Novel G10P[14] Rotavirus Strain, Northern Territory, Australia

Cowley, Daniel; Donato, Celeste; Roczo-Farkas, Susie; Kirkwood, Carl D.

doi:10.3201/eid.1908.121653

Cited by 20 publications

(16 citation statements)

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“…RNA was manually extracted from 140 µL of NP swabs and saliva, 280 µL of urine and plasma and 140 µL of 20% (w/v) faecal suspension 19 and then eluted in 50 to 60 µL sterile, molecular water (Life technologies, Australia), using the QIAamp viral RNA kit (QIAgen GmbH, Hilden, Germany) according to the manufacturer's instructions. A previously published RT-PCR protocol targeting the RdRp gene was used on an ABI 7500 20 .…”

Section: Sars-cov-2 Detectionmentioning

confidence: 99%

Immune responses to SARS-CoV-2 in children of parents with symptomatic COVID-19

Tosif

Neeland

Sutton

et al. 2020

Preprint

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Compared to adults, children with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have mild or asymptomatic infection, but the underlying immunological differences remain unclear. We describe clinical features, virology, longitudinal cellular and cytokine immune profile, SARS-CoV-2-specific serology and salivary antibody responses in a family of two parents with PCR-confirmed symptomatic SARS-CoV-2 infection and their three children, who were repeatedly SARS-CoV-2 PCR negative. Cellular immune profiles and cytokine responses of all children were similar to their parents at all timepoints. All family members had salivary anti-SARS-CoV-2 antibodies detected, predominantly IgA, that coincided with symptom resolution in 3 of 4 symptomatic members. Plasma from both parents and one child had IgG antibody detected against the S1 protein and virus neutralising activity ranging from just detectable to robust titers. Using a systems serology approach, we show that all family members demonstrated higher levels of SARS-CoV-2-specific antibody features than healthy controls. These data indicate that children can mount an immune response to SARS-CoV-2 without virological evidence of infection. This raises the possibility that despite chronic exposure, immunity in children prevents establishment of SARS-CoV-2 infection. Relying on routine virological and serological testing may therefore not identify exposed children, with implications for epidemiological and clinical studies across the life-span.

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Section: Sars-cov-2 Detectionmentioning

confidence: 99%

Immune responses to SARS-CoV-2 in children of parents with symptomatic COVID-19

Tosif

Neeland

Sutton

et al. 2020

Preprint

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“…Recently, more P[14] strains have been associated with gastroenteritis in humans and most of the reported P[14] strains have the Genogroup 2 gene constellation (Banyai et al, 2009; Banyai et al, 2010; Cowley et al, 2013; Donato et al, 2014; El Sherif et al, 2011; Ghosh et al, 2007; Matthijnssens et al, 2009; Medici et al, 2008; Mullick et al, 2013). Human P[14] strains have common origins with those of the even-toed ungulates belonging to the mammalian order Artiodactyla (Matthijnssens et al, 2009).…”

mentioning

confidence: 99%

Full genomic characterization and phylogenetic analysis of a zoonotic human G8P[14] rotavirus strain detected in a sample from Guatemala

Gautam

Mijatovic-Rustempasic

Roy

et al. 2015

Infection, Genetics and Evolution

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We report the genomic characterization of a rare human G8P[14] rotavirus strain, identified in a stool sample from Guatemala (GTM) during routine rotavirus surveillance. This strain was designated as RV A/Human-wt/GTM/2009726790/2009/G8P[14], with a genomic constellation of G8-P[14]-I2-R2-C2-M2-A13-N2-T6-E2-H3. The VP4 gene occupied lineage VII within the P[14] genotype. Phylogenetic analysis of each genome segment revealed close relatedness to several zoonotic simian, guanaco and bovine strains. Our findings suggest that strain RVA/Human-wt/GTM/2009726790/2009/G8P[14] is an example of a direct zoonotic transmission event. The results of this study reinforce the potential role of interspecies transmission and reassortment in generating novel and rare rotavirus strains which infect humans.

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“…These P[14] strains exhibit notable genetic variation with an overall mean diversity of 86.4% (range 79.3–100% identity). The VP4 gene of strain RVA/Human-wt/BRB/CDC1133/2012/G4P[14] shared the highest degree of nucleotide sequence identity with P[14] strains from an human outbreak in Northern Territory, Australia, in 2011 (Cowley et al, 2013) with 91.2% identity and 88.2% or less with other P[14] strains. Within P[14] strain genes, we identified 7 lineages with strong bootstrap support and a minimum of 10% genetic distance between lineages (Figure 1B).…”

mentioning

confidence: 99%

“…Lineages I, III, IV and VII contain G6, G8 and G10 strains isolated from bovines, guanacos, sheep, and other ungulates. Strain RVA/Human-wt/BRB/CDC1133/2012/G4P[14] VP4 gene occupies lineage VI shared with the aforementioned P[14] strains from Australia (Figure 1B) (Cowley et al, 2013). Unlike the Barbados strain, the Australian P[14] strains possess a G10-P[14]-I2-R2-C2-M2-A11-N2-T6-E2-H3 Genogroup 2 (DS-1 like) genome constellation which is consistent with G6P[14] and G8P[14] strains identified globally (Matthijnssens et al, 2009b).…”

mentioning

confidence: 99%

“…Unlike the Barbados strain, the Australian P[14] strains possess a G10-P[14]-I2-R2-C2-M2-A11-N2-T6-E2-H3 Genogroup 2 (DS-1 like) genome constellation which is consistent with G6P[14] and G8P[14] strains identified globally (Matthijnssens et al, 2009b). In recent years, more P[14] strains have been associated with gastroenteritis in humans and most of the reported P[14] strains have the Genogroup 2 gene constellation, with one or more gene segments that are of bovine origin (Banyai et al, 2009; Banyai et al, 2010; Cowley et al, 2013; Donato et al, 2014; El Sherif et al, 2011; Ghosh et al, 2007; Matthijnssens et al, 2009b; Medici et al, 2008; Mullick et al, 2012). The VP8* region of the P[14] VP4 protein has been shown to interact with the type A histo blood group antigens of humans (Hu et al, 2012; Liu et al, 2012) as well as bovine and porcine mucins (Liu et al, 2012) and this is thought to play a role in cross-species transmission of P[14] RVAs.…”

mentioning

confidence: 99%

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Full genomic characterization of a novel genotype combination, G4P[14], of a human rotavirus strain from Barbados

Tam

Roy

Esona

et al. 2014

Infection, Genetics and Evolution

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Since 2004, the Pan American Health Organization (PAHO) has carried out rotavirus surveillance in Latin America and the Caribbean. Here we report the characterization of human rotavirus with the novel G-P combination of G4P[14], detected through PAHO surveillance in Barbados. Full genome sequencing of strain RVA/Human-wt/BRB/CDC1133/2012/G4P[14] revealed that its genotype is G4-P[14]-I1-R1-C1-M1-A8-N1-T1-E1-H1. The possession of a Genogroup 1 (Wa-like) backbone distinguishes this strain from other P[14] rotavirus strains. Phylogenetic analyses suggested that this strain was likely generated by genetic reassortment between human, porcine and possibly other animal rotavirus strains and identified 7 lineages within the P[14] genotype. The results of this study reinforce the potential role of interspecies transmission in generating human rotavirus diversity through reassortment. Continued surveillance is important to determine if rotavirus vaccines will protect against strains that express the P[14] rotavirus genotype.

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Novel G10P[14] Rotavirus Strain, Northern Territory, Australia

Cited by 20 publications

References 20 publications

Immune responses to SARS-CoV-2 in children of parents with symptomatic COVID-19

Immune responses to SARS-CoV-2 in children of parents with symptomatic COVID-19

Full genomic characterization and phylogenetic analysis of a zoonotic human G8P[14] rotavirus strain detected in a sample from Guatemala

Full genomic characterization of a novel genotype combination, G4P[14], of a human rotavirus strain from Barbados

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