Novel mucoadhesive polymer prepared by template polymerization of acrylic acid in the presence of silk sericin

Ahn, Jae-Soon; Choi, Hoo‐Kyun; Lee, Ki Hoon; Nahm, Joong‐Hee; Cho, Chong‐Su

doi:10.1002/1097-4628(20010411)80:2<274::aid-app1096>3.0.co;2-g

Cited by 44 publications

(8 citation statements)

References 15 publications

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“…Some of the apparent vibration bands associated with sericin protein amide I arise mainly from primary amides at 1643 to 1644 cm −1 which are ascribed to the C=O stretching vibration [ 23 ]. Amide II bands at 1538 to 1540 cm −1 are ascribed to N–H bending and C–N stretching vibrations, respectively [ 24 ]. All amide III bands found at around 1240 cm −1 are attributed to C-N stretching coupled to N–H bending vibration and usually appear as a weak band [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

Profiling of Silk Sericin from Cocoons of Three Southern African Wild Silk Moths with a Focus on Their Antimicrobial and Antioxidant Properties

et al. 2020

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Silk sericin was extracted from the cocoons of three Southern African wild silk moth species, namely Gonometapostica, G. rufobrunnae (Lepidoptera: Lasiocampidae), and Argema mimosae (Lepidoptera: Saturniidae); these three sericin extracts were analysed to determine the relationship that exists between their chemical structures and their functional properties. The relationship was investigated by utilising several methods that include the determination of the amino acid composition, and characterisation of the secondary structures with Fourier transformation infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The antibacterial properties of these three sericin extracts were evaluated by an agar well diffusion assay with three Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus, and Staphylococcus epidermidis) as test microorganisms; and, lastly, the antioxidant properties of the three sericin extracts were determined using several scavenging methods that include the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS˙+), and the ferric reducing antioxidant power (FRAP) assay. The amino acid composition in the silk sericin extracts from G. postica, G. rufobrunnea, and Argema mimosa in terms of the polar/non-polar ratio (P/NP) was found to be 65:35, 56:44, and 59:41, respectively. The FTIR spectra of these three silk sericin extracts showed distinct major bands such as amide A (3265 cm−1), amide B (3062 cm−1), amide I (1644 cm−1), amide II (1538 cm−1), and amide III (1244 cm−1). The XRD patterns of the silk sericin extracts revealed both amorphous and α-helical structures, with small crystalline regions. All three silk sericin extracts presented potent antibacterial efficacy against the three Gram-positive bacteria and were found to have excellent antioxidant activities against the tested free radicals.

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Section: Resultsmentioning

confidence: 99%

Profiling of Silk Sericin from Cocoons of Three Southern African Wild Silk Moths with a Focus on Their Antimicrobial and Antioxidant Properties

et al. 2020

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“…The chemical composition of sericin powder, chitosan, and sericin-chitosan scaffold were analyzed with Fourier-transform infrared spectroscopy (FTIR) and data collected, as shown in Figure 2. The sericin powder presents peaks at 3280 cm −1 of the -OH group, at 1660 to 1670 cm −1 of amide I, at 1530 to 1540 cm −1 of amide II, and at 1240 cm −1 of amide III [26][27][28]. Sericin powder also presents a peak at 1400 cm −1 which corresponds to hydroxyl groups of serine and tyrosine [28].…”

Section: Characteristic Of Sericin-chitosan Scaffoldmentioning

confidence: 99%

Noncovalent Sericin-Chitosan Scaffold: Physical Properties and Low Cytotoxicity Effect

Chollakup

Uttayarat²,

Chworoś

et al. 2020

IJMS

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This research aims to utilize sericin, which is the waste from boiling silk cocoon, for the supramolecular scaffold preparation with chitosan. A suitable method for the self-assembled scaffold formation of sericin and chitosan at 1:1 stoichiometry is presented and the morphological and physical properties of the scaffold are studied. The effect of an alcohol/NaOH solution on the secondary structure of sericin protein within the sericin-chitosan scaffold, with adjusted pH, was investigated. Additionally, the scaffold was tested in a native phosphate buffer solution (PBS). The results show that sericin increases the porosity of scaffold while chitosan increases the rigidity. The self-assembled sericin and chitosan material is nontoxic to human cells and which can adhere and spread well on such support. For the effect of the molecular weight of chitosan (15,000 and 100,000 g/mol), the scaffold made from lower molecular weight (MW) chitosan provides a somewhat smaller porosity, but a similar swelling ratio and water uptake. On the basis of this research, sericin, which is a silk waste from the textile industry, can be utilized to produce a self-assembled scaffold with chitosan in order to increase the porosity of the scaffold. This type of scaffold is not toxic and can be used for the adhesion of fibroblast cells.

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“…In this regard, the PAA easily leads to an overcharged surface and subsequently stronger electrostatic repulsion between -COOgroups, which cause higher negative zeta-potential [39] . The copolymer has an isoelectric point between pH 4.0 and pH 5.0 due to the pKa of PAA at 4.75 [40] . Therefore, the D-A copolymer displayed a positive zeta potential at pHs below 4.0 due to the protonation of PAA in the acidic media.…”

Section: Resultsmentioning

confidence: 99%

Influence of Ionic Strength on the pH-sensitive in vitro Ibuprofen Release from Dextran-poly(acrylic acid) Copolymer

Zheng¹,

Sun²,

Jin³

et al. 2018

pharmaceutical-sciences

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Zhang, et al.: Influence of Ionic Strength on Ibuprofen ReleaseThe effects of cations and pH on the in vitro ibuprofen release from dextran-poly(acrylic acid) copolymer were investigated at varying pH and Na + or Ca 2+ concentrations. These results revealed that ibuprofen release was strongly depended on the acidic and basic environments, in which, the cumulative amount released in 24 h reached up to 87 % at pH 2.0 but only 32 % at pH 7.4, respectively, demonstrating a good pH-dependency. However, cations in release solution exerted a remarkable influence on ibuprofen release, especially in the acidic media. Meanwhile, ibuprofen release rate decreased with increasing strength of Na + , Ca 2+ and NaCl concentration. The zeta potential profiles and particle distributions at different ionic strengths indicated that cations strongly influence ibuprofen release performance, through surface charge, hydrogen-bonding, and electrostatic interactions in and between the polymer and ibuprofen. These results suggested that Na + and Ca 2+ strength exerted a profound influence on the pH-sensitive in vitro ibuprofen release from the dextranpoly(acrylic acid) copolymer.

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Novel mucoadhesive polymer prepared by template polymerization of acrylic acid in the presence of silk sericin

Cited by 44 publications

References 15 publications

Profiling of Silk Sericin from Cocoons of Three Southern African Wild Silk Moths with a Focus on Their Antimicrobial and Antioxidant Properties

Profiling of Silk Sericin from Cocoons of Three Southern African Wild Silk Moths with a Focus on Their Antimicrobial and Antioxidant Properties

Noncovalent Sericin-Chitosan Scaffold: Physical Properties and Low Cytotoxicity Effect

Influence of Ionic Strength on the pH-sensitive in vitro Ibuprofen Release from Dextran-poly(acrylic acid) Copolymer

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