of genes not yet described in databases should be considered because it may provide new information about the plant stress response.…”

Section: Discussionmentioning

confidence: 99%

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Identification of Differentially Expressed Genes by cDNA-AFLP Technique in Response to Drought Stress in Triticum durum

Melloul

Iraqi²,

Alaoui

et al. 2014

Food. Technol. Biotechnol.

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SummaryDrought is the single largest abiotic stress factor leading to reduced crop yields. The identifi cation of diff erentially expressed genes and the understanding of their functions in environmentally stressful conditions are essential to improve drought tolerance. Transcriptomics is a powerful approach for the global analysis of molecular mechanisms under abiotic stress. To identify genes that are important for drought tolerance, we analyzed mRNA populations from untreated and drought-stressed leaves of Triticum durum by cDNA --amplifi ed fragment length polymorphism (cDNA-AFLP) technique. Overall, 76 transcript--derived fragments corresponding to diff erentially induced transcripts were successfully sequenced. Most of the transcripts identifi ed here, using basic local alignment search tool (BLAST) database, were genes belonging to diff erent functional categories related to metabolism, energy, cellular biosynthesis, cell defense, signal transduction, transcription regulation, protein degradation and transport. The expression pa erns of these genes were confi rmed by quantitative reverse transcriptase real-time polymerase chain reaction (qRT--PCR) based on ten selected genes representing diff erent pa erns. These results could facilitate the understanding of cellular mechanisms involving groups of genes that act in coordination in response to stimuli of water defi cit. The identifi cation of novel stress-responsive genes will provide useful data that could help develop breeding strategies aimed at improving durum wheat tolerance to fi eld stress.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Identification of Differentially Expressed Genes by cDNA-AFLP Technique in Response to Drought Stress in Triticum durum

Melloul

Iraqi²,

Alaoui

et al. 2014

Food. Technol. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Furthermore, some 7 reports indicate that it is not possible to extrapolate plant responses to combined stresses 8 starting from the response derived from a single stress (Rampino et al, 2012).…”

mentioning

confidence: 99%

Carbon balance, partitioning and photosynthetic acclimation in fruit-bearing grapevine (Vitis vinifera L. cv. Tempranillo) grown under simulated climate change (elevated CO2, elevated temperature and moderate drought) scenarios in temperature gradient greenhouses

Salazar-Parra

Aranjuelo

Pascual

et al. 2015

Journal of Plant Physiology

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“…However, heat stress is often linked with reduced water availability. Water defi cit is significantly higher under the combined heat and drought stress rather than each single condition (Rampino et al 2012 ).…”

Section: Water Relationsmentioning

confidence: 88%

“…In order to develop multiple stresstolerant crops through genetic manipulation, it is necessary to understand the molecular mechanism underlying the response of crop plants to the combination of abiotic stresses. Studies on tobacco, Arabidopsis, and wheat suggest that the response to the combination of stresses is very different from each individual stress (Rampino et al 2012 ;Rizhsky et al 2002Rizhsky et al , 2004. For example, a comparison of the effects between heat, drought, and combined stress on wheat plants showed a higher number of up-regulated genes in combined stressed plants with respect to each individual.…”

Section: The Molecular and Genomic Responses To Heat Stressmentioning

confidence: 99%

Molecular Physiology of Heat Stress Responses in Plants

Hemmati

Gupta

Basu

2015

Elucidation of Abiotic Stress Signaling in Plants

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Heat stress is one of the major abiotic stresses that plants encounter. Heat stress causes billions of dollars in losses of agricultural crops worldwide. Here, we summarize the molecular and whole genome responses due to heat stress in plants. It has been reported that there are cascades of biochemical reactions that lead to heat stress. In most cases, heat stress is coupled with drought stress response. With the advancements in genomic tools, we have more information on genes, which are upor down-regulated in plants due to heat stress. The heat-stressed plants may exhibit various physiological responses, including stomatal closure, suppressed photosynthesis, stunted growth, etc. Microarray and transcriptome sequencing gave us the tools to perform genome-wide expression profi ling in heat-stressed plants. Understanding how gene expression in heat-stressed plants works will help us to discover novel heat stress-tolerant genes. These genes could be overexpressed in crop plants to make transgenic heat-tolerant agricultural crops. Climate change and global warming are major concerns for us and production of thermotolerant plants could address the issue of global crop loss due to heat and drought stresses.

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Novel durum wheat genes up-regulated in response to a combination of heat and drought stress

Cited by 80 publications

References 35 publications

Identification of Differentially Expressed Genes by cDNA-AFLP Technique in Response to Drought Stress in Triticum durum

Identification of Differentially Expressed Genes by cDNA-AFLP Technique in Response to Drought Stress in Triticum durum

Carbon balance, partitioning and photosynthetic acclimation in fruit-bearing grapevine (Vitis vinifera L. cv. Tempranillo) grown under simulated climate change (elevated CO2, elevated temperature and moderate drought) scenarios in temperature gradient greenhouses

Molecular Physiology of Heat Stress Responses in Plants

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