Novel gel-based rapid test for non-instrumental detection of ochratoxin A in beer

Abstract

A rapid easy-to-use immunoassay was optimised for the non-instrumental detection of ochratoxin A (OTA) in beer. The analytical method involves preconcentration on the immunoaffinity layer inside a column followed by direct competitive ELISA detection in the same layer. The visual cut-off value, i.e. the lowest OTA concentration resulting in no colour development, was 0.2 microg L(-1). Assay validation was performed using samples spiked with OTA. Thirty-seven naturally contaminated samples were screened with the gel-based method developed and no false-negative results were obtained. The method described offers a simple, rapid and cost-effective screening tool, thus contributing to better health protection of consumers.

Immunoaffinity pre-concentration combined with on-column visual detection as a tool for rapid aflatoxin M1 screening in milk

“…In our previous research a gel-based immunoassay in combination with a clean-up layer (clean-up tandem immunoassay column) was developed for the detection of ochratoxin A in highly coloured food samples: coffee (Lobeau, De Saeger, Sibanda, Barna-Vetro, & Van Peteghem, 2005), cocoa (Lobeau, De Saeger, Sibanda, Barna-Vetro, & Van Peteghem, 2007), spices (Goryacheva et al, 2006(Goryacheva et al, , 2007 and beer (Goryacheva et al, 2008). The clean-up layer reduced the colour of the extract and suppressed matrix interferences on the detection immunolayer.…”

Immunochemical methods for rapid mycotoxin detection in food and feed

“…So, the successive distribution of the components between different layers was realized, similar to immunochromatographic techniques. This approach was applied to OTA detection in beer (Goryacheva et al, 2008a) and simultaneous detection of ZEA and T-2 in feed samples (Basova et al, 2010).…”

“…It allowed use of high sample volumes to improve assay sensitivity. In particular, to obtain a cut-off level of 0.2 µg l -1 for OTA in beer, a beer sample volume of 12 ml was used (Goryacheva et al, 2008a), whereas 10 ml of centrifuged skimmed milk was used to reach a cut-off level of 0.04 µg l -1 in the determination of AfM 1 in milk and milk products (Goryacheva et al, 2009a). Table 5.4 summarizes data on developed gel-based tests for the determination of mycotoxins.…”

Sensitive detection of ochratoxin A in wine and cereals using fluorescence-based immunosensing

“…These tools should protect consumers from the intake of OTA-contaminated food (Pittet, 2005;Prieto-Simón, Noguer, & Campàs, 2007). Dipsticks, based on immunoassays and a direct visual reading, have been developed as simple and powerful tools for the preliminary screening of OTAcontaminated samples (Bazin, Nabais, & Lopez-Ferber, 2010;Goryacheva et al, 2008;Kolosova, Saeger, Eremin, & Van Peteghem, 2007;Schneider et al, 2004;Shim, Dzantiev, Eremin, & Chung, 2009a;Shim, Kim, Ryu, Nam, & Chung, 2009b). They are cost-effective and allow fast analysis.…”