Novel metalloprotease–disintegrin, meltrin ϵ (ADAM35), expressed in epithelial tissues during chick embryogenesis

Abstract

Members of the ADAM (a disintegrin and metalloprotease) family are involved in fertilization, morphogenesis, and pathogenesis. Their metalloprotease domains mediate limited proteolysis, including ectodomain shedding of membrane-anchored growth factors and intercellular-signaling proteins, and their disintegrin domains play regulatory roles in cell adhesion and migration. In screening for cDNAs encoding chicken ADAM proteins expressed during muscle development, we identified Meltrin ⑀ as a novel member of this family. To elucidate its functions, we investigated its expression during development by using antibodies raised against its protease domain. In the somites, Meltrin ⑀ protein was specifically expressed in the myotomal cells, which delaminate from the dermomyotome to form epithelial sheets. It was also found in the surface ectoderm, lens placodes, otic vesicles, and the gut epithelia. Basolateral localization of Meltrin ⑀ in these epithelial cells suggests its unique roles in the organization of the epithelial tissues and development of the sensory organs and the gut. Developmental Dynamics 230:557-568, 2004.

Expression patterns of ADAMs in the developing chicken lens

“…In contrast to previous studies (Watabe-Uchida et al 2004;Chen et al 2007), our results show in detail that several ADAMs are expressed throughout the processes of the lens morphogenesis. Each of the ADAMs investigated demonstrates a spatial and temporal expression patterns in the different cell types of the lens with partial overlap between each other.…”

ADAM23 Plays Multiple Roles in Neuronal Differentiation of P19 Embryonal Carcinoma cells

“…There have been 35 members cloned [1,2]. It has been proved that ADAM proteins are involved in many events during development, such as fertilization, myogenesis and neurogenesis [1,[3][4][5].…”

Regionalized expression of ADAM13 during chicken embryonic development

“…These genes are involved in muscle development (Yagami-Hiromasa et al, 1995;Weskamp et al, 1996;Lewis et al, 2004;Watabe-Uchida et al, 2004). The expression of some members of the meltrin subfamily has been described in avian embryos (Lewis et al, 2004;Watabe-Uchida et al, 2004).…”

“…These genes are involved in muscle development (Yagami-Hiromasa et al, 1995;Weskamp et al, 1996;Lewis et al, 2004;Watabe-Uchida et al, 2004). The expression of some members of the meltrin subfamily has been described in avian embryos (Lewis et al, 2004;Watabe-Uchida et al, 2004). In the quail, ADAM12 is expressed in the cranial ganglia, in the mesenchyme of the limb bud, and in the mesenchyme surrounding the dorsal aorta, whereas ADAM19 is expressed in the cranial and dorsal root ganglia and in the allantois (Lewis et al, 2004).…”

“…In the quail, ADAM12 is expressed in the cranial ganglia, in the mesenchyme of the limb bud, and in the mesenchyme surrounding the dorsal aorta, whereas ADAM19 is expressed in the cranial and dorsal root ganglia and in the allantois (Lewis et al, 2004). ADAM35 is expressed in the chicken myotome and several epithelial tissues, such as the otic vesicles and gut epithelia (Watabe-Uchida et al, 2004). The full-length sequence of chicken ADAM9 was reported, but its expression pattern has not been described to date (Caldwell et al, 2005).…”