Novel insights into the fruit and seed extracts ofMorinda morindoides(Baker) Milne‐Redh: HPLC‐ESI‐Q‐TOF‐MS profiling, antioxidant, and enzyme inhibitory propensities

Abstract

In this study, we attempted for the first time to determine the phytochemical compositions and biopharmaceutical properties of the methanolic and aqueous extracts of Morinda morindoides fruits and seeds. Antioxidant propensities of the extracts were determined via free radical‐scavenging, phosphomolybdenum, metal chelating, and reducing power assays. Inhibitory activities against cholinesterases (acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)), tyrosinase, α‐amylase, and α‐glucosidase were assessed. Moreover, the total phenolic and flavonoid contents were determined. High Performance Liquid Chromatography coupled to Electrospray Ionization Quadrupole Time‐of‐Flight Mass Spectrometry (HPLC‐ESI‐Q‐TOF‐MS) analysis was performed to establish the chemical profile of the investigated extracts. The tested extracts showed remarkable antioxidant abilities with aqueous fruit extract being most potent in all assays, except for ferrous ion chelating assay. Overall, the methanolic extracts were potent inhibitors of the tested enzymes. In conclusion, M. morindoides may be endorsed as promising sources of bioactive compounds and used for designing functional foods. Practical applications In recent years, new compounds from plant sources have attracted great interest to design functional foods. Thus, we aimed to detect biological and chemical properties of Morinda morindoides fruits and seeds, a widely used as food with medicinal properties in Africa. Based on our findings, fruits extracts exhibited stronger activity when compared to seed extracts. Also, chemical identification was performed for each extracts. The presented findings could provide valuable information into the pool of scientific knowledge and may be basis for the design of novel functional applications from M. morindoides.

Biological properties and HPLC analyses of four medicinal plants extracts obtained at different extraction temperatures

“…, whereby the highest anti-tyrosinase effect was found in the root methanol extract with IC 50 of 3.55 ± 0.35 mg/ml compared to other tested extracts of C. cajan. Besides, in one of our previous work, the methanolic extracts of M. morindoides was noted to have remarkable inhibitory propensities the enzymes tested in the current study, including tyrosinase(Sinan et al, 2020). Kim Lien et al (2019) also reported iso-…”

“…Besides, in one of our previous work, the methanolic extracts of M . morindoides was noted to have remarkable inhibitory propensities the enzymes tested in the current study, including tyrosinase (Sinan et al., 2020). Kim Lien et al.…”