Novel immortalization approach defers senescence of cultured canine adipose-derived mesenchymal stromal cells

Stojiljković, Ana; Gaschen, Véronique; Forterre, Franck; Rytz, Ulrich; Stoffel, Michael Hubert; Bluteau, Jasmin

doi:10.1007/s11357-021-00488-x

Cited by 4 publications

(5 citation statements)

References 49 publications

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“…On the other hand, the immortalization of MSCs allows them to circumvent cellular senescence and to be expanded through unlimited cell passages in a genomically stable fashion without the tendency of cell malignancy or transformation. 354 HB1.F3 is a prominent example of an immortalized stem cell whose safety and potential for application in various cancer treatment approaches have been reported in several preclinical studies [355][356][357] and clinical trials (NCT02015819, NCT01172964). HB1.F3 is a human neural stem cell (NSC) line that is generated using the immortalization of stem cells isolated from 14-week fetal telencephalon tissue by a retroviral vector encoding v-Myc oncogene.…”

Section: Immortalization Of Characterized Therapeutic Mscs To Produce...mentioning

confidence: 99%

“…The immortalized cAT-MSCs retained their proliferation and differentiation capacity for a longer time in comparison to naive cAT-MSCs. 354 Shu et al have successfully immortalized UC MSCs using the SV40 T antigen immortalization system based on the PiggyBac transposon. These immortalized MSCs expressed MSC markers, had differentiation capacity, and no in vivo tumorigenicity.…”

Section: Immortalization Of Characterized Therapeutic Mscs To Produce...mentioning

confidence: 99%

“…Creating immortalized MSCs through genetic manipulation is a possible solution to bypass both MSC heterogeneity and limited expansion capacity issues. On the other hand, the immortalization of MSCs allows them to circumvent cellular senescence and to be expanded through unlimited cell passages in a genomically stable fashion without the tendency of cell malignancy or transformation 354 . HB1.F3 is a prominent example of an immortalized stem cell whose safety and potential for application in various cancer treatment approaches have been reported in several preclinical studies 355–357 and clinical trials (NCT02015819, NCT01172964).…”

Section: Promising Technologies That Can Help To Overcome the Challen...mentioning

confidence: 99%

“…Stojiljković et al have demonstrated the cAT‐MSCs immortalization using a PiggyBac™ Transposon System, which encodes the human polycomb ring finger proto‐oncogene B‐cell‐specific moloney murine leukemia virus integration site 1 and the human telomerase reverse transcriptase under the same promoter. The immortalized cAT‐MSCs retained their proliferation and differentiation capacity for a longer time in comparison to naive cAT‐MSCs 354 . Shu et al have successfully immortalized UC MSCs using the SV40 T antigen immortalization system based on the PiggyBac transposon.…”

Section: Promising Technologies That Can Help To Overcome the Challen...mentioning

confidence: 99%

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Nanotechnology and bioengineering approaches to improve the potency of mesenchymal stem cell as an off‐the‐shelf versatile tumor delivery vehicle

Taheri,

Tehrani,

Dehghani

et al. 2024

Medicinal Research Reviews

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Targeting actionable mutations in oncogene‐driven cancers and the evolution of immuno‐oncology are the two prominent revolutions that have influenced cancer treatment paradigms and caused the emergence of precision oncology. However, intertumoral and intratumoral heterogeneity are the main challenges in both fields of precision cancer treatment. In other words, finding a universal marker or pathway in patients suffering from a particular type of cancer is challenging. Therefore, targeting a single hallmark or pathway with a single targeted therapeutic will not be efficient for fighting against tumor heterogeneity. Mesenchymal stem cells (MSCs) possess favorable characteristics for cellular therapy, including their hypoimmune nature, inherent tumor‐tropism property, straightforward isolation, and multilineage differentiation potential. MSCs can be loaded with various chemotherapeutics and oncolytic viruses. The combination of these intrinsic features with the possibility of genetic manipulation makes them a versatile tumor delivery vehicle that can be used for in vivo selective tumor delivery of various chemotherapeutic and biological therapeutics. MSCs can be used as biofactory for the local production of chemical or biological anticancer agents at the tumor site. MSC‐mediated immunotherapy could facilitate the sustained release of immunotherapeutic agents specifically at the tumor site, and allow for the achievement of therapeutic concentrations without the need for repetitive systemic administration of high therapeutic doses. Despite the enthusiasm evoked by preclinical studies that used MSC in various cancer therapy approaches, the translation of MSCs into clinical applications has faced serious challenges. This manuscript, with a critical viewpoint, reviewed the preclinical and clinical studies that have evaluated MSCs as a selective tumor delivery tool in various cancer therapy approaches, including gene therapy, immunotherapy, and chemotherapy. Then, the novel nanotechnology and bioengineering approaches that can improve the potency of MSC for tumor targeting and overcoming challenges related to their low localization at the tumor sites are discussed.

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Section: Immortalization Of Characterized Therapeutic Mscs To Produce...mentioning

confidence: 99%

Section: Immortalization Of Characterized Therapeutic Mscs To Produce...mentioning

confidence: 99%

Section: Promising Technologies That Can Help To Overcome the Challen...mentioning

confidence: 99%

Section: Promising Technologies That Can Help To Overcome the Challen...mentioning

confidence: 99%

See 2 more Smart Citations

Nanotechnology and bioengineering approaches to improve the potency of mesenchymal stem cell as an off‐the‐shelf versatile tumor delivery vehicle

Taheri,

Tehrani,

Dehghani

et al. 2024

Medicinal Research Reviews

View full text Add to dashboard Cite

show abstract

“…Ana and colleagues reported one of the attempts to achieve MSC immortality. They designed a PiggyBac™ (PB) transposon system to simultaneously overexpress human polycomb ring finger proto-oncogene BMI1 and the human telomerase reverse transcriptase gene in MSC using a non-viral integration vector to achieve canine ADSC immortality and maintain the proliferation and differentiation potential of MSC for a longer period of time [ 108 ]. Lai et al created immortalized MSC by transfecting MYC-carrying lentiviral particles, and they suggested that the use of exosomes rather than cells as therapeutic agents could reduce the risk of lentiviral integration [ 109 ].…”

Section: Techniques For Improvement Of Exosome Productionmentioning

confidence: 99%

Current Strategies for Promoting the Large-scale Production of Exosomes

et al. 2023

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Exosomes, as nanoscale biological vesicles, have been shown to have great potential for biomedical applications. However, the low yield of exosomes limits their application. In this review, we focus on methods to increase exosome yield. Two main strategies are used to increase exosome production, one is based on genetic manipulation of the exosome biogenesis and release pathway, and the other is by pretreating parent cells, changing the culture method or adding different components to the medium. By applying these strategies, exosomes can be produced on a large scale to facilitate their practical application in the clinic.

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The other side of the coin: mesenchymal stromal cell immortalization beyond evasion of senescence

Lenz

Wink

2023

Human Cell

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Novel immortalization approach defers senescence of cultured canine adipose-derived mesenchymal stromal cells

Cited by 4 publications

References 49 publications

Nanotechnology and bioengineering approaches to improve the potency of mesenchymal stem cell as an off‐the‐shelf versatile tumor delivery vehicle

Nanotechnology and bioengineering approaches to improve the potency of mesenchymal stem cell as an off‐the‐shelf versatile tumor delivery vehicle

Current Strategies for Promoting the Large-scale Production of Exosomes

The other side of the coin: mesenchymal stromal cell immortalization beyond evasion of senescence

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