Novel micro-computed tomography contrast agents to visualise the human cardiac conduction system and surrounding structures in hearts from normal, aged, and obese individuals

Aminu, Abimbola J.; Chen, Weixuan; Yin, Zeyuan; Kuniewicz, Marcin; Walocha, Jerzy; Perde, Filip; Molenaar, Peter; Iaizzo, Paul A.; Dobrzynski, Halina; Atkinson, Andrew

doi:10.1016/j.tria.2022.100175

Cited by 6 publications

(7 citation statements)

References 84 publications

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“…Therefore, the segmentation of all recognized blood vessels was highly discontinuous, and small details in branching and smaller vessels could not be visualized due to (i) the limited spatial resolution, (ii) collapse of the blood vessels, or (iii) the lack of blood inside the vessel lumen. X-ray-based visualization of the coronary blood vessel network was demonstrated in previous studies for human hearts using Lugol's iodine and graphene oxide in combination with agarose [47], for rat hearts using phase-contrast imaging [48] and for murine hearts using destructive CESAs [26,27]. The use of Microfil as a casting contrast agent (CCA) was also investigated to visualize the coronary blood vessel network in rats [30] and mice [31,49].…”

Section: Discussionmentioning

confidence: 99%

X-ray-Based 3D Histology of Murine Hearts Using Contrast-Enhanced Microfocus Computed Tomography (CECT) and Cryo-CECT

Pestiaux,

Marino,

Simal

et al. 2023

Hearts

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Cardiovascular diseases are the most common cause of death worldwide, and they still have dramatic consequences on the patients’ lives. Murine models are often used to study the anatomical and microstructural changes caused by the diseases. Contrast-enhanced microfocus computed tomography (CECT) is a new imaging technique for 3D histology of biological tissues. In this study, we confirmed the nondestructiveness of Hf-WD 1:2 POM-based CECT and cryogenic CECT (cryo-CECT) to image the heart in 3D. The influence of the image quality (i.e., acquisition time and spatial resolution) was assessed for the characterization of the heart structural constituents: heart integrity, the coronary blood vessels and the heart valves. Coronary blood vessels were visualized and segmented in murine hearts, allowing us to distinguish veins from arteries and to visualize the 3D spatial distribution of the right coronary artery and the left main coronary artery. Finally, to demonstrate the added value of 3D imaging, the thickness distribution of the two leaflets in the mitral valve and three cusps in the aortic valve was computed in 3D. This study corroborates the added value of CECT and cryo-CECT compared to classical 2D histology to characterize ex vivo the structural properties of murine hearts and paves the way for the detailed 3D (micro)structural analyses of future cardiovascular disease models obtained in mice and rats.

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Section: Discussionmentioning

confidence: 99%

X-ray-Based 3D Histology of Murine Hearts Using Contrast-Enhanced Microfocus Computed Tomography (CECT) and Cryo-CECT

Pestiaux,

Marino,

Simal

et al. 2023

Hearts

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“…4 In comparison to a healthy heart, an aged heart typically has larger chambers, thicker myocardial walls, thicker blood vessels, more extensive nodal regions and connective tissue, increased epicardial fat, and a reduced number of Purkinje fibers. 5 The poem continues:…”

Section: Thinning Of the Skin Thickening Of The Heartmentioning

confidence: 99%

“…Left ventricular hypertrophy is an adaptive response to the increased cardiac afterload caused by age-related arterial dilation and the loss of vascular compliance 4 . In comparison to a healthy heart, an aged heart typically has larger chambers, thicker myocardial walls, thicker blood vessels, more extensive nodal regions and connective tissue, increased epicardial fat, and a reduced number of Purkinje fibers 5 …”

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confidence: 99%

Thinning of the Skin, Thickening of the Heart

Hwang

2024

Journal of Craniofacial Surgery

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“…Unlike in mammalian hearts, there is no identified His-Purkinje network in the amphibian heart. Instead, ventricular trabec- As mentioned above, GO has various applications in different fields, such as a contrast agent in human heart conduction system imaging with micro-computed tomography [77] and in the modeling of prosthetic heart valves [16]. However, it is crucial to note that GO exhibits potential adverse effects on biological systems at different levels.…”

Section: Effects Of Go and Go-peg Without And After Nir-irradiation O...mentioning

confidence: 99%

Comprehensive Assessment of Graphene Oxide Nanoparticles: Effects on Liver Enzymes and Cardiovascular System in Animal Models and Skeletal Muscle Cells

Keremidarska-Markova,

Sazdova,

Ilieva

et al. 2024

Nanomaterials

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The growing interest in graphene oxide (GO) for different biomedical applications requires thoroughly examining its safety. Therefore, there is an urgent need for reliable data on how GO nanoparticles affect healthy cells and organs. In the current work, we adopted a comprehensive approach to assess the influence of GO and its polyethylene glycol-modified form (GO-PEG) under near-infrared (NIR) exposure on several biological aspects. We evaluated the contractility of isolated frog hearts, the activity of two rat liver enzymes–mitochondrial ATPase and diamine oxidase (DAO), and the production of reactive oxygen species (ROS) in C2C12 skeletal muscle cells following direct exposure to GO nanoparticles. The aim was to study the influence of GO nanoparticles at multiple levels—organ; cellular; and subcellular—to provide a broader understanding of their effects. Our data demonstrated that GO and GO-PEG negatively affect heart contractility in frogs, inducing stronger arrhythmic contractions. They increased ROS production in C2C12 myoblasts, whose effects diminished after NIR irradiation. Both nanoparticles in the rat liver significantly stimulated DAO activity, with amplification of this effect after NIR irradiation. GO did not uncouple intact rat liver mitochondria but caused a concentration-dependent decline in ATPase activity in freeze/thaw mitochondria. This multifaceted investigation provides crucial insights into GOs potential for diverse implications in biological systems.

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Novel micro-computed tomography contrast agents to visualise the human cardiac conduction system and surrounding structures in hearts from normal, aged, and obese individuals

Cited by 6 publications

References 84 publications

X-ray-Based 3D Histology of Murine Hearts Using Contrast-Enhanced Microfocus Computed Tomography (CECT) and Cryo-CECT

X-ray-Based 3D Histology of Murine Hearts Using Contrast-Enhanced Microfocus Computed Tomography (CECT) and Cryo-CECT

Thinning of the Skin, Thickening of the Heart

Comprehensive Assessment of Graphene Oxide Nanoparticles: Effects on Liver Enzymes and Cardiovascular System in Animal Models and Skeletal Muscle Cells

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