Novel microcatheter-based intracarotid delivery approach for MCAO/R mice

Cai, Qiang; Chen, Qianxue; Kong, Derek Kai; Wang, Jun; Zhou, Xu; Liu, Baohui; Zhou, Jiangbing

doi:10.1016/j.neulet.2015.04.029

Cited by 10 publications

(12 citation statements)

References 17 publications

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“…The middle cerebral artery occlusion (MCAO) animal model has been widely used to study ischemic brain injury 4 5 6 ; however, most studies indicate that there are no spontaneous post-stroke depressive-like symptoms in the mice 7 8 9 . Current PSD models are largely based on the combination of MCAO and unpredictable chronic mild stress (CMS) 10 11 .…”

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confidence: 99%

Combined use of spatial restraint stress and middle cerebral artery occlusion is a novel model of post-stroke depression in mice

Zhang

Chen

Yang

et al. 2015

Sci Rep

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Post stroke depression (PSD) is one of the most common complications of ischemic stroke. At present, the underlying mechanisms are unclear, largely because there are no reliable, valid and reproducible animal models of PSD. Here we report a novel animal model of PSD that displays consistent and reliable clinical features of hemiplegic stroke. The animal model encompasses a combination of the middle cerebral artery occlusion (MCAO) and spatial restraint stress. We found that a 60-minute MCAO followed by spatial restraint stress for 2 h daily for 2 to 4 weeks from the fourth day after MCAO induced PSD-like depressive phenotypes in mice. Importantly, the mice showed exacerbated deficits of neurological functions and decreased body weights, which were accompanied with reduced levels of brain derived neurotrophic factor and neurotransmitters including serotonin and dopamine. In addition, we identified increased levels of serum cortisol in our PSD mice. Finally, we found that mice with PSD were responsive to the tri-cyclic antidepressant imipramine as evidenced by their attenuated depressive behaviors, increased body weights, recovered brain serotonin levels, and decreased serum cortisol levels. This mouse model replicates multiple features of human post-stroke depression and thus provides a new model for the investigation of PSD.

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confidence: 99%

Combined use of spatial restraint stress and middle cerebral artery occlusion is a novel model of post-stroke depression in mice

Zhang

Chen

Yang

et al. 2015

Sci Rep

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“…However, the neuroprotective effect of DAP on cerebral I/R injury has not been clear and the ability of DAP to antagonize the inflammatory signaling pathway remains to be determined. In order to develop cerebral I/R injury in mice, we established the cerebral I/R mice model induced by MCAO/R [20]. The evaluation of the neurological score and cerebral infarct size indicated that DAP treatment effectively protected against cerebral I/R injury induced by MCAO/R.…”

Section: Discussionmentioning

confidence: 99%

Daphnetin Protects against Cerebral Ischemia/Reperfusion Injury in Mice via Inhibition of TLR4/NF-κB Signaling Pathway

Liu

Chen

Jian

et al. 2016

BioMed Research International

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Growing evidences indicate that immune-mediated mechanisms contribute to the development of cerebral ischemia/reperfusion (I/R) injury. Daphnetin (DAP) is a coumarin derivative extracted from Daphne odora var., which displays anti-inflammatory properties. However, the effect of DAP on cerebral I/R injury is not yet clear. Recent studies have demonstrated that TLR4/NF-κB signaling pathway takes part in the damaging inflammatory process of cerebral I/R injury. The present study aimed to investigate the effect of DAP on cerebral I/R injury in vivo and its possible mechanisms. DAP was administered before middle cerebral artery occlusion and reperfusion in mice. The neurological scores, cerebral infarct sizes, the levels of inflammatory cytokines, apoptotic neural cells, and the levels of TLR4, NF-κB p65, and IκBα were estimated. The results showed that an obvious improvement of neurological scores and infarct sizes was observed in DAP-treated mice after MCAO/R. DAP treatment decreased the overexpression of TNF-α, IL-1β, and IL-6 and attenuated neural cells apoptosis. Moreover, DAP treatment decreased the TLR4 expression, IκB-α degradation, and nuclear translocation of NF-κB. Taken together, our results suggested that DAP exerted neuroprotective and anti-inflammatory effects on cerebral I/R injury. The potential mechanism was involved in the inhibition of TLR4/NF-κB mediated inflammatory signaling pathway.

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“…All animal experiments were approved by the Yale University Institutional Animal Care and Utilization Committee. Stroke was established through MCAO surgery according to our previous reports . TBI was induced through a standard controlled cortical impact (CCI) method.…”

Section: Methodsmentioning

confidence: 99%

Targeted Drug Delivery to Stroke via Chemotactic Recruitment of Nanoparticles Coated with Membrane of Engineered Neural Stem Cells

Zhang

et al. 2019

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Cell membrane coating has recently emerged as a promising biomimetic approach to engineering nanoparticles (NPs) for targeted drug delivery. However, simple cell membrane coating may not meet the need for efficient drug delivery to the brain. Here, a novel molecular engineering strategy to modify the surface of NPs with a cell membrane coating for enhanced brain penetration is reported. By using poly(lactic‐co‐glycolic) acid NPs as a model, it is shown that delivery of NPs to the ischemic brain is enhanced through surface coating with the membrane of neural stem cells (NSCs), and the delivery efficiency can be further increased using membrane isolated from NSCs engineered for overexpression of CXCR4. It is found that this enhancement is mediated by the chemotactic interaction of CXCR4 with SDF‐1, which is enriched in the ischemic microenvironment. It is demonstrated that the resulting CXCR4‐overexpressing membrane‐coated NPs, termed CMNPs, significantly augment the efficacy of glyburide, an anti‐edema agent, for stroke treatment. The study suggests a new approach to improving drug delivery to the ischemic brain and establishes a novel formulation of glyburide that can be potentially translated into clinical applications to improve management of human patients with stroke.

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Novel microcatheter-based intracarotid delivery approach for MCAO/R mice

Cited by 10 publications

References 17 publications

Combined use of spatial restraint stress and middle cerebral artery occlusion is a novel model of post-stroke depression in mice

Combined use of spatial restraint stress and middle cerebral artery occlusion is a novel model of post-stroke depression in mice

Daphnetin Protects against Cerebral Ischemia/Reperfusion Injury in Mice via Inhibition of TLR4/NF-κB Signaling Pathway

Targeted Drug Delivery to Stroke via Chemotactic Recruitment of Nanoparticles Coated with Membrane of Engineered Neural Stem Cells

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