Novel highly hydrophilic zwitterionic monolithic column for hydrophilic interaction chromatography

Jiang, Zhengjin; Smith, Norman W.; Ferguson, Paul; Taylor, M.R.S.

doi:10.1002/jssc.200900130

Cited by 45 publications

(35 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Figure 2 shows the fast separation of naphthalene, uracil and cytosine with 25 mM ammonium acetate, pH 5.0, in 85% ACN as the eluent. Baseline separation of these analytes was achieved in less than 15 s, which is much quicker than the 3 min separation obtained by Styros TM AminoHILIC column and the 50 s separation recently reported on a hydrophilic zwitterionic monolith [23]. However, the separation in Fig.…”

Section: Fast Separation Of Naphthalene Uracil and Cytosinementioning

confidence: 88%

“…However, grafted polymer monoliths have demonstrated improved efficiencies in the ion exchange mode [22] and increased crosslinking can reduce swelling for zwitterionic RP/HILIC monoliths [23]. HILIC columns have been prepared by dynamically coating silica particles with polyamines such as spermidine, putrescine and triethylene tetramine [16].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Agglomerated silica monolithic column for hydrophilic interaction LC

Ibrahim

Zhou

Lucy

2010

J of Separation Science

View full text Add to dashboard Cite

Hydrophilic interaction LC (HILIC) has gained wide acceptance in recent years due to its ability to retain and separate polar compounds such as pharmaceuticals. Most commercial HILIC phases are particle based, which limit the speed with which HILIC separations can be performed. Herein, agglomerated silica monolithic columns are prepared by electrostatically attaching polyionic latex particles onto a silica monolith by simply flushing a suspension of the ionic latex through a silica monolith. Such phases retain the high efficiency and permeability of the native silica monolith, while the agglomerated phase is easy to introduce and provides excellent mass transfer. High %ACN in the mobile phase dramatically increases the efficiency and retention, consistent with HILIC behavior. Test analytes such as benzoates, nucleotides and amino acids are separated with plate heights of 25-110 microm. The high permeability of monoliths allows HILIC separations to be performed with baseline resolution in less than 15 s. Electrostatic repulsion-hydrophilic liquid interaction chromatographic retention behavior of the latex-coated monoliths is verified using amino acids as test analytes.

show abstract

Section: Fast Separation Of Naphthalene Uracil and Cytosinementioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Agglomerated silica monolithic column for hydrophilic interaction LC

Ibrahim

Zhou

Lucy

2010

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Hydrophilic interaction chromatography (HILIC) is becoming increasingly popular [27][28][29][30] and has been successfully used for the separation of peptides [31,32] and of amino acids [33,34]. The growing interest in HILIC is partly due to its high solvent compatibility with MS in the electrospray ionization (ESI) mode [35,36].…”

Section: Introductionmentioning

confidence: 99%

Hydrophilic properties as a new contribution for computer-aided identification of short peptides in complex mixtures

et al. 2012

View full text Add to dashboard Cite

A new method to predict elementary amino acid (AA) composition of peptides (molar mass <1,000 g/mol) is described. This procedure is based on a computer-aided method using three combined analyses-reversed phase liquid chromatography (RPLC), hydrophilic interaction chromatography (HILIC) and capillary electrophoresis coupled with mass spectrometry-and using a software calculating all possible amino acid combinations from the mass of any given peptide. The complementarity between HILIC and RPLC was demonstrated. Peptide retention prediction in HILIC was successfully modelled, and the achieved prediction accuracy was as high as r²=0.97. This mathematical model, based on amino acid retention contributions and peptide length, provided the information about peptide hydrophilicity that was not redundant with its hydrophobicity. Correlations between respectively the hydrophobicity coefficients and RPLC retention time, hydrophilicity and HILIC retention time, and electrophoretic mobility and migration time were used for ranking all potential AA combinations corresponding to the given mass. The essential contribution of HILIC in this identification strategy and the need to combine the three models to significantly increase identification capabilities were both shown. Applied to an 18-standard peptide mixture, the identification procedure enabled the actual AA combination determination of the 14 di- to pentapeptides, in addition to an over 98 % reduction of possible combination numbers for the four hexapeptides. This procedure was then applied to the identification of 24 unknown peptides in a rapeseed protein hydrolysate. The effective AA composition was found for ten peptides, whereas for the 14 other peptides, the number of possible combinations was reduced by over 95 % thanks to the association of the three analyses. Finally, as a result of the information provided by the analytical techniques about peptides present in the mixture, the proposed method could become a highly valuable tool to recover bioactive peptides from undefined protein hydrolysates.

show abstract

“…More recently, monoliths with zwitterionic functional groups have also been developed as HILIC stationary phases since they take advantage of weak electrostatic interaction between charged analytes and zwitterionic functional groups, combined with high efficiency and selectivity [48,[82][83][84]. A novel kind of zwitterionic monolith was prepared by the copolymerization of 2-methacryloyloxyethyl phosphorylcholine and ethylene glycol dimethacrylate in a capillary for HILIC [84].…”

Section: Hydrophilic Interaction Chromatographymentioning

confidence: 99%

Recent advances in monolithic columns for protein and peptide separation by capillary liquid chromatography

Liang

Zhang

2012

Anal Bioanal Chem

View full text Add to dashboard Cite

Capillary liquid chromatography (cLC) has great potential for protein and peptide separation, with advantages of high efficiency, high resolution, low sample consumption, and high sensitivity when coupled with mass spectrometry. In recent years, monoliths have been widely used as the stationary phases for capillary columns, owing to easy preparation, high permeability, fast mass transfer, and low backpressure. This review summarizes recent advances (2007)(2008)(2009)(2010)(2011)(2012) in monolithic columns for protein and peptide separation by cLC. After a brief introduction on the preparation of monolithic capillary columns, the emphasis of this review is focused on the recent application of such columns for protein and peptide separation by cLC. Furthermore, the challenges and potential hot points of monolithic capillary columns in the future are discussed.

show abstract

Novel highly hydrophilic zwitterionic monolithic column for hydrophilic interaction chromatography

Cited by 45 publications

References 56 publications

Agglomerated silica monolithic column for hydrophilic interaction LC

Agglomerated silica monolithic column for hydrophilic interaction LC

Hydrophilic properties as a new contribution for computer-aided identification of short peptides in complex mixtures

Recent advances in monolithic columns for protein and peptide separation by capillary liquid chromatography

Contact Info

Product

Resources

About