Novel lactic acid bacteria inhibitingPaenibacillus larvaein honey bee larvae

Abstract

We evaluated the antagonistic effects of newly identified lactic acid bacteria (LAB) in the genera Lactobacillus and Bifidobacterium, originating from the honey stomach, on the honey bee pathogen, Paenibacillus larvae. We used inhibition assays on agar plates and honey bee larval bioassays to investigate the effects of honey bee LAB on P. larvae growth in vitro and on AFB infection in vivo. The individual LAB phylotypes showed different inhibition properties against P. larvae growth on agar plates, whereas a combination of all eleven LAB phylotypes resulted in a total inhibition (no visible growth) of P. larvae. Adding the LAB mixture to the larval food significantly reduced the number of AFB infected larvae in exposure bioassays. The results demonstrate that honey bee specific LAB possess beneficial properties for honey bee health. Possible benefits to honey bee health by enhancing growth of LAB or by applying LAB to honey bee colonies should be further investigated.

Isolation, Characterization and Identification of Probiotic Lactic Acid Bacteria (LAB) from Honey Bees

“…Honey bees possess a diverse LAB microbiota in their honey crop, acquired by consuming pollen and nectar and through contact with older bees of the colony. All the extant honey bee species possess Lactobacillus and Bifidobacterium within the crop in biofilms (Vasquez et al, 2012), with potential to inhibit American Foul Brood-causing Paenibacillus larvae growth on agar plate (Forsgren et al, 2009). Especially, Lactobacullus kunkeei is predominantly found in A. laboriosa honey in Nepal (10 8 cfu per gram honey), followed by A. mellifera honey in Africa.…”

Plasticity in the gut microbial community and uptake of Enterobacteriaceae (Gammaproteobacteria) in Bombus terrestris bumblebees’ nests when reared indoors and moved to an outdoor environment

“…set,^and the latter are generally found in the gut microbiota of social bees (Kaltenpoth 2011;Koch and Schmid-Hempel 2011b;Cariveau et al 2014). Core bacteria dominating the gut of social honeybees and bumblebees have been implicated in beneficial functions such as digestion and protection (Klungness and Peng 1984;Forsgren et al 2010;Vásquez et al 2012;Kwong et al 2014). Multiple conditions have been reported influencing the bacterial gut community, including the environment, host age, habitat, pathogens, and diet (Dillon et al 2010;Evans and Schwarz 2011;Hu et al 2014;Yun et al 2014).…”

Effects of Lactobacillus Johnsonii AJ5 Metabolites on Nutrition, Nosema Ceranae Development and Performance of Apis Mellifera L.

“…In this regard, some bacterial strains and products of their metabolism have begun to receive special scientific attention. Some studies have reported beneficial effects of microbiota on bees' health (Mattila et al, 2012;Janashia & Alaux, 2016) and undesirable effects particularly on microbial threats (Forsgren et al, 2009;Vásquez et al, 2012;Baffoni et al, 2015;Audisio, 2016). Our study was focused on assessing the effect of bacterial metabolites produced by a LAB (L. johnsonii AJ5) on nutritional parameters, N. ceranae development and performance of A. mellifera colonies.…”

“…There is strong evidence of an increase of antimicrobial peptide (AMPs) production induced by honey bee gut bacteria (Evans & Lopez, 2004;Jefferson et al, 2013;Yoshiyama et al 2013;Janashia & Alaux, 2016). Several in vitro trials confirmed the hability of lactobacilli and bifidobacteria to inhibit honey bee pathogens, in particular Paenibacillus larvae, Melissococcus plutonius and Ascosphaera apis (Forsgren et al, 2009;Sabaté et al, 2009;Yoshiyama & Kimura, 2009;Vásquez et al, 2012). Similarly, a reduction in N. ceranae intensity was reported when a Bacillus spp.…”