Novel electrochemical sensor system for protein using the aptamers in sandwich manner

Ikebukuro, Kazunori; Kiyohara, Chiharu; Sode, Koji

doi:10.1016/j.bios.2004.09.002

Cited by 264 publications

(169 citation statements)

References 17 publications

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“…Since glucosesensing systems are well-established and used commercially, they are attractive tools for sensing systems of various biomarkers that use aptamers. We first reported thrombin sensing using an aptamer conjugated with pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQGDH) Ikebukuro et al, 2005a). PQQGDH has a high catalytic activity (about 5000 U/mg protein).…”

Section: Oxidoreductasementioning

confidence: 99%

Aptamer Sensors Combined with Enzymes for Highly Sensitive Detection

Abe¹

2011

Biosensors - Emerging Materials and Applications

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Section: Oxidoreductasementioning

confidence: 99%

Aptamer Sensors Combined with Enzymes for Highly Sensitive Detection

Abe¹

2011

Biosensors - Emerging Materials and Applications

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“…The first G4 electrochemical aptasensor reported presented an aptamer-analyte-aptamer sandwich-type format, being developed for thrombin detection [64,65]. The sensor was built up by two aptamers, the first aptamer immobilized onto the gold electrode for capturing the thrombin onto it and the second one, a glucose dehydrogenase (GDH)-labelled anti-thrombin aptamer.…”

Section: Sandwich-type G4 Electrochemical Aptasensormentioning

confidence: 99%

“…The first aptamer was generally immobilized onto gold via a thiol [56][57][58][59][60] and, more recently, magnetic beads [61,62]. The labels of the second aptamer were either redox molecules, nanocomposites [60], nanoparticles [57,63], quantum dots [58,59] or enzymes, with catalytic activity that transformed the substrate into an electroactive product [56,64,65].…”

Section: Sandwich-type G4 Electrochemical Aptasensormentioning

confidence: 99%

Guanine Quadruplex Electrochemical Aptasensors

Chiorcea-Paquim

Oliveira-Brett

2016

Chemosensors

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Guanine-rich nucleic acids are able to self-assemble into G-quadruplex four-stranded secondary structures, which are found at the level of telomeric regions of chromosomes, oncogene promoter sequences and other biologically-relevant regions of the genome. Due to their extraordinary stiffness and biological role, G-quadruples become relevant in areas ranging from structural biology to medicinal chemistry, supra-molecular chemistry, nanotechnology and biosensor technology. In addition to classical methodologies, such as circular dichroism, nuclear magnetic resonance or crystallography, electrochemical methods have been successfully used for the rapid detection of the conformational changes from single-strand to G-quadruplex. This review presents recent advances on the G-quadruplex electrochemical characterization and on the design and applications of G-quadruplex electrochemical biosensors, with special emphasis on the G-quadruplex aptasensors and hemin/G-quadruplex peroxidase-mimicking DNAzyme biosensors.

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“…DNA aptamers sensitive to fibrinogen and heparin binding sites of thrombin have been used successfully in the development of biosensors and in signal amplification. In the sandwich assay reported by Ikebukuro et al (2005), thiolated capture 15-mer TBA aptamers were immobilised on a gold surface through chemisorption. Another reported 29-mer aptamer sensitive to heparin binding site at thrombin was linked to pyrroquinoleine quinine glucose dehydrogenase (PQQGDH).…”

Section: Dna Aptamers Containing G-quadruplexes In Biosensorsmentioning

confidence: 99%

Potential uses of G-quadruplex-forming aptamers

Víglaský

Hianik

2013

gpb

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Abstract. Guanine quadruplex (G-quadruplex) structures are one of a number of structures which are capable of adopting aptamers. G-rich DNA or RNA has an increased propensity to form quadruplex structures which have unusual biophysical and biological properties. G-rich aptamers which form G-quadruplexes have several advantages over unstructured sequences: G-quadruplexes are non-immunogenic, thermodynamically and chemically stable and they have both higher resistance to various serum nucleases and an enhanced cellular uptake. These advantages have led to a number of synthetic oligonucleotides being studied for their potential use as therapeutic agents for cancer therapy and in the treatment of various other diseases. In addition to their suitability in the fields of medicine and biotechnology, these, highly specified, aptameric G-quadruplexes also have great potential in the further development of nano-devices; e.g. basic components in microarrays, microfluidics, sandwich assays and electrochemical biosensors. This review summarizes the biophysical properties of G-quadruplexes and highlights the importance of the stability and recognition properties of aptamers. Examples of the application of aptamers in medical therapy and in biosensors are also discussed.

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Novel electrochemical sensor system for protein using the aptamers in sandwich manner

Cited by 264 publications

References 17 publications

Aptamer Sensors Combined with Enzymes for Highly Sensitive Detection

Aptamer Sensors Combined with Enzymes for Highly Sensitive Detection

Guanine Quadruplex Electrochemical Aptasensors

Potential uses of G-quadruplex-forming aptamers

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