Novel ESTs from a Jute (Corchorus olitorius L.) cDNA Library

Abstract

The paper describes the identification of new expressed sequence tags (ESTs) from a cDNA library of Corchorus olitorius L. var. O-4 after the initial description of the library construction reported recently. The sequence homology search in The Arabidopsis Information Source (TAIR) using the WU-BLAST tool revealed four complete and ten partial cDNA sequences. The complete cDNA sequences, based on their similarities to those of Arabidopsis proteins, encode V-ATPase subunit F, mitochondrial NADH-ubiquinone oxidoreductase, RuBisCO small subunit 1A and heat shock protein (HSP 60). Based on similar homology results the partial cDNAs encode proteins, namely, chaperonin, actin 7, RelA-spoT homology, 60s ribosomal protein L 36a, transport protein, chloroplast inner membrane import protein Tic22, formate dehydrogenase, serine hydroxymethyltransferase, metallothionein 2B and tansmembrane kinase. These cDNA sequences are new additions to the ESTs that encode chitinase-like protein (Class I) and 60S acidic ribosomal protein reported earlier. All the available cDNA sequences including that for the chitinase-like protein have been registered with GenBank, bearing Accession Nos. EU024510 through EU024520 and EU092254, EU057193-95. The cDNA samples are available for any researchers interested in such ESTs. A Clustal-W study of the amino acid sequences of proteins encoded by cDNA isolated in the present investigation with those of cotton, citrus, Arabidopsis, tobacco and other organisms revealed that the homology is maximum between jute and cotton followed by citrus, grapevine, tobacco and Arabidopsis.

Construction of a full-length cDNA library and analysis of expressed sequence tags in white jute (Corchorus capsularis L.)

“…Moreover, our study indicated unigenes of C. capsularis have highest homology to P. trichocarpa, and which also have higher homology to R. communis, C. olitorius and V. vinifera. The results are different with Wazni et al, who reported the homology was maximum between black jute (olitorius) and cotton followed by citrus, grapevine, tobacco and arabidopsis (Wazni et al, 2007), which illustrates the differences between black and white jute. Above results would be a potential resource for comprehensive genomic studies in Corchurus species.…”

“…Jute fibres exhibit a characteristically high luster, good moisture absorption performance, rapid water loss capacity and easy degradation (Zhang et al, 2013). The textile and paper industry are interested in its potential as an important ingredient for producing paper, fine textiles as well as a renewable source for biofuel (Wazni et al, 2007). In recent years, with increasing uses of jute in diversified industries, there is a growing demand for highyielding new cultivars.…”

Determining Genetic Diversity of Deshi Jute (Corchorus capsularis) for the Improvement of Fibre Yield and Associated Traits

“…Jute is important for the textile and paper industries because it is a valuable ingredient for the production of paper and Determining genetic diversity of deshi jute (Corchorus capsularis) for the improvement of fibre yield and associated traits quality textiles as well as a renewable source of biofuels [10]. Jute fibers characteristically show high gloss, good moisture absorption performance, rapid dehydration capacity and easy degradation [11].…”

Modification of Monolignol Biosynthetic Pathway in Jute: Different Gene, Different Consequence

“…Biodegradable nature of jute fiber and the potential high yield of cellulose biomass per acre have increased the global interest in jute. Textile and paper industries are also interested in its potential as an important ingredient for producing paper and fine textiles14. As with other lignocellulosic plants the drawback of jute when used as a source for such purposes is the abundance of lignin polymer that renders the plant material almost inaccessible for downstream processes.…”