Novel lipase from basidiomycetes Schizophyllum commune ISTL04, produced by solid state fermentation of Leucaena leucocephala seeds

Singh, Manoj Kumar; Singh, Jaswant; Kumar, Madan; Thakur, Indu Shekhar

doi:10.1016/j.molcatb.2014.10.010

Cited by 36 publications

(17 citation statements)

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“…These results indicated that S. commune lipase did not precipitate after 80% of (NH 4 ) 2 SO 4 saturation. This is in agreement with the research done by Singh et al [3]. They precipitated the lipase of S. commune ISTL04 up to 70% (NH 4 ) 2 SO 4 saturation, before further purifying it by Superdex TM 200 gel permeation chromatography.…”

Section: Resultssupporting

confidence: 92%

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One-Step Partially Purified Lipases (ScLipA and ScLipB) from Schizophyllum commune UTARA1 Obtained via Solid State Fermentation and Their Applications

2017

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Lipases with unique characteristics are of value in industrial applications, especially those targeting cost-effectiveness and less downstream processes. The aims of this research were to: (i) optimize the fermentation parameters via solid state fermentation (SSF); and (ii) study the performance in hydrolysis and esterification processes of the one-step partially purified Schizophyllum commune UTARA1 lipases. Lipase was produced by cultivating S. commune UTARA1 on sugarcane bagasse (SB) with used cooking oil (UCO) via SSF and its production was optimized using Design-Expert® 7.0.0. Fractions 30% (ScLipA) and 70% (ScLipB) which contained high lipase activity were obtained by stepwise (NH4)2SO4 precipitation. Crude fish oil, coconut oil and butter were used to investigate the lipase hydrolysis capabilities by a free glycerol assay. Results showed that ScLipA has affinities for long, medium and short chain triglycerides, as all the oils investigated were degraded, whereas ScLipB has affinities for long chain triglycerides as it only degrades crude fish oil. During esterification, ScLipA was able to synthesize trilaurin and triacetin. Conversely, ScLipB was specific towards the formation of 2-mono-olein and triacetin. From the results obtained, it was determined that ScLipA and ScLipB are sn-2 regioselective lipases. Hence, the one-step partial purification strategy proved to be feasible for partial purification of S. commune UTARA1 lipases that has potential use in industrial applications.

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Section: Resultssupporting

confidence: 92%

“…However, it is also found to degrade wood and caused severe infections in humans [2]. Recently, Singh et al [3] has purified the lipase produced by S. commune which was cultivated on Leucaena leucocephala seeds under solid state fermentation conditions. Due to these findings, S. commune was selected for this study.…”

Section: Introductionmentioning

confidence: 99%

One-Step Partially Purified Lipases (ScLipA and ScLipB) from Schizophyllum commune UTARA1 Obtained via Solid State Fermentation and Their Applications

2017

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“…Enzyme lipase was partially purified according to the method described by Singh et al [15]. Briefly, 1 mmol dm −3 of phenylmethylsulfonyl fluoride (PMSF) was added into the crude enzyme extract in order to prevent enzyme degradation during the purification process.…”

Section: Enzyme Purificationmentioning

confidence: 99%

“…Many papers on lipase production using solid-state cultivation of fungi on different low-cost media derived from agroindustry residues have been published thus far, although no data of its successful commercialization on a large scale are available. With the purpose of lipase production, Aspergillus niger was cultivated on a variety of agricultural wastes [6][7][8][9], Rhizopus oryzae on sugarcane bagasse [10], Aspergillus species on olive pomace [11,12], Trichoderma strains on oil-palm fruit-bunch medium [13], Penicillium camemberti KCCM 11268 on wheat bran [14], Schizophyllum commune ISTL04 on Leucaena leucocephala seeds [15], etc.…”

Section: Introductionmentioning

confidence: 99%

Lipase Production by Solid-State Cultivation of Thermomyces Lanuginosus on By-Products from Cold-Pressing Oil Production

et al. 2019

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This study shows that by-products obtained after cold-pressing oil production (flex oil cake, hemp oil cake, hull-less pumpkin oil cake) could be used as substrates for the sustainable and cost-effective production of lipase when cultivating Thermomyces lanuginosus under solid-state conditions (T = 45 °C, t = 9 days). Lipase showed optimum activity at T = 40 °C. The produced lipase extract was purified 17.03-folds with a recovery of 1% after gel chromatography. Three different batch experiments were performed in order to test the possibility of using the lipase in biodiesel production. Experiments were performed with a commercial, unpurified enzyme, and partially purified lipase with sunflower oil and methanol as substrates in a batch reactor at 40 °C. During the experiments, the operational stability of the enzyme was studied. The obtained results clearly showed that produced crude and purified lipase can be used for biodiesel production, but the process needs some additional optimization. As for operation stability, it was noticed that the commercial enzyme was deactivated after 30 h, while produced crude enzyme remained 8.25% of its activity after 368 h.

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“…), так и у почвенных сапрофитов (Agaricus bisporus (J.E. Lange) Imbach), однако их активность и условия продуцирования исследованы недостаточно [10,20,22].…”

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Закономерности Биосинтеза Липолитических Ферментов Ксилотрофным Базидиомицетом Fomes Fomentarius

Almyasheva¹,

Golyshkin²

2016

СПНО (MPSE)

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Ксилотрофный базидиомицет Fomes fomentarius (L.) Fr. известен как продуцент широкого спектра окислительных и гидролитических ферментов, отвечающих за расщепление лигноцеллюлозного комплекса. В результате работы была установлена способность грибов данного вида продуцировать липолитические ферменты, находящие в современной биотехнологии все более широкое применение. Из 6 штаммов, выделенных с различных природных субстратов, был отобран F. fomentarius 12.13 как наиболее перспективный продуцент. Исследование липолитической активности поверхностной и погруженной культур базидиомицета показало, что наличие в питательной среде таких субстратов, как сусло, молочная сыворотка и пептон способствует активному биосинтезу липаз. Максимальная активность внеклеточных ферментов была получена через 120 ч погруженного культивирования и составила 107,1 мU/мл культуральной жидкости, внутриклеточных ферментов -4,9 мU/мг мицелия через 144 ч. Ключевые слова: Fomes fomentarius, липаза, базидиомицеты, погруженное культивирование BIOSYNTHESYS OF LIPOLYTIC ENZYMES BY XYLOTROPHIC BASIDIOMYCETE FOMES FOMENTARIUSAlmyasheva N.R. 1 , Golyshkin A.V. Federal State Budgetary Educational Institution of Higher Education «Gubkin Russian State University of Oil and Gas (National Research University)», Moscow, e-mail: almyashevanelya@mail.ruXylotrophic basidiomycete Fomes fomentarius (L.) Fr is known as a producer of a broad spectrum of oxidative and hydrolytic enzymes responsible for the degradation of the lignocellulosic complex. The study found that fungi of this species can produce lipolytic enzymes, which are widely used in biotechnology. Fomes fomentarius 12.13 was selected from 6 strains isolated from different natural substrates as the most promising producer. The study of lipolytic activity in submerged and surface cultures of basidiomycete showed that the presence of malt extract, milk serum and peptone in the medium promotes the lipase biosynthesis. The maximum exocellular enzyme activity was obtained after 120 h of submerged cultivation and amounted to 107.1 mU/ml, endocellular enzyme activity -4.9 mU/ml after 144 h.

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Novel lipase from basidiomycetes Schizophyllum commune ISTL04, produced by solid state fermentation of Leucaena leucocephala seeds

Cited by 36 publications

References 50 publications

One-Step Partially Purified Lipases (ScLipA and ScLipB) from Schizophyllum commune UTARA1 Obtained via Solid State Fermentation and Their Applications

One-Step Partially Purified Lipases (ScLipA and ScLipB) from Schizophyllum commune UTARA1 Obtained via Solid State Fermentation and Their Applications

Lipase Production by Solid-State Cultivation of Thermomyces Lanuginosus on By-Products from Cold-Pressing Oil Production

Закономерности Биосинтеза Липолитических Ферментов Ксилотрофным Базидиомицетом Fomes Fomentarius

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