Novel Microfluidic Colon with an Extracellular Matrix Membrane

Wang, Chengyao; Tanataweethum, Nida; Karnik, Sonali; Bhushan, Abhinav

doi:10.1021/acsbiomaterials.7b00883

Cited by 30 publications

(35 citation statements)

References 79 publications

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“…These systems can recapitulate the biochemical, mechanical, structural, and functional features of human organs by mimicking in vivo‐like cellular microenvironment . Researchers have engineered a variety of miniaturized models of human organs, such as liver, lung, intestine, kidney, and heart . These models can potentially bridge the gap between in vitro tests and clinical trials and be utilized for more precise drug prediction in pharmaceutical field in comparison to animal models.…”

Section: Hydrogels In Organs‐on‐a‐chip Engineeringmentioning

confidence: 99%

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

et al. 2019

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The major motivation is to better mimic human physiology and functions at multiscales from the molecular to the cellular, tissue, organ or even whole organism level. Current model systems primarily rely on the monolayer cell cultures and animal models. Simplistic monolayer cultures have their advantages, but they are often significantly different in gene expression, epigenetics, and cell function compared to native 3D tissues. [1,2] Also, they often lack cell-cell and cell-matrix interactions, [2,3] leading to the absence of tissue specific properties. Although animal models are widely used in biomedical research, they fail to faithfully predict human responses in a physiologically relevant manner due to the significant species divergences. [4] The limitations of these systems have provided an impetus for the development of alternative cell-based 3D models in vitro that better resemble the complex functionalities of living organs. Over the last several years, organoids and organ-on-a-chip (OOC), representing the major technological breakthrough, have emerged as two distinct model systems to achieve the same goal of building 3D organotypic models in vitro by bridging the gap between animal models and monolayer cultures. These engineered models are different in terms of cell source, tissue composition, architectural variability, functional features, scales, cellular fidelity, etc. Organoids, primarily evolving from developmental principles, refer to 3D multicellular tissues by self-organization of stem cells or organ-specific progenitors, which can recapitulate the intricate architectures and functionalities of in vivo organs. [5][6][7] Recent breakthroughs in organoid technology have enabled the successful generation of a variety of human organoids, such as the brain, [8,9] intestine, [10,11] liver, [12] kidney, [13,14] lung, [15] etc. These near physiological 3D organoids have garnered momentum for their potential applications in human organ development and disease modeling, drug screening and regenerative medicine. The explosion of organoids research has been catalyzed by the significant progress of stem cell biology and availability of human stem cells. In contrast, the OOC, relying on the bioengineering design principle, is a miniaturized in vitro model that can recreate the functional units of living organs on a microfluidic cell culture device using predifferentiated cells, often cell lines. [1,16] The OOC is primarily evolved from the convergence of tissue engineering and microfabricated technologies, which is characterized by the capability to simulate cellular microenvironment by precise control over fluid flow, mechanical forces and biochemical factors. [4,17] Remarkable progress has been made Significant advances in materials, microscale technology, and stem cell biology have enabled the construction of 3D tissues and organs, which will ultimately lead to more effective diagnostics and therapy. Organoids and organs-on-a-chip (OOC), evolved from developmental biology and bioengineering principles, have e...

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Section: Hydrogels In Organs‐on‐a‐chip Engineeringmentioning

confidence: 99%

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

et al. 2019

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“…The basic device builds upon our previous work in which a microfluidic device with two chambers separated by a porous membrane was developed (Hegde et al, 2014;Wang et al, 2018). Briefly, SYLGARD 184 Silicone Elastomer Kit containing silicone elastomer base and curing agent (Dow Chemical) was used to fabricate the polydimethylsiloxane (PDMS) devices.…”

Section: Methods and Materials: 21 Microfluidic Device Fabricationmentioning

confidence: 99%

A Novel Standalone Microfluidic Device for Local Control of Oxygen Tension for Intestinal-Bacteria Interactions

Wang

Dang

Baste

et al. 2020

Preprint

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The intestinal environment is unique because it supports the intestinal epithelial cells under a normal oxygen environment and the microbiota under an anoxic environment. Due to importance of understanding the interactions between the epithelium and the microbiota, there is a strong need for developing representative and simple experimental models. Current approaches do not capture the dual-oxygen environment, require external anaerobic chambers, or are complex. Another major limitation is that in the solutions that can mimic the dual-oxygen environment, the oxygenation level of the epithelial cells is not known, raising the question whether the cells are hypoxic. We report standalone microfluidic devices that form a dual-oxygen environment without the use of an external anaerobic chamber or oxygen scavengers to coculture intestinal epithelial and bacterial cells. By changing the thickness of the device cover, the oxygen tension in the chamber could be modulated. We verified the oxygen levels using several tests: microscale oxygen sensitive sensors incorporated within the devices, hypoxic immunostaining of Caco-2 cells, and genetically encoded bacteria. Collectively, these methods monitored oxygen concentrations in devices more comprehensively than previous reports and allowed for control of oxygen tension to match the requirements of both intestinal cells and anaerobic bacteria. Our experimental model is supported by the mathematical model that considers diffusion of oxygen into the top chamber and the cellular oxygen consumption rate. This allowed us to experimentally determine the oxygen consumption rate of the epithelial cells more precisely.

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“…graphene and graphene oxide), [15][16][17] polymers (e.g. polypyrrole and polyaniline nanoparticles), [18][19][20][21] inorganic materials (e.g. Se, Sn and Cd with various nanostructures), [22][23][24][25] and their composites.…”

Section: Introductionmentioning

confidence: 99%

Novel approach in synthesizing ternary GO‐Fe₃O₄‐PPy nanocomposites for high Photothermal performance

Getiren

Çıplak

Gökalp

et al. 2019

J of Applied Polymer Sci

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In this study, graphene oxide‐iron oxide‐polypyrrole (GO‐Fe3O4‐PPy) ternary nanocomposites having high photothermal activity and stability are synthesized and analyzed using ultraviolet–visible (UV–Vis) spectroscopy, Fourier transform infrared (FTIR), X‐ray diffraction spectroscopy (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high‐resolution transmission electron microscopy (HRTEM), and magnetic hysteresis measurement (VSM). The effect of power density (1.5–2.5 W cm−2), concentration (0.01–0.2 mg mL−1), and component composition of nanocomposites on the photothermal properties in the near‐infrared (NIR) region (808 nm) is examined. The results show that superparamagnetic GO‐Fe3O4‐PPy ternary nanocomposite exhibits excellent photothermal performance. The temperature reaches to 55.5, 72.3, and 83.1 °C under the irradiation of the 808 nm NIR laser at 1.5, 2.0—, and 2.5 W cm−2 of power density for 10 min at 0.1 mg mL−1 photothermal therapy (PTT) agent, respectively, and moreover it has excellent photothermal stability. In summary, it is concluded that synthesized nanocomposites potentially may be used in simultaneous magnetic field‐guided treatments. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 137, 48837.

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Novel Microfluidic Colon with an Extracellular Matrix Membrane

Cited by 30 publications

References 79 publications

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

A Novel Standalone Microfluidic Device for Local Control of Oxygen Tension for Intestinal-Bacteria Interactions

Novel approach in synthesizing ternary GO‐Fe₃O₄‐PPy nanocomposites for high Photothermal performance

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Novel Microfluidic Colon with an Extracellular Matrix Membrane

Cited by 30 publications

References 79 publications

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

A Novel Standalone Microfluidic Device for Local Control of Oxygen Tension for Intestinal-Bacteria Interactions

Novel approach in synthesizing ternary GO‐Fe3O4‐PPy nanocomposites for high Photothermal performance

Contact Info

Product

Resources

About

Novel approach in synthesizing ternary GO‐Fe₃O₄‐PPy nanocomposites for high Photothermal performance