Novel members of the family Micromonosporaceae, Rugosimonospora acidiphila gen. nov., sp. nov. and Rugosimonospora africana sp. nov.

Monciardini, Paolo; Cavaletti, Linda; Ranghetti, Anna; Schümann, Peter; Rohde, Manfred; Bamonte, Ruggiero; Sosio, Margherita; Mezzelani, Alessandra; Donadio, Stefano

doi:10.1099/ijs.0.010231-0

Cited by 31 publications

(13 citation statements)

References 30 publications

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“…Since the isolation of acidophilic streptomycetes from acidic soil samples by Williams and colleagues (15,16), studies of acidophilic actinomycetes have been focused mainly on the description of novel taxa and on their phylogenetic diversity (17)(18)(19)(20)(21)(22). Acidophilic actinomycetes can be assigned to two groups, namely, a group of moderately acidophilic actinomycetes with an optimal pH of around 6.0 and a group of obligately acidophilic actinomycetes with an optimal pH of no more than 4.0 (23).…”

mentioning

confidence: 99%

Red Soils Harbor Diverse Culturable Actinomycetes That Are Promising Sources of Novel Secondary Metabolites

Guo

Ning

et al. 2015

Appl Environ Microbiol

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Red soils, which are widely distributed in tropical and subtropical regions of southern China, are characterized by low organic carbon, high content of iron oxides, and acidity and, hence, are likely to be ideal habitats for acidophilic actinomycetes. However, the diversity and biosynthetic potential of actinomycetes in such habitats are underexplored. Here, a total of 600 actinomycete strains were isolated from red soils collected in Jiangxi Province in southeast China. 16S rRNA gene sequence analysis revealed a high diversity of the isolates, which were distributed into 26 genera, 10 families, and 7 orders within the class Actinobacteria; these taxa contained at least 49 phylotypes that are likely to represent new species within 15 genera. The isolates showed good physiological potentials for biosynthesis and biocontrol. Chemical screening of 107 semirandomly selected isolates spanning 20 genera revealed the presence of at least 193 secondary metabolites from 52 isolates, of which 125 compounds from 39 isolates of 12 genera were putatively novel. Macrolides, polyethers, diketopiperazines, and siderophores accounted for most of the known compounds. The structures of six novel compounds were elucidated, two of which had a unique skeleton and represented characteristic secondary metabolites of a putative novel Streptomyces phylotype. These results demonstrate that red soils are rich reservoirs for diverse culturable actinomycetes, notably members of the families Streptomycetaceae, Pseudonocardiaceae, and Streptosporangiaceae, with the capacity to synthesize novel bioactive compounds. While high-throughput sequencing methods expand our understanding of species diversity in microbial communities and reveal the presence of many new groups that were previously undetected in cultivation studies, the availability of pure cultures is still essential in order to discover new or useful bioactive compounds from microorganisms. As the most prolific secondary metabolite producers, actinomycetes account for approximately 41% of the bioactive microbial metabolites that have been discovered (1). In recent years, with the emergence of antibiotic-resistant microbial pathogens and rediscovery of known bioactive compounds, special habitats in the ocean (2, 3), sponges (4), plants (5, 6), and insects (7) have been found to be potentially important in the search and discovery of bioactive compound-producing strains. Nevertheless, soil is still the predominant source of secondary metabolite-producing actinomycetes. Unexplored and underexplored soil habitats have been found to be a rich source of actinomycetes that produce novel bioactive metabolites (8-10). Acidic soils in China, mostly red soils, are one such habitat.Red soils are generated by abundant rainfall and high temperature and are widespread in tropical and subtropical regions of southern China, covering about 1.13 million km 2 (11). These soils have been subjected to intensive weathering, and mineral nutrients (including Ca, Mg, P, and K) in the soil are mostly eluted, form...

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confidence: 99%

Red Soils Harbor Diverse Culturable Actinomycetes That Are Promising Sources of Novel Secondary Metabolites

Guo

Ning

et al. 2015

Appl Environ Microbiol

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“…7 A 16S-guided isolation program led to the identification of several new taxa, 7 three of which have been subsequently formally described. [8][9][10] During that program we were also able to isolate hundreds of different strains provisionally designated as 'Alpha' , whose phylogenetic position suggested a new taxon within the Actinobacteria. 7 Independently, phylogenetically related strains have been assigned to the recently described genus Actinoallomurus.…”

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The genus Actinoallomurus and some of its metabolites

Pozzi

Simone²,

Mazzetti³

et al. 2010

J Antibiot

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In the search for novel antibiotics, natural products continue to represent a valid source of bioactive molecules. During a program aimed at identifying previously unreported taxa of actinomycetes as potential source of novel compounds, we isolated hundreds of different representatives of a new group, initially designated as 'Alpha' and independently described as Actinoallomurus. We report on a PCR-specific method for the detection of this taxon, on appropriate growth conditions and on a pilot-screening program on 78 strains. The strains produce antibacterial or antifungal compounds at a relatively high frequency. Four strains were characterized in further detail: one produced the aromatic polyketide benanomicin B and its dexylosyl derivative; a second strain produced N-butylbenzenesulfonamide; a third strain was an efficient converter of soymeal isoflavonoids from soymeal constituents; and a fourth strain produced several coumermycin-related aminocoumarins, with coumermycin A2 as the major peak, and with some new congeners as minor components of the complex. These data suggest that Actinoallomurus strains possess several pathways for secondary metabolism and represent an attractive source in the search for novel antibiotics.

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“…Blastococcus aggregatus Data for reference genera were taken from Li et al (2011), Thawai et al (2006, Couch (1950), Lee & Lee (2011), Lee & Hah (2002), Asano & Kawamoto (1986), Ara et al (2008a, b), Yokota et al (1993), Tamura et al (1994Tamura et al ( , 1997Tamura et al ( , 2001Tamura et al ( , 2006, Thiemann et al (1967), Xie et al (2011), Ara & Kudo, (2007a, Matsumoto et al (2003Matsumoto et al ( , 2014, Ørskov (1923), Inahashi et al (2010), Kane (1966), Wiese et al (2008), Qin et al (2009), Monciardini et al (2009), Maldonado et al (2005, Rheims et al (1998), Jia et al (2013) and Wang et al (2013). +, Present; À, absent.…”

Section: (Ab236957)mentioning

confidence: 99%

Actinorhabdospora filicis gen. nov., sp. nov., a new member of the family Micromonosporaceae

Mingma

Tanaka

Ōmura

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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The actinomycete strains K12-0408 T and K12-0792 were isolated on CM-cellulose agar from rhizosphere soil of a pteridophytic plant collected in Tokyo prefecture, Japan. Their taxonomic positions were determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains K12-0408 T and K12-0792 were positioned within the family Micromonosporaceae. The strains formed extensively branched aerial and substrate mycelia. Long chains of cylindrical spores with smooth surfaces were formed on aerial hyphae. The cell wall contained meso-diaminopimelic acid, and galactose, glucose, mannose and ribose were detected in whole-cell hydrolysates. The predominant menaquinones were MK-10(H 4 ) and MK-10(H 6 ). The major cellular fatty acids were anteiso-C 17 : 0 and anteiso-C 17 : 0 2-OH. The DNA G+C contents of strains K12-0408 T and K12-0792 were 69.6 and 69.7 mol%, respectively. Based on data from the present polyphasic taxonomic study, strains K12-0408 T and K12-0792 represent a novel genus, for which the name Actinorhabdospora gen. nov. is proposed, with strain K12-0408 T (=NBRC 111897 T =TBRC 5327 T ) as the type strain of the type species, Actinorhabdospora filicis sp. nov.The family Micromonosporaceae was first described by Krasil'nikov (1938) and the description was amended by Koch et al. (1996), Stackebrandt et al. (1997) and Zhi et al. (2009) on the basis of chemotaxonomic data and 16S rRNA gene sequence analysis. During the characterization of actinomycetes isolated from rhizospheric soil of fern plants, strains K12-0408 T and K12-0792 were recovered. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences indicated that the two strains were distinct from genera within the family Micromonosporaceae. Herein, we report the results of phenotypic, chemotaxonomic and genomic analyses of strains K12-0408 T and K12-0792, which suggested that the strains represented a novel species of a new genus.Strains K12-0408 T and K12-0792 were isolated from rhizosphere soil of ferns (pteridophyte plants) collected at Fussa and Hamura city, respectively, located in Tokyo prefecture, Japan. The CM-cellulose (CMC) medium used for the isolation procedure contained the following: 1.0 % CM-cellulose sodium salts, 0.1 % K 2 HPO 4 , 0.1 % L-asparagine monohydrate, 0.0001 % FeSO 4 Á7H 2 O, 0.0001 % MnCl 2 Á4H 2 O, 0.0001 % ZnSO 4 Á7H 2 O and 1.5 % agar (adjusted to pH 7.0±0.2 with 1 M NaOH). The medium was supplemented with 0.025 % nalidixic acid and 0.02 % Benlate (Dupont) to minimize and inhibit growth of unwanted bacteria and fungi, respectively. The pure cultures of strains K12-0408 T and K12-0792 were maintained on International Streptomyces Project (ISP) medium 2 (Shirling & Gottlieb, 1966) and in 20 % (v/v) glycerol suspension at À20 C. Biomass for taxonomic characterization was obtained from cultures grown at 27 C for 7 days in yeast extract-dextrose (YD) broth containing 1.0 % yeast extract and 1.0 % glucose.The morphological characteristics of strains K12-0408 T and K12-0792 ...

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Novel members of the family Micromonosporaceae, Rugosimonospora acidiphila gen. nov., sp. nov. and Rugosimonospora africana sp. nov.

Cited by 31 publications

References 30 publications

Red Soils Harbor Diverse Culturable Actinomycetes That Are Promising Sources of Novel Secondary Metabolites

Red Soils Harbor Diverse Culturable Actinomycetes That Are Promising Sources of Novel Secondary Metabolites

The genus Actinoallomurus and some of its metabolites

Actinorhabdospora filicis gen. nov., sp. nov., a new member of the family Micromonosporaceae

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