Novel hydrophilic nanostructured microtexture on direct metal laser sintered Ti-6Al-4V surfaces enhances osteoblast response<i>in vitro</i>and osseointegration in a rabbit model

Hyzy, Sharon L.; Cheng, Aijie; Cohen, David J.; Yatzkaier, G.; Whitehead, Alexander J.; Clohessy, Ryan M.; Gittens, Rolando A.; Boyan, Barbara D.; Schwartz, Zvi

doi:10.1002/jbm.a.35739

Cited by 65 publications

(53 citation statements)

References 56 publications

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“…Recent studies comparing porous constructs and solid implants, both with clinically relevant surface treatments showed comparable mechanical force to failure even though porous implants had bone ingrowth whereas solid implants had bone on‐growth (Cheng, Humayun, Boyan, & Schwartz, ; Cheng, Humayun, & Cohen, ; Hyzy et al, ). These earlier studies did not examine the effects of differing porous architecture while maintaining pore size and porosity.…”

Section: Discussionmentioning

confidence: 99%

“…However, for implants that depend on bone ingrowth for stability, there is still debate on the optimal architecture and pore properties for enhanced osseointegration. Porous implants support increased bone ingrowth, although a study comparing AM fabricated porous implants with solid implants in rabbit tibial bone defects and in a rat calvarial on‐lay model for vertical bone ingrowth, showed that the porous implants were comparable to their solid counterparts concerning pull out force to failure (Cheng, Cohen, et al, ; Hyzy et al, ). The specific properties of the pores can also affect outcomes.…”

Section: Introductionmentioning

confidence: 99%

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Hot isostatic pressure treatment of 3D printed Ti6Al4V alters surface modifications and cellular response

et al. 2019

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Additive manufacturing can be used to create personalized orthopedic and dental implants with varying geometries and porosities meant to mimic morphological properties of bone. These qualities can alleviate stress shielding and increase osseointegration through bone ingrowth, but at the expense of reduced fatigue properties compared to machined implants, and potential for loose build particle erosion. Hot isostatic pressure (HIP) treatment is used to increase fatigue resistance; implant surface treatments like grit‐blasting and acid‐etching create microroughness and reduce the presence of loose particles. However, it is not known how HIP treatment affects surface treatments and osseointegration of the implant to bone. We manufactured two titanium–aluminum–vanadium constructs, one with simple through‐and‐through porosity and one possessing complex trabecular bone‐like porosity. We observed HIP treatment varied in effect and was dependent on architecture. Micro/meso/nano surface properties generated by grit‐blasting and acid‐etching were altered on biomimetic HIP‐treated constructs. Human mesenchymal stem cells (MSCs) were cultured on constructs fabricated +/− HIP and subsequently surface‐treated. MSCs were sensitive to 3D‐architecture, exhibiting greater osteogenic differentiation on constructs with complex trabecular bone‐like porosity. HIP‐treatment did not alter the osteogenic response of MSCs to these constructs. Thus, HIP may provide mechanical and biological advantages during implant osseointegration and function.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hot isostatic pressure treatment of 3D printed Ti6Al4V alters surface modifications and cellular response

et al. 2019

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“…It is also possible that the range of periodicity of the line-like structures that were chosen for this study (3- surfaces with line-like structures with spatial periods smaller than 3 μm as well as larger than 20 μm. Previous studies demonstrated that roughing the surface of titanium seems to have the potential of promoting osseointegration (9,10).…”

Section: Discussionmentioning

confidence: 99%

Cytocompatibility of Direct Laser Interference-patterned Titanium Surfaces for Implants

Hartjen

Nada

Silva

et al. 2018

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Abstract. In an effort to generate titanium surfaces forimplants with improved osseointegration, we used direct laser interference patterning (DLIP) Improving the osseointegration of implants is a key issue in dental implantation and surface modification is a major strategy for enhancing the attachment, proliferation and differentiation of cells in contact with such implants.For titanium implants, various methods are used to modify the surface roughness, including sandblasting, acid-etching, anodization, calcium-phosphate crystal deposition and chemical modification (1).A recently developed technique for surface modification is direct laser interference patterning (DLIP) (2, 3). By overlapping two or more laser beams on a sample surface, periodic laser intensity distributions are obtained and used for laser ablation. DLIP is a very cost effective process for treating surfaces because it is possible to structure metals at high speeds of up to 0.39 m 2 /min (4). The structure periodicity can be controlled at the micrometer to submicrometer range and the surface roughness at the micrometer to nanometer level. Moreover, some chemical features can be modified, for example, the material's wetting properties (5).In the present study, we modified the surface of titanium of four different structures and assessed in vitro cytoxicity and cell attachment, as well as the viability and proliferation of cells cultured directly on the surfaces. Materials and MethodsTitanium specimens and reference materials. Line-like structures with spatial periods (p) of 3, 5, 10 and 20 μm were produced on pure titanium (grade IV) samples (Figure 1). All specimens were cylindrical with a diameter of 10 mm and thickness of 1.5 mm. Before and after laser treatment they were cleaned in pure ethanol (C 2 H 5 OH) using ultrasonic cleaning for 5 min each. As a reference surface, standard grit-blasted and etched samples (TiPure Plus; Bego Implant Systems, Bremen, Germany) were used. The surface characteristics of the DLIP-treated variants and TiPure Plus gritblasted and acid-etched reference material are shown in Table I.As a positive control for the in vitro cytocompatibility tests, RM-A, a polyurethane film sheet containing 0.1% zinc diethyldithio-carbamate (Hatano Research Institute, Hadano, Kanagawa, Japan) was used. As a negative control, Wako plastic sheets (cat. no. 160-08893; Wako Chemicals GmbH, Neuss, Germany) were used.

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“…Our group has shown that laser sintered Ti-6Al-4V solid implants placed in rabbit tibia cortical bone perform better than traditional implants manufactured with computer numerical control (CNC) [17]. We have also shown in previous studies that use of an osteoinductive agent (DBX, Musculoskeletal Transplant Foundation, Edison, New Jersey, USA) on rat calvaria did not alter the mechanical pull-out strength or new bone volume analyzed by microCT of porous laser sintered implants at 10 weeks postoperatively [13].…”

Section: Discussionmentioning

confidence: 99%

“…These results combined with in vivo studies demonstrating the ability of laser sintered implants to perform as well as implants manufactured with conventional techniques in animal models [17, 18], suggest that laser sintered implants with trabecular bone-inspired porosity may be superior to solid implants and enhance osseointegration in challenging clinical cases. While these results are promising, osseointegration of implants with trabecular bone-inspired porosity has not yet been analyzed in a clinically relevant orthopaedic or dental animal model.…”

Section: Introductionmentioning

confidence: 99%

Performance of laser sintered Ti–6Al–4V implants with bone-inspired porosity and micro/nanoscale surface roughness in the rabbit femur

et al. 2017

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Long term success of bone-interfacing implants remains a challenge in compromised patients and in areas of low bone quality. While surface roughness at the micro/nanoscale can promote osteogenesis, macro-scale porosity is important for promoting mechanical stability of the implant over time. Currently, machining techniques permit pores to be placed throughout the implant, but the pores are generally uniform in dimension. The advent of laser sintering provides a way to design and manufacture implants with specific porosity and variable dimensions at high resolution. This approach enables production of metal implants that mimic complex geometries found in biology. In this study, we used a rabbit femur model to compare osseointegration of laser sintered solid and porous implants. Ti-6Al-4V implants were laser sintered in a clinically relevant size and shape. One set of implants had a novel porosity based on human trabecular bone; both sets had grit-blasted/acid-etched surfaces. After characterization, implants were inserted transaxially into rabbit femora; mechanical testing, microCT and histomorphometry were conducted 10 weeks postoperatively. There were no differences in pull-out strength or bone-to-implant contact. However, both microCT and histomorphometry showed significantly higher new bone volume for porous compared to solid implants. Bone growth was observed into porous implant pores, especially near apical portions of the implant interfacing with cortical bone. These results show that laser sintered Ti-6Al-4V implants with micro/nanoscale surface roughness and trabecular bone-inspired porosity promote bone growth and may be used as a superior alternative to solid implants for bone-interfacing implants.

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Novel hydrophilic nanostructured microtexture on direct metal laser sintered Ti-6Al-4V surfaces enhances osteoblast responsein vitroand osseointegration in a rabbit model

Cited by 65 publications

References 56 publications

Hot isostatic pressure treatment of 3D printed Ti6Al4V alters surface modifications and cellular response

Hot isostatic pressure treatment of 3D printed Ti6Al4V alters surface modifications and cellular response

Cytocompatibility of Direct Laser Interference-patterned Titanium Surfaces for Implants

Performance of laser sintered Ti–6Al–4V implants with bone-inspired porosity and micro/nanoscale surface roughness in the rabbit femur

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