Novel insights into the somatic proteome of Strongyloides stercoralis infective third-stage larvae

Dishnica, Klevia; Piubelli, Chiara; Manfredi, Marcello; Kondaveeti, Ravi Teja; Longoni, Silvia Stefánia; Degani, Monica; Buonfrate, Dora; Giorgetti, Alejandro; Tiberti, Natalia

doi:10.1186/s13071-023-05675-7

Cited by 4 publications

(18 citation statements)

References 54 publications

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“…Seven protein families have been identified by other studies as expanded in the genomes of parasitic nematodes, and upregulated in parasitic life stages [38]. Studies of the Ancylostoma hookworm E/S proteome and S. venezuelensis and S. stercoralis somatic larval proteomes detected some of the same families (SCP/TAPS, proteases and TTL) indicating possible presence in stool [70][71][72]. However, specific proteins within these families must still be selected as candidate antigens because every individual protein is not expressed simultaneously.…”

Section: Discussionmentioning

confidence: 92%

“…A similar study by Culma (2021) investigated cellular location, antigenicity and allergenicity, among other features, in the S. stercoralis proteome to identify potential vaccine and diagnostic targets [69]. Recently, Dishnica et al (2023) [70] published the somatic proteome of S. stercoralis iL3 isolated from a clinical case and conducted a similar computational pipeline to discover possible novel serological antigens. While their main candidates do not overlap with those from the present study, three appear in the DE dataset (S2 File) as they were differentially expressed more highly in non-gut-dwelling life stages, predominantly iL3, as would be expected (Q9UA16/ SSTP_0001008900, A0A0K0E2F4/SSTP_0000367400, A0A0K0DTP5/SSTP_0000060800), and three proteins were present in the E/S orthologues (A0A0K0E6J0/SSTP_0000511900, Q9UA16/SSTP_0001008900, A0A0K0DTP5/SSTP_0000060800.…”

Section: Discussionmentioning

confidence: 99%

“…stercoralis proteome to identify potential vaccine and diagnostic targets [ 69 ]. Recently, Dishnica et al (2023) [ 70 ] published the somatic proteome of S . stercoralis iL3 isolated from a clinical case and conducted a similar computational pipeline to discover possible novel serological antigens.…”

Section: Discussionmentioning

confidence: 99%

“…Possible data sources for further antigen searches continue to be added to and include: an analysis of E/S proteomes by Tritten et al (2021) [75]; analysis of S. venezuelensis somatic proteins by Fonseca et al (2019) [71], and S. venezuelensis E/S proteins by Maeda et al (2019) [102]; also S. stercoralis iL3 somatic proteins by Dishnica et al (2023) [70]. Somatic protein datasets would include tegument antigens as might be present in stool if whole or parts of larvae were present, or if the proteins were secreted via non-classical pathways, as proposed for some S. venezuelensis proteins [102].…”

Section: Limitations and Future Workmentioning

confidence: 99%

“…This has impacted on diagnosis and vaccination for other parasitic infections [109,110]. Diversity can be readily investigated by amplifying and sequencing the genes of interest from a wide geographic range of samples, or investigating differences between actual protein sequences from clinical isolates [70] and their predicted counterpart from genomes. For S. stercoralis, this is especially required, because the reference genome strain PV001 originates from a dog infection with UPD (University of Pennsylvania dog) strain [111].…”

Section: Limitations and Future Workmentioning

confidence: 99%

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A comparative ‘omics’ approach for prediction of candidate Strongyloides stercoralis diagnostic coproantigens

et al. 2023

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Human infection with the intestinal nematode Strongyloides stercoralis is persistent unless effectively treated, and potentially fatal in immunosuppressed individuals. Epidemiological data are lacking, partially due to inadequate diagnosis. A rapid antigen detection test is a priority for population surveillance, validating cure after treatment, and for screening prior to immunosuppression. We used a targeted analysis of open access ‘omics’ data sets and used online predictors to identify S. stercoralis proteins that are predicted to be present in infected stool, Strongyloides-specific, and antigenic. Transcriptomic data from gut and non-gut dwelling life cycle stages of S. stercoralis revealed 328 proteins that are differentially expressed. Strongyloides ratti proteomic data for excreted and secreted (E/S) proteins were matched to S. stercoralis, giving 1,057 orthologues. Five parasitism-associated protein families (SCP/TAPS, prolyl oligopeptidase, transthyretin-like, aspartic peptidase, acetylcholinesterase) were compared phylogenetically between S. stercoralis and outgroups, and proteins with least homology to the outgroups were selected. Proteins that overlapped between the transcriptomic and proteomic datasets were analysed by multiple sequence alignment, epitope prediction and 3D structure modelling to reveal S. stercoralis candidate peptide/protein coproantigens. We describe 22 candidates from seven genes, across all five protein families for further investigation as potential S. stercoralis diagnostic coproantigens, identified using open access data and freely-available protein analysis tools. This powerful approach can be applied to many parasitic infections with ‘omic’ data to accelerate development of specific diagnostic assays for laboratory or point-of-care field application.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Limitations and Future Workmentioning

confidence: 99%

Section: Limitations and Future Workmentioning

confidence: 99%

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A comparative ‘omics’ approach for prediction of candidate Strongyloides stercoralis diagnostic coproantigens

et al. 2023

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Advancing Strongyloides omics data: bridging the gap with Caenorhabditis elegans

Al-Jawabreh,

Lastik,

McKenzie

et al. 2023

Phil. Trans. R. Soc. B

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Among nematodes, the free-living model organismCaenorhabditis elegansboasts the most advanced portfolio of high-quality omics data. The resources available for parasitic nematodes, includingStrongyloidesspp., however, are lagging behind. WhileC. elegansremains the most tractable nematode and has significantly advanced our understanding of many facets of nematode biology,C. elegansis not suitable as a surrogate system for the study of parasitism and it is important that we improve the omics resources available for parasitic nematode species. Here, we review the omics data available forStrongyloidesspp.and compare the available resources to those forC. elegansand other parasitic nematodes. The advancements inC. elegansomics offer a blueprint for improving omics-led research inStrongyloides. We suggest areas of priority for future research that will pave the way for expansions in omics resources and technologies.This article is part of the Theo Murphy meeting issue ‘Strongyloides: omics to worm-free populations’.

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Progresses and challenges in Strongyloides spp. proteomics

Tiberti,

Manfredi,

Piubelli

et al. 2023

Phil. Trans. R. Soc. B

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The availability of high-quality data of helminth genomes provided over the past two decades has supported and accelerated large-scale ‘omics studies and, consequently, the achievement of a more in-depth molecular characterization of a number of pathogens. This has also involved Strongyloides spp. and since their genome was made available transcriptomics has been rather frequently applied to investigate gene expression regulation across their life cycle. Strongyloides proteomics characterization has instead been somehow neglected, with only a few reports performing high-throughput or targeted analyses associated with protein identification by tandem mass spectrometry. Such investigations are however necessary in order to discern important aspects associated with human strongyloidiasis, including understanding parasite biology and the mechanisms of host–parasite interaction, but also to identify novel diagnostic and therapeutic targets. In this review article, we will give an overview of the published proteomics studies investigating strongyloidiasis at different levels, spanning from the characterization of the somatic proteome and excretory/secretory products of different parasite stages to the investigation of potentially immunogenic proteins. Moreover, in the effort to try to start filling the current gap in host-proteomics, we will also present the first serum proteomics analysis in patients suffering from human strongyloidiasis. This article is part of the Theo Murphy meeting issue ‘ Strongyloides : omics to worm-free populations’.

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Novel insights into the somatic proteome of Strongyloides stercoralis infective third-stage larvae

Cited by 4 publications

References 54 publications

A comparative ‘omics’ approach for prediction of candidate Strongyloides stercoralis diagnostic coproantigens

A comparative ‘omics’ approach for prediction of candidate Strongyloides stercoralis diagnostic coproantigens

Advancing Strongyloides omics data: bridging the gap with Caenorhabditis elegans

Progresses and challenges in Strongyloides spp. proteomics

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