Novel In Situ Gelling Hydrogels Loaded with Recombinant Collagen Peptide Microspheres as a Slow‐Release System Induce Ectopic Bone Formation

Fahmy-Garcia, Shorouk; Mumcuoglu, Didem; Miguel, Laura De; Dieleman, Veerle; Witte-Bouma, Janneke; Eerden, Bram C. J. van der; Driel, Marjolein van; Eglin, David; Verhaar, Jan A N; Kluijtmans, Sebastiaan G. J. M.; Osch, Gerjo J.V.M. van; Farrell, Eric

doi:10.1002/adhm.201800507

Cited by 17 publications

(8 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S5), and the molar percentage of peptide graft was 18.5%. Collagen peptide (COL-P) is the product of enzymatic hydrolysis of collagen, which could promote cell proliferation, migration, and thus may accelerate the wound healing (40). Then, the peptide conformation in HA-P and GM-P polymers was investigated by circular dichroism (CD) analysis.…”

Section: Preparation and Characterization Of Glycopeptide Gm-p And Ha-pmentioning

confidence: 99%

ECM-mimetic immunomodulatory hydrogel for methicillin-resistant Staphylococcus aureus –infected chronic skin wound healing

et al. 2022

View full text Add to dashboard Cite

The treatment of difficult-to-heal wounds remains a substantial clinical challenge due to deteriorative tissue microenvironment including the loss of extracellular matrix (ECM), excessive inflammation, impaired angiogenesis, and bacterial infection. Inspired by the chemical components, fibrous structure, and biological function of natural ECM, antibacterial and tissue environment–responsive glycopeptide hybrid hydrogel was developed for chronic wound healing. The hydrogel can facilitate the cell proliferation and macrophage polarization to M2 phenotype, and show potent antibacterial efficacy against both Gram-negative and Gram-positive bacteria. Significantly, the glycopeptide hydrogel accelerated the reconstruction of methicillin-resistant Staphylococcus aureus (MRSA)–infected full-thickness diabetic and scalding skin by orchestrating a pro-regenerative response indicated by abundant M2-type macrophages, attenuated inflammation, and promoted angiogenesis. Collectively, ECM-mimetic and immunomodulatory glycopeptide hydrogel is a promising multifunctional dressing to reshape the damaged tissue environment without additional drugs, exogenous cytokines, or cells, providing an effective strategy for the repair and regeneration of chronic cutaneous wounds.

show abstract

Section: Preparation and Characterization Of Glycopeptide Gm-p And Ha-pmentioning

confidence: 99%

ECM-mimetic immunomodulatory hydrogel for methicillin-resistant Staphylococcus aureus –infected chronic skin wound healing

et al. 2022

View full text Add to dashboard Cite

show abstract

“…262 Hydrogels were able to release around 50% of the GF in 7 days, which was enough to attract MSCs to the disc ex vivo. These platforms are also potential candidates for the encapsulation of GFs for various functions, including the potentiation of MSC differentiation to intervertebral disk-like cells, 263 bone regeneration, 264 or for the delivery of antibodies into the eye. 265 Following this idea, Steele et al fabricated a hydrophobic HA derivative that can be cross-linked by poly(ethylene glycol)-block-poly(lactic acid) (PEG-PLA) nanoparticles through the self-assembly of both components.…”

Section: Formation Of Polysaccharide-basedmentioning

confidence: 99%

Recent Progress on Polysaccharide-Based Hydrogels for Controlled Delivery of Therapeutic Biomolecules

Rial-Hermida

Rey‐Rico

Blanco-Fernandez

et al. 2021

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

A plethora of applications using polysaccharides have been developed in recent years due to their availability as well as their frequent nontoxicity and biodegradability. These polymers are usually obtained from renewable sources or are byproducts of industrial processes, thus, their use is collaborative in waste management and shows promise for an enhanced sustainable circular economy. Regarding the development of novel delivery systems for biotherapeutics, the potential of polysaccharides is attractive for the previously mentioned properties and also for the possibility of chemical modification of their structures, their ability to form matrixes of diverse architectures and mechanical properties, as well as for their ability to maintain bioactivity following incorporation of the biomolecules into the matrix. Biotherapeutics, such as proteins, growth factors, gene vectors, enzymes, hormones, DNA/RNA, and antibodies are currently in use as major therapeutics in a wide range of pathologies. In the present review, we summarize recent progress in the development of polysaccharide-based hydrogels of diverse nature, alone or in combination with other polymers or drug delivery systems, which have been implemented in the delivery of biotherapeutics in the pharmaceutical and biomedical fields.

show abstract

“…Therefore, in collagen-based long-acting formulations for tissue engineering of wound healing, bone regeneration, and vascularization, fibers or hydrogels with moderate mechanical strength and high stability were often prepared by the freezing/thawing cycle method or chemical cross-linking such as EDC/NHS. − However, these collagen long-acting formulations exhibit issues of initial burst release etc . Thus strategies of conjugation with other polymers such as hyaluronic acid, heparin, or liposome are currently significantly investigated to enhance the mechanical strength, better protect protein/peptide therapeutics from hydrolysis or enzyme degradation, and better control the release kinetics of loaded drugs. ,− …”

Section: Protein-based Systemsmentioning

confidence: 99%

Sustained Release Systems for Delivery of Therapeutic Peptide/Protein

et al. 2021

View full text Add to dashboard Cite

Peptide/protein therapeutics have been significantly applied in the clinical treatment of various diseases such as cancer, diabetes, etc. owing to their high biocompatibility, specificity, and therapeutic efficacy. However, due to their immunogenicity, instability stemming from its complex tertiary and quaternary structure, vulnerability to enzyme degradation, and rapid renal clearance, the clinical application of protein/peptide therapeutics is significantly confined. Though nanotechnology has been demonstrated to prevent enzyme degradation of the protein therapeutics and thus enhance the half-life, issues such as initial burst release and uncontrollable release kinetics are still unsolved. Moreover, the traditional administration method results in poor patient compliance, limiting the clinical application of protein/peptide therapeutics. Exploiting the sustained-release formulations for more controllable delivery of protein/peptide therapeutics to decrease the frequency of injection and enhance patient compliance is thus greatly meaningful. In this review, we comprehensively summarize the substantial advancements of protein/peptide sustainedrelease systems in the past decades. In addition, the advantages and disadvantages of all these sustained-release systems in clinical application together with their future challenges are also discussed in this review.

show abstract

Novel In Situ Gelling Hydrogels Loaded with Recombinant Collagen Peptide Microspheres as a Slow‐Release System Induce Ectopic Bone Formation

Cited by 17 publications

References 80 publications

ECM-mimetic immunomodulatory hydrogel for methicillin-resistant Staphylococcus aureus –infected chronic skin wound healing

ECM-mimetic immunomodulatory hydrogel for methicillin-resistant Staphylococcus aureus –infected chronic skin wound healing

Recent Progress on Polysaccharide-Based Hydrogels for Controlled Delivery of Therapeutic Biomolecules

Sustained Release Systems for Delivery of Therapeutic Peptide/Protein

Contact Info

Product

Resources

About