Novel Hybrid Gels Made of High and Low Molecular Weight Hyaluronic Acid Induce Proliferation and Reduce Inflammation in an Osteoarthritis<i> In Vitro</i> Model Based on Human Synoviocytes and Chondrocytes

Stellavato, Antonietta; Vassallo, Valentina; Gatta, Annalisa La; Pirozzi, Anna Virginia Adriana; Rosa, Mario De; Balato, Giovanni; D’Addona, Alessio; Tirino, Virginia; Ruosi, Carlo; Schiraldi, Chiara

doi:10.1155/2019/4328219

Cited by 31 publications

(46 citation statements)

References 49 publications

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“…Indeed, the HA fragments, depending on their size, display different rheological properties and have been shown to either stimulate or inhibit inflammatory response in targeted cells and in diseased tissues, and to differently modulate the production of specific inflammatory mediators (5,6). In agreement with the notion of the size-and structure-dependent bioactivity of HA, hybrid preparations of low-and high-MW HA (7,8), crosslinked-HA (9) and hexadecylic-derivatized HA (10) show even further variations/ complexity in terms of pharmacodynamics as compared to the native, linear polymers. From the clinical point of view, according to a Cochrane Database Systematic Review analysis based on a significant number of controlled trials, it has been demonstrated that HA injections significantly reduce pain in knee osteoarthritis (11).…”

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confidence: 77%

Efficacy of a Treatment for Gonarthrosis Based on the Sequential Intra-Articular Injection of Linear and Cross-Linked Hyaluronic Acids

Barbieri

Capparucci

Mannello

et al. 2019

Muscle Ligaments and Tendons J

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Background. This study evaluates the clinical and biochemical effects of sequential intra-articular (IA) injections of linear (LHA) and cross-linked hyaluronic acid (CLHA) in patients with Gonarthrosis (GA). Methods. Thirty-nine (39) patients (age 64.89 ± 8.83) received first the LHA injection (0.8-1.2 MDa, 32mg/2ml) and after 1 week the CLHA (1.0 MDa and 2.0 MDa, 75mg/3ml) one; this round was repeated after 6 months. Clinical assessments-i.e. ultrasonography, visual analogic scale (VAS) for pain, range of motion (ROM) and Western Ontario and McMaster Universities Osteoarthritis (WOMAC) index-were performed at baseline and at specific time points up to 12 months. Relevant markers were determined in blood and synovial fluid (SF) at selected intervals. SF from patients with recurrent effusion was subjected to proteomic analysis. Results. This schedule improved joint pain and function, and promoted a reduction of inflammatory cytokines (IL-1β, IL-9 and IL-17) in plasma and SF; cartilage thickness increased at 12 months and the increase negatively correlated with the baseline levels of C-telopeptide of type II collagen (CTX-II). SF proteomic revealed that proteins associated with inflammation (Apolipoprotein A-1, α-1 antitrypsin and IgK) decreased, while the IL-1 inhibitor Transthyretin increased. Conclusions. This schedule represents an effective treatment whose benefits persist up to 12 months after baseline.

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confidence: 77%

Efficacy of a Treatment for Gonarthrosis Based on the Sequential Intra-Articular Injection of Linear and Cross-Linked Hyaluronic Acids

Barbieri

Capparucci

Mannello

et al. 2019

Muscle Ligaments and Tendons J

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“…Synoviocytes cells were isolated as previously reported [16,68] from three women OA patients (58,60, and 63 years old) undergoing the surgical procedure of knee joint replacement at Orthopedics and Traumatology Department of University Federico II of Naples. The patients gave informed consent and the procedures were approved by Internal Ethical Committee.…”

Section: In Vitro Cell Cultures and Glycosaminoglycan Treatmentsmentioning

confidence: 99%

Differential Secretome Profiling of Human Osteoarthritic Synoviocytes Treated with Biotechnological Unsulfated and Marine Sulfated Chondroitins

Russo

Vassallo

Stellavato

et al. 2020

IJMS

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Symptomatic slow-acting drugs (SYSADOA) are increasingly used as effective therapies for osteoarthritis, representing an attractive alternative to analgesics or non-steroidal anti-inflammatory drugs to relieve disease symptoms. Pharmaceutical preparations of chondroitin sulfate, derived from animal sources, alone or in combination with glucosamine sulfate, are widely recognized for their beneficial effect on osteoarthritis treatment. A growing interest has also been devoted to understanding the molecular mechanisms modulated by SYSADOA using -omic strategies, most of which rely on chondrocytes as a model system. In this work, by using an integrated strategy based on unbiased proteomics and targeted cytokine profiling by a multiplexed protein array, we identified differences in the secretomes of human osteoarthritic synoviocytes in response to biotechnological unsulfated, and marine sulfated chondroitins treatments. The combined strategy allowed the identification of candidate proteins showing both common and distinct regulation responses to the two treatments of chondroitins. These molecules, mainly belonging to ECM proteins, enzymes, enzymatic inhibitors and cytokines, are potentially correlated to treatment outcomes. Overall, the present results provide an integrated overview of protein changes in human osteoarthritic synoviocytes secretome associated to different chondroitin treatments, thus improving current knowledge of the biochemical effects driven by these drugs potentially involved in pathways associated to osteoarthritis pathogenesis.

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“…e bioactivity of HA in cell signaling, wound repair, morphogenesis, and matrix composition depends on its structure and biological properties [42,43]. Reviews assessing the promotion of the chondrocyte metabolism by HA [44] show that it can increase the synthetic amount of chondroitin-6-sulfate, collagen II, GAG, hydroxyproline, and DNA [45]. When delivering MSCs, HA-based hydrogels induce greater morphological differentiation of stem cells and help rebuild cartilage tissue.…”

Section: Ha-based Hydrogelsmentioning

confidence: 99%

“…When delivering MSCs, HA-based hydrogels induce greater morphological differentiation of stem cells and help rebuild cartilage tissue. Meanwhile, it also provides lubrication and buffering effects for recovering the viscosity and elasticity of synovia [44,46]. More importantly, through minimally invasive surgery, injectable HA-based hydrogels are capable of completely repairing small-scale bone and cartilage defects of any size.…”

Section: Ha-based Hydrogelsmentioning

confidence: 99%

The Application of Hyaluronic Acid-Based Hydrogels in Bone and Cartilage Tissue Engineering

Zheng

et al. 2019

Advances in Materials Science and Engineering

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At present, the healing of osteopathy, especially the healing of cartilage, has been proven to be difficult. Commonly used treatment methods are autogenous bone grafts and allogeneic bone grafts, but grafts cannot fully meet the clinical treatment requirements due to problems related to the source, price, immunity, and other concerns. us, the combination of biomaterials and tissue engineering technology has become a new direction in research. Among studies on tissue engineering bone and cartilage materials, hydrogels that show biological activity, absorbability after degradability, plasticity, and easy preparation have become the focus. Hydrogels are used as extracellular matrix mimics. Although various materials are able to form hydrogels, hyaluronic acid and its derivatives are prominently used. Hyaluronic acid hydrogels have many advantages, such as promoting cell adhesion and proliferation and wound healing. ey also demonstrate sufficient biological activity for stimulating a microenvironment for cell survival. However, their disadvantages require further modification and include a poor degradation rate and insufficient mechanical performance. In this paper, hyaluronic acid-based hydrogels, their modifications, applications, and mechanisms, as well as new techniques for processing hyaluronic acid hydrogels in bone and cartilage tissue engineering, are briefly reviewed, and their future prospects and directions for future work are discussed.

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Novel Hybrid Gels Made of High and Low Molecular Weight Hyaluronic Acid Induce Proliferation and Reduce Inflammation in an Osteoarthritis In Vitro Model Based on Human Synoviocytes and Chondrocytes

Cited by 31 publications

References 49 publications

Efficacy of a Treatment for Gonarthrosis Based on the Sequential Intra-Articular Injection of Linear and Cross-Linked Hyaluronic Acids

Efficacy of a Treatment for Gonarthrosis Based on the Sequential Intra-Articular Injection of Linear and Cross-Linked Hyaluronic Acids

Differential Secretome Profiling of Human Osteoarthritic Synoviocytes Treated with Biotechnological Unsulfated and Marine Sulfated Chondroitins

The Application of Hyaluronic Acid-Based Hydrogels in Bone and Cartilage Tissue Engineering

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