Novel method of biotyping Haemophilus influenzae that uses API 20e

Abstract

One hundred Haemophilus influenzae isolates from various body sites were biotyped by conventional methods and by the API 20E system (Analytab Products, Plainview, N.Y.). By using a hemin- and a nicotinamide adenine dinucleotide-enriched saline solution as the inoculating fluid for the API 20E, a 100% correlation of results was obtained between the two methods. Ninety percent of the blood and cerebrospinal fluid isolates were biotype I. Biotype II was the predominant biotype encountered overall. No correlation was observed between beta-lactamase production and biotype. The API 20E is a reliable method and should prove useful for routine biotyping of H. influenzae in the clinical laboratory.

Typing of urogenital, maternal, and neonatal isolates of Haemophilus influenzae and Haemophilus parainfluenzae in correlation with clinical source of isolation and evidence for a genital specificity of H. influenzae biotype IV

“…Further identification was carried out by using the API 20E identification system (API Systems, Montalieu Vercieu, France) that includes testing for 11 enzymatic activities and nine carbohydrate fermentations. A full differentiation between H. influenza and H. parainfluenzae can be achieved with API 20E as well as the determination of Kilian's biotypes (12,34,38). For some dubious strains, additional controls of urease, indol, and porphyrin tests were performed.…”

Pneumonia and bacteremia associated with Haemophilus influenzae serotype d

“…The left upper lobe pneumonia promptly responded to ampicillin therapy. This patient was briefly mentioned in a previously published report (9). (ii) Case 2.…”

“…Recent studies emphasize the invasive potential of strains other than serotype b; however, information about these infections is limited (1,5,8,13,22). Except for brief mention (4,7,9,14,21,24), there are few reported case histories of infections due to H. influenzae serotype d (2,18,26).…”