2019
DOI: 10.1017/s0007114519001934
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Novel insights for SREBP-1 as a key transcription factor in regulating lipogenesis in a freshwater teleost, grass carp Ctenopharyngodon idella

Abstract: Disturbances in lipid metabolism are at the core of several health issues facing modern society, including fatty liver and obesity. The sterol regulatory element-binding protein 1 (SREBP-1) is one important transcription factor regulating lipid metabolism, but the relevant mechanism still remains unknown. The present study determined the transcriptional regulation of SREBP-1 and its target genes (including acetyl-CoA carboxylase α (accα), fatty acid synthase (fas) and stearoyl-CoA desaturase 1 (scd1)) in a fre… Show more

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Cited by 30 publications
(9 citation statements)
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“…To identify the phosphorylation sites of Beclin1 and AMPKα1, we constructed the vectors of Beclin1 and AMPKα1 expression according to our publication ( 46 ). The open reading frames (ORFs) of Beclin1 and AMPKα1 sequences were subcloned into the pcDNA3.1 (+) vector with the GFP-tag and the His-tag, respectively.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…To identify the phosphorylation sites of Beclin1 and AMPKα1, we constructed the vectors of Beclin1 and AMPKα1 expression according to our publication ( 46 ). The open reading frames (ORFs) of Beclin1 and AMPKα1 sequences were subcloned into the pcDNA3.1 (+) vector with the GFP-tag and the His-tag, respectively.…”
Section: Methodsmentioning
confidence: 99%
“…The siRNA sequences were given in Supplementary Table 3 . Cell transfections was operated based on the protocols ( 46 ). Cells were treated for 48h to investigate the potential mechanism that AMPK and Beclin1 mediated phosphorus-induced lipolysis and lipophagy.…”
Section: Methodsmentioning
confidence: 99%
“…Plasmid construction. We constructed SUMO1-6 × His, UBC9-6 × His and SREBP1-Flag plasmids based on the methods described previously (25) . Mutants with the K145R, K254R, K264R, K392R and K419R mutations were obtained from wild-type SREBP1 after the site-directed mutagenesis.…”
Section: Methodsmentioning
confidence: 99%
“…The promoter sequences and functional analyses of adipose triglyceride lipase (atgl) and very long-chain-specific acyl-CoA dehydrogenase (acadvl) were based on the methods described in our previous study. 24 The primers are provided in Table S5. The activities of promoters were assessed by a dual luciferase reporter assay system (#E1910, Promega).…”
Section: Ethicalmentioning
confidence: 99%