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The paper describes the identification of new expressed sequence tags (ESTs) from a cDNA library of Corchorus olitorius L. var. O-4 after the initial description of the library construction reported recently. The sequence homology search in The Arabidopsis Information Source (TAIR) using the WU-BLAST tool revealed four complete and ten partial cDNA sequences. The complete cDNA sequences, based on their similarities to those of Arabidopsis proteins, encode V-ATPase subunit F, mitochondrial NADH-ubiquinone oxidoreductase, RuBisCO small subunit 1A and heat shock protein (HSP 60). Based on similar homology results the partial cDNAs encode proteins, namely, chaperonin, actin 7, RelA-spoT homology, 60s ribosomal protein L 36a, transport protein, chloroplast inner membrane import protein Tic22, formate dehydrogenase, serine hydroxymethyltransferase, metallothionein 2B and tansmembrane kinase. These cDNA sequences are new additions to the ESTs that encode chitinase-like protein (Class I) and 60S acidic ribosomal protein reported earlier. All the available cDNA sequences including that for the chitinase-like protein have been registered with GenBank, bearing Accession Nos. EU024510 through EU024520 and EU092254, EU057193-95. The cDNA samples are available for any researchers interested in such ESTs. A Clustal-W study of the amino acid sequences of proteins encoded by cDNA isolated in the present investigation with those of cotton, citrus, Arabidopsis, tobacco and other organisms revealed that the homology is maximum between jute and cotton followed by citrus, grapevine, tobacco and Arabidopsis.

Purpose: To quantify the relationship between the post‐treatment [18F]‐2‐fluoro‐2‐deoxyglucose (18F‐FDG) positron emission tomography (PET) uptake and radiation dose received and correlate this relationship with prospectively scored pulmonary radiation toxicity. Methods: 34 patients treated for esophageal cancer with thoracic radiotherapy were evaluated prospectively at the MD Anderson Cancer Center. The treatment planning computed tomography (CT) imaging was registered with the restaging PET/CT. Using histogram analysis, the voxel average FDG‐PET uptake vs. radiation dose was obtained for each case and linear regression was performed. The slope of the linear regression was termed the pulmonary metabolic response rate (PMRR). Common Toxicity Criteria version 4 was used to score pneumonitis. Exhaled nitric oxide (eNO) was measured on the first and last days of radiation treatment. Receiver operating characteristic curves were used to determine the threshold PMRR, mean lung dose, volume of lung receiving 5, 10, 20 and 30 Gy and eNO ratio (End/beginning) that can best predict symptomatic patients. Results: 29 patients were evaluable. The median of the mean standard uptake value from lung that received 0–5 Gy was 0.51 (range, 0.34–1.24), 5–10 Gy was 0.71 (range, 0.39–1.32), 10–20 Gy was 0.78 (0.40–1.56), and > 20 Gy was 1.10 (range, 0.43–3.01). 25 patients had grade 0 or 1 pulmonary symptom score and 4 patients had grade 2 or higher (symptomatic) score. Both PMRR and the eNO ratio gave a significant area under the curve of 0.86 (p = 0.02) and 1 (p<0.001) respectively. Using a PMRR threshold of 0.0184 yielded a true positive rate of 0.75 and false positive rate of 0.125. Conclusions: In this prospective study, the PMRR predicted for development of radiation pneumonitis. This unique quantitative assessment of radiation pnuemonitis can be used for quality control to deliver higher doses of radiation safely.

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