Novel insights into the antiproliferative effects and synergism of quercetin and menadione in human leukemia Jurkat T cells

Băran, Irina; Ionescu, Diana N.; Filippi, Alexandru; Mocanu, Maria-Magdalena; Iftime, Adrian; Babes, Ramona Madalina; Tofolean, Ioana Teodora; Irimia, Ruxandra; Goicea, Alexandru; Popescu, V. M.; Dimancea, Alexandru; Neagu, Andrei; Ganea, Constanţa

doi:10.1016/j.leukres.2014.04.010

Cited by 29 publications

(18 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The ratio of Bcl-2/Bax determines survival or death following an apoptotic stimulus (Oltvai et al, 1993). Here, a significant decrease in Bcl-2/Bax ratio in K562 cells after PC-EEF treatment was detected, in accordance with the results obtained from the research of quercetin (Baran et al, 2014).…”

Section: Discussionsupporting

confidence: 88%

Polytrichum commune L.ex Hedw ethyl acetate extract-triggered perturbations in intracellular Ca2+ homeostasis regulates mitochondrial-dependent apoptosis

Yuan

Cheng

Wang

et al. 2015

Journal of Ethnopharmacology

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 88%

Polytrichum commune L.ex Hedw ethyl acetate extract-triggered perturbations in intracellular Ca2+ homeostasis regulates mitochondrial-dependent apoptosis

Yuan

Cheng

Wang

et al. 2015

Journal of Ethnopharmacology

View full text Add to dashboard Cite

“…In addition, MD proved to be effective against multidrug resistant and parental leukemia cell lines and also against DOX-resistant rat leukemia cells [25,26]. Interestingly, MD has synergistic cytotoxic effects in combination with vitamin C or with flavonoids quercetin and epigallocatechin-3-gallate in leukemia Jurkat cells [23,27,28].…”

Section: Introductionmentioning

confidence: 99%

Simple discrimination of sub-cycling cells by propidium iodide flow cytometric assay in Jurkat cell samples with extensive DNA fragmentation

Babes¹,

Tofolean²,

Sandu

et al. 2018

Cell Cycle

Self Cite

View full text Add to dashboard Cite

Human leukemia Jurkat T cells were analyzed for apoptosis and cell cycle by flow cytometry, using the Annexin V/propidium iodide (PI) standard assay, and a simple PI staining in Triton X-100/digitonin-enriched PI/RNase buffer, respectively. Cells treated with doxorubicin or menadione displayed a very strong correlation between the apoptotic cell fraction measured by the Annexin V/PI assay, and the weight of a secondary cell population that emerged on the forward scatter (FS)/PI plot, as well as on the side scatter (SS)/PI and FL1/PI plots generated from parallel cell cycle recordings. In both cases, the Pearson correlation coefficients were >0.99. In cell cycle determinations, PI fluorescence was detected on FL3 (620/30 nm), and control samples exhibited the expected linear dependence of FL3 on FL1 (525/40 nm) signals. However, increasing doses of doxorubicin or menadione generated a growing subpopulation of cells displaying a definite right-shift on the FS/FL3, SS/FL3 and FL1/FL3 plots, as well as decreased PI fluorescence, indicative of ongoing fragmentation and loss of nuclear DNA. By gating on these events, the resulting fraction of presumably sub-cycling cells (i.e. cells with cleaved DNA, counting sub-G/G, sub-S and sub-G/M cells altogether) was closely similar to the apoptotic rate assessed by Annexin V/PI labeling. Taken together, these findings suggest a possible way to recognize the entire population of cells undergoing apoptotic DNA cleavage and simultaneously determine the cell cycle distribution of non-apoptotic cells in PI-labeled cell samples with various degrees of DNA fragmentation, using a simple and reproducible multiparametric analysis of flow cytometric recordings.

show abstract

“…Вікасол здебільшого використовується як токсикант у моделюванні оксидаційного стресу [11,33]. За останні роки дослідження фармакологічних властивостей хінонів значно активізувались [3,8,11,13,24,31], хоча їх вплив на жирнокислотний склад тканин вивчено не достатньо. Описано вплив вікасолу на процеси транскрипції та активації чинників, які задіяні у ланцюгу антиоксидантної відповіді клітини.…”

Section: біохіміяunclassified

Жирнокислотний Склад Міокарду Гусей За Дії Вікасолу

Яковійчук¹,

Данченко²,

Danchenko³

et al. 2019

TernopilBioSciIssues

View full text Add to dashboard Cite

In science vicasol is known to be relative to the energy and antioxidant systems of tissues closely related to the biosynthesis and oxidation of fatty acids. This effect may cause changes in the fatty acid composition of tissues, and numerous works on the positive effect of quinones and their derivatives on the myocardial function, suggest that the proper dose and feeding can increase the stability and productivity of poultry. The given was aims to study the effect of vicasol on the fatty acid composition of goose myocardium. Myocardium was chosen as a biological object. Biological material was collected every 7 days throughout the period from the 21st to the 35th day of ontogeny, characterized by the state of physiological tension of geese. Feeding of geese with vicasol at a dose of 0.7 mg / kg body weight began with the 3rd day of ontogeny. Fatty acid analysis in myocardial tissues was performed by gas-liquid chromatography, pre-fabric samples were processed with the method by Palmer (1971) to obtain tissue lipid extracts. According to the results of the study, due to various changes in the content of the entire spectrum of fatty acids of the tissue during the experiment - the use of vicasol causes a slight increase in the unsaturation and the total content of unsaturated fatty acids in the myocardium of geese. These fluctuations are realized depending on the physiological state of the body. where vicasol can stimulate both the biosynthesis processes of individual fatty acids and their mitochondrial and microsomal oxidation, as evidenced by multidirectional reliable changes in the content of their entire spectrum. In particular, on the 21st day, the content of docosopentaenoic acid increased by 36.3% whereas the content of docosohexaenoic and linolenic acids decreased by an average of 21–24%, on the 28th day the content of eicosatetraic and docosahexaenoic acids increased whereas the content of the linoleic acids dropped by 22.6% in control groups. On the 35th day, the content of basic unsaturated fatty acids: palmitooleic, linoleic, linolenic and docosohexaenoic acids increased in the tissue under the influence of vicasol with complete depletion of docosopentaenoic acid. These fluctuations in fatty acid composition cause a slight increase in the total content of unsaturated fatty acids and increase the unsaturation of myocardial lipids on the 28th and 35th days of ontogeny of geese. Based on previous results regarding the antioxidant state of myocardium affected by vicasol and the given findings, which prove changes in the content of the entire spectrum of fatty acids during the selected ontogeny, vicasol can be used in poultry farming as a tool to improve the quality and the resilience of poultry.

show abstract

Novel insights into the antiproliferative effects and synergism of quercetin and menadione in human leukemia Jurkat T cells

Cited by 29 publications

References 66 publications

Polytrichum commune L.ex Hedw ethyl acetate extract-triggered perturbations in intracellular Ca2+ homeostasis regulates mitochondrial-dependent apoptosis

Polytrichum commune L.ex Hedw ethyl acetate extract-triggered perturbations in intracellular Ca2+ homeostasis regulates mitochondrial-dependent apoptosis

Simple discrimination of sub-cycling cells by propidium iodide flow cytometric assay in Jurkat cell samples with extensive DNA fragmentation

Жирнокислотний Склад Міокарду Гусей За Дії Вікасолу

Contact Info

Product

Resources

About