Novel metabolic pathways of organochlorine pesticides dieldrin and aldrin by the white rot fungi of the genus Phlebia

Xiao, Pengfei; Mori, Toshio; Kamei, Ichiro; Kiyota, Hiromasa; Takagi, Kazuhiro; Kondo, Ryuichiro

doi:10.1016/j.chemosphere.2011.06.028

Cited by 65 publications

(32 citation statements)

References 42 publications

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“…White-rot fungus Phlebia was studied for dieldrin degradation and over 50 % of dieldrin was removed in 42 days which was attributed to hydroxylation reactions in the pathway leading to three hydroxylated metabolite products. These fungi were also found to degrade aldrin (over 90 %) in just 28 days by attacking methylene moiety leading to formation of new metabolites like 9-hydroxyaldrin and two carboxylic acid products [158]. Further, application of proteomics, gene expression studies, and the use of gene-replacement mutants have helped to assign most of the steps in well known but less understood 3-oxaloadipate pathway of aromatic compound degradation to particular genes.…”

Section: Degradation Pathways In Fungimentioning

confidence: 99%

Diverse Metabolic Capacities of Fungi for Bioremediation

2016

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Bioremediation refers to cost-effective and environment-friendly method for converting the toxic, recalcitrant pollutants into environmentally benign products through the action of various biological treatments. Fungi play a major role in bioremediation owing to their robust morphology and diverse metabolic capacity. The review focuses on different fungal groups from a variety of habitats with their role in bioremediation of different toxic and recalcitrant compounds; persistent organic pollutants, textile dyes, effluents from textile, bleached kraft pulp, leather tanning industries, petroleum, polyaromatic hydrocarbons, pharmaceuticals and personal care products, and pesticides. Bioremediation of toxic organics by fungi is the most sustainable and green route for cleanup of contaminated sites and we discuss the multiple modes employed by fungi for detoxification of different toxic and recalcitrant compounds including prominent fungal enzymes viz., catalases, laccases, peroxidases and cyrochrome P450 monooxygeneses. We have also discussed the recent advances in enzyme engineering and genomics and research being carried out to trace the less understood bioremediation pathways.

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Section: Degradation Pathways In Fungimentioning

confidence: 99%

Diverse Metabolic Capacities of Fungi for Bioremediation

2016

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“…Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License new metabolites including 9-hydroxyaldrin and two carboxylic acid products were also detected in the fungal cultures (Xiao et al, 2011).…”

Section: Introductionmentioning

confidence: 77%

“…Thus, aldrin pollution still remains a serious problem in environment and it is required to find some efficient methods for its remediation. Degrading aldrin by microorganisms including bacteria and soil fungi has been reported (Erick et al, 2006;Ferguson et al, 1977;Xiao et al, 2011). The possibility of using Pseudomonas fluorescens cell cultures to biodegrade aldrin in water, obtaining 94.8% degradation of aldrin in 120 h was demonstrated (Erick et al 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Ferguson et al (1977) reported that members of the order Actinomycetales can epoxidize aldrin to exo-dieldrin with their bacterial enzyme system (Ferguson et al 1977). Xiao et al (2011) investigated Phlebia acanthocystis, Phlebia brevispora, and Phlebia aurea to value their degradation capacities on aldrin and the subsequent metabolic products. Over 90% of aldrin was degraded after 28 days and several *Corresponding author.…”

Section: Introductionmentioning

confidence: 99%

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Co-expressing house fly cytochrome P450 6A1 and human cytochrome P450 reductase in Escherichia coli to bioconvert insecticide aldrin

Wu¹,

Li²,

Liang³

et al. 2018

Afr. J. Biotechnol.

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Aldrin used in agricultural as well as industrial or home pest control is known to be a persistent organic pollutant, having chronic adverse effects on wildlife and humans. The degradation of aldrin has drawn a lot of attentions. In this study, house fly cytochrome P450 6A1 (CYP 6A1) or both house fly cytochrome P450 6A1 and human NADPH-P450 reductase (CPR) together was expressed in Escherichia coli cells to study their activity on aldrin conversion. It was found out that the membrane fractions prepared from the recombinant E. coli cells expressing human CPR with CYP 6A1 have catalytic activity on aldrin using enzyme analysis. The catalytic activity of the recombinant E. coli cells was also investigated on aldrin. After adding aldrin to the co-expression of human CPR and CYP 6A1 recombinant E. coli cells culture with 1 µmol L -1 final concentration, 163.36 nmol L -1 of dieldrin was detected after 8 h, the product of aldrin epoxidation. These results indicate that co-expressing house fly CYP 6A1 and human CPR can convert aldrin to dieldrin.

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“…The degradation of diverse pesticides in liquid medium by whole cell ligninolytic fungi or their lignin-modifying enzymes has been reviewed elsewhere (Rodríguez-Rodríguez et al, 2013). In addition, the transformation of organic contaminants by these organisms has been reported in diverse matrices such as sludge, wastewater and soil, not only for pesticides but also in the case of polycyclic aromatic hydrocarbons, industrial dyes and emerging pollutants such as pharmaceuticals and UV filters (D' Annibale et al, 2005;Nilsson et al, 2006;Quintero et al, 2007;Rigas et al, 2007;Xiao et al, 2011).…”

Section: Introductionmentioning

confidence: 97%