Novel greenly synthesized titanium dioxide nanoparticles compared to liposomes in drug delivery: in vivo investigation on Ehrlich solid tumor model

Abstract

Aims In a previous work, a pure crystalline titanium dioxide nanoparticles (TiO 2 NPs) were synthesized by green synthesis technique using Aloe vera leaves extract as reducing agent. In this work, we are aiming to investigate the potential of the novel greenly synthesized TiO 2 NPs as a nano-drug delivery system for the anticancer drug, doxorubicin (Dox). Main methods The cytotoxicity of the synthesized TiO 2 NPs was tested on two cell lines; normal human skin fibroblasts (HSF) and breast adenocarcinoma cells (MCF-7). Then, Dox was loaded to both TiO 2 NPs (Dox- TiO 2 NPs) and liposomes (Dox-Lip). The loaded nanoparticles were characterized by TEM, FTIR, encapsulation efficiency, particle size and zeta potential measurement. Moreover, in vitro drug release was studied. Ehrlich tumor-bearing mice were used to study the anticancer activity of Dox- TiO 2 NPs, Dox-Lip, and aqueous Dox solution. Tumor volume, survival rate, and histopathological alterations were compared in all groups. Key findings Dox was successfully loaded to both liposomes and TiO 2 NPs with an encapsulation efficiency of 77% and 65%, respectively. The particle size of Dox-TiO 2 NPs, and Dox-Lip was 14.53 nm, and 103 nm, respectively. The cumulative Dox released from TiO 2 NPs and liposomes after 4 h was 18 and 46%, respectively. Dox-Lip and Dox-TiO 2 NPs resulted in the highest degree of tumor growth inhibition with 100% and 83% of treated animals remained alive, respectively. Significance The greenly synthesized TiO 2 NPs were proved to be as effective as liposomes in enhancing the anticancer activity of Dox.

Rapid green-synthesis of TiO2 nanoparticles for therapeutic applications

“…The encapsulation of T1, T2 and T3 was realised by following the procedure mentioned elsewhere 19 with minor modifications. Three sets of drug-NPs solutions were prepared.…”

“… 18 The smaller the NPs, the more penetrating power they possess. Fadeel et al 19 synthesised anatase TiO 2 NPs having sizes in the range 13–18 nm using aloe vera leaf extract. They were loaded with doxorubicin (DOX) exhibiting high encapsulation efficiency.…”

Nanoengineered chitosan functionalized titanium dioxide biohybrids for bacterial infections and cancer therapy

“…1 a. The pattern observed in the lower region of 2θ from 10 to 27° indicates the characteristic peak of chitosan 50 , 51 . The XRD peaks observed at 27.214, 35.855, 38.987, 41.019, 43.845, 54.1, 56.425, 62.495, 63.824, 68.771, 69.558, 72.267 and 76.454° correspond to (110), (101), (200), (111), (210), (211), (220), (002), (310), (301), (112), (311) and (202) planes respectively.…”

Physical characterization, biocompatibility, and antimicrobial activity of polyvinyl alcohol/sodium alginate blend doped with TiO2 nanoparticles for wound dressing applications

“…The bands observed at 3260 ± 4 cm −1 in all the studied samples were assigned to the stretching vibration of -OH groups of SA 46 . The IR band at 1325.5 ± 1.5 cm −1 was ascribed to the stretching vibration of Ti–O–Ti of TiO 2 NPs 47 and also to C–O asymmetrical stretching of SA 48 . Changes in the fingerprint regions of the IR spectrum, as well as in the shape and intensity of bands at a range of 1597–1645 cm −1 , at 1028 ± 4 cm −1 , at 1085 ± 2 cm −1 , at 1325.5 ± 1.5 cm −1 , and at a range of 1412–1420 cm −1 , were observable.…”

“…Still, they were safe to standard human embryonic kidney cell lines up to 100 μg/ml. Abdel Fadeel et al 47 reported that, at concentrations from 0.01 to 100 μg/ml, the greenly synthesized TiO 2 NPs were nontoxic to breast adenocarcinoma cell and normal human skin fibroblast. Based on the positive results of the biocompatibility test, it can be concluded that PVA:SA:TiO 2 nanocomposite films studied here had tremendous potential for use as wound dressing materials.…”